Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products

Consumer demand for food nutritional content and quality is driving the design of plant-based foods that are enhanced with proteins. In this study, we aimed to reveal the nutrient compositional differences of various states of soy flours. We compared soy protein concentrate (SPC) with full fat (FF),...

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Main Authors: Rahat Bin Robbani, Md. Munnaf Hossen, Kanika Mitra, Md. Zahurul Haque, Md. Abu Zubair, Shumsuzzaman Khan, Md. Nazim Uddin
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2022/9817999
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author Rahat Bin Robbani
Md. Munnaf Hossen
Kanika Mitra
Md. Zahurul Haque
Md. Abu Zubair
Shumsuzzaman Khan
Md. Nazim Uddin
author_facet Rahat Bin Robbani
Md. Munnaf Hossen
Kanika Mitra
Md. Zahurul Haque
Md. Abu Zubair
Shumsuzzaman Khan
Md. Nazim Uddin
author_sort Rahat Bin Robbani
collection DOAJ
description Consumer demand for food nutritional content and quality is driving the design of plant-based foods that are enhanced with proteins. In this study, we aimed to reveal the nutrient compositional differences of various states of soy flours. We compared soy protein concentrate (SPC) with full fat (FF), raw soy flour (RSF), and defatted (DF) soy flour for investigating nutritional content, phytochemicals, and in vitro antioxidant activity. The results showed that the SPC contained significantly (p<0.001) higher protein content (65.14%) and low-fat content (0.54%) than RSF, FF, and DF. Furthermore, the findings revealed that all products contain a significant (ANOVA, p<0.001) amount of essential minerals. The RSF contains significantly higher (p<0.001) potassium (1178.6 mg), calcium (216.77 mg), and magnesium (247 mg) per 100 g than FF, DF, and SPC. SPC contains essential amino acids, but we were unable to detect phenylalanine and tryptophan due to a limitation in the method. Furthermore, using methanolic and aqueous extracts of RSF, FF, DF, and SPC, the flavonoid, phenolics, and antioxidant capacity were also evaluated. According to the findings, soy products in methanolic extract had higher phenolic (about 12-34 mg/g) and flavonoid (about 63-150 mg/g) levels than aqueous extract. Results also demonstrated that FF had higher phenolic content, and SPC had higher flavonoid content than the other products. In vitro models such as phosphomolybdenum blue, FRAP, DPPH, and ABTS assays were used to study the total antioxidant and free radical scavenging potential of soy products, and results found that soy products contained a significant (p<0.001) amount of antioxidant equivalent to gallic acid and vitamin C standard. In the DPPH and ABTS assays, the results also showed that soy products can reduce free radicals in different in vitro models. Altogether, these findings suggest that soy flours, particularly DF and SPC, could be a beneficial food ingredient in the formulation of functional foods.
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spelling doaj-art-e9ebcd49f5cd499bb1bc3a150d38a7ae2025-08-20T02:21:53ZengWileyInternational Journal of Food Science2314-57652022-01-01202210.1155/2022/9817999Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy ProductsRahat Bin Robbani0Md. Munnaf Hossen1Kanika Mitra2Md. Zahurul Haque3Md. Abu Zubair4Shumsuzzaman Khan5Md. Nazim Uddin6Department of Food Technology and Nutritional ScienceInstitute of Food Science and Technology (IFST)Institute of Food Science and Technology (IFST)Institute of Food Science and Technology (IFST)Department of Food Technology and Nutritional ScienceDepartment of Biochemistry and Molecular BiologyInstitute of Food Science and Technology (IFST)Consumer demand for food nutritional content and quality is driving the design of plant-based foods that are enhanced with proteins. In this study, we aimed to reveal the nutrient compositional differences of various states of soy flours. We compared soy protein concentrate (SPC) with full fat (FF), raw soy flour (RSF), and defatted (DF) soy flour for investigating nutritional content, phytochemicals, and in vitro antioxidant activity. The results showed that the SPC contained significantly (p<0.001) higher protein content (65.14%) and low-fat content (0.54%) than RSF, FF, and DF. Furthermore, the findings revealed that all products contain a significant (ANOVA, p<0.001) amount of essential minerals. The RSF contains significantly higher (p<0.001) potassium (1178.6 mg), calcium (216.77 mg), and magnesium (247 mg) per 100 g than FF, DF, and SPC. SPC contains essential amino acids, but we were unable to detect phenylalanine and tryptophan due to a limitation in the method. Furthermore, using methanolic and aqueous extracts of RSF, FF, DF, and SPC, the flavonoid, phenolics, and antioxidant capacity were also evaluated. According to the findings, soy products in methanolic extract had higher phenolic (about 12-34 mg/g) and flavonoid (about 63-150 mg/g) levels than aqueous extract. Results also demonstrated that FF had higher phenolic content, and SPC had higher flavonoid content than the other products. In vitro models such as phosphomolybdenum blue, FRAP, DPPH, and ABTS assays were used to study the total antioxidant and free radical scavenging potential of soy products, and results found that soy products contained a significant (p<0.001) amount of antioxidant equivalent to gallic acid and vitamin C standard. In the DPPH and ABTS assays, the results also showed that soy products can reduce free radicals in different in vitro models. Altogether, these findings suggest that soy flours, particularly DF and SPC, could be a beneficial food ingredient in the formulation of functional foods.http://dx.doi.org/10.1155/2022/9817999
spellingShingle Rahat Bin Robbani
Md. Munnaf Hossen
Kanika Mitra
Md. Zahurul Haque
Md. Abu Zubair
Shumsuzzaman Khan
Md. Nazim Uddin
Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
International Journal of Food Science
title Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
title_full Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
title_fullStr Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
title_full_unstemmed Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
title_short Nutritional, Phytochemical, and In Vitro Antioxidant Activity Analysis of Different States of Soy Products
title_sort nutritional phytochemical and in vitro antioxidant activity analysis of different states of soy products
url http://dx.doi.org/10.1155/2022/9817999
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