Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats
Background. Parkinson’s disease (PD) is a common neurological degenerative disease that cannot be completely cured, although drugs can improve or alleviate its symptoms. Optogenetic technology, which stimulates or inhibits neurons with excellent spatial and temporal resolution, provides a new idea a...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2021/9938566 |
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| author | Zongya Zhao Yanxiang Niu Peiqi Chen Yu Zhu Liangliang Shi Xuewei Zhao Chang Wang Yehong Zhang Zhixian Gao Wenshuai Jiang Wu Ren Renjun Gu Yi Yu |
| author_facet | Zongya Zhao Yanxiang Niu Peiqi Chen Yu Zhu Liangliang Shi Xuewei Zhao Chang Wang Yehong Zhang Zhixian Gao Wenshuai Jiang Wu Ren Renjun Gu Yi Yu |
| author_sort | Zongya Zhao |
| collection | DOAJ |
| description | Background. Parkinson’s disease (PD) is a common neurological degenerative disease that cannot be completely cured, although drugs can improve or alleviate its symptoms. Optogenetic technology, which stimulates or inhibits neurons with excellent spatial and temporal resolution, provides a new idea and approach for the precise treatment of Parkinson’s disease. However, the neural mechanism of photogenetic regulation remains unclear. Objective. In this paper, we want to study the nonlinear features of EEG signals in the striatum and globus pallidus through optogenetic stimulation of the substantia nigra compact part. Methods. Rotenone was injected stereotactically into the substantia nigra compact area and ventral tegmental area of SD rats to construct rotenone-treated rats. Then, for the optogenetic manipulation, we injected adeno-associated virus expressing channelrhodopsin to stimulate the globus pallidus and the striatum with a 1 mW blue light and collected LFP signals before, during, and after light stimulation. Finally, the collected LFP signals were analyzed by using nonlinear dynamic algorithms. Results. After observing the behavior and brain morphology, 16 models were finally determined to be successful. LFP results showed that approximate entropy and fractal dimension of rats in the control group were significantly greater than those in the experimental group after light treatment (p<0.05). The LFP nonlinear features in the globus pallidus and striatum of rotenone-treated rats showed significant statistical differences before and after light stimulation (p<0.05). Conclusion. Optogenetic technology can regulate the characteristic value of LFP signals in rotenone-treated rats to a certain extent. Approximate entropy and fractal dimension algorithm can be used as an effective index to study LFP changes in rotenone-treated rats. |
| format | Article |
| id | doaj-art-e9fe60147aa647b9996b2a5bc3215531 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-e9fe60147aa647b9996b2a5bc32155312025-02-03T05:47:08ZengWileyNeural Plasticity2090-59041687-54432021-01-01202110.1155/2021/99385669938566Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated RatsZongya Zhao0Yanxiang Niu1Peiqi Chen2Yu Zhu3Liangliang Shi4Xuewei Zhao5Chang Wang6Yehong Zhang7Zhixian Gao8Wenshuai Jiang9Wu Ren10Renjun Gu11Yi Yu12The Second Affiliated Hospital of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaSchool of Medical Engineering of Xinxiang Medical University, ChinaThe Second Affiliated Hospital of Xinxiang Medical University, ChinaThe Second Affiliated Hospital of Xinxiang Medical University, ChinaBackground. Parkinson’s disease (PD) is a common neurological degenerative disease that cannot be completely cured, although drugs can improve or alleviate its symptoms. Optogenetic technology, which stimulates or inhibits neurons with excellent spatial and temporal resolution, provides a new idea and approach for the precise treatment of Parkinson’s disease. However, the neural mechanism of photogenetic regulation remains unclear. Objective. In this paper, we want to study the nonlinear features of EEG signals in the striatum and globus pallidus through optogenetic stimulation of the substantia nigra compact part. Methods. Rotenone was injected stereotactically into the substantia nigra compact area and ventral tegmental area of SD rats to construct rotenone-treated rats. Then, for the optogenetic manipulation, we injected adeno-associated virus expressing channelrhodopsin to stimulate the globus pallidus and the striatum with a 1 mW blue light and collected LFP signals before, during, and after light stimulation. Finally, the collected LFP signals were analyzed by using nonlinear dynamic algorithms. Results. After observing the behavior and brain morphology, 16 models were finally determined to be successful. LFP results showed that approximate entropy and fractal dimension of rats in the control group were significantly greater than those in the experimental group after light treatment (p<0.05). The LFP nonlinear features in the globus pallidus and striatum of rotenone-treated rats showed significant statistical differences before and after light stimulation (p<0.05). Conclusion. Optogenetic technology can regulate the characteristic value of LFP signals in rotenone-treated rats to a certain extent. Approximate entropy and fractal dimension algorithm can be used as an effective index to study LFP changes in rotenone-treated rats.http://dx.doi.org/10.1155/2021/9938566 |
| spellingShingle | Zongya Zhao Yanxiang Niu Peiqi Chen Yu Zhu Liangliang Shi Xuewei Zhao Chang Wang Yehong Zhang Zhixian Gao Wenshuai Jiang Wu Ren Renjun Gu Yi Yu Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats Neural Plasticity |
| title | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats |
| title_full | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats |
| title_fullStr | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats |
| title_full_unstemmed | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats |
| title_short | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone-Treated Rats |
| title_sort | study on the regulation effect of optogenetic technology on lfp of the basal ganglia nucleus in rotenone treated rats |
| url | http://dx.doi.org/10.1155/2021/9938566 |
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