IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response
Background. Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response in colon lamina propria and IL-33 induced both regulatory B cells (Bregs) and regulatory T cells (Tregs) in mesenteric...
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| Format: | Article |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/1827901 |
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| author | Jun-feng Zhu Ying Xu Jian Zhao Xue Li Xinrui Meng Tian-qi Wang Ben-yao Zou Peng-yan Zhao Qi Liu Chang-long Lu Fang-liang Zheng Hong-sheng Liu |
| author_facet | Jun-feng Zhu Ying Xu Jian Zhao Xue Li Xinrui Meng Tian-qi Wang Ben-yao Zou Peng-yan Zhao Qi Liu Chang-long Lu Fang-liang Zheng Hong-sheng Liu |
| author_sort | Jun-feng Zhu |
| collection | DOAJ |
| description | Background. Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response in colon lamina propria and IL-33 induced both regulatory B cells (Bregs) and regulatory T cells (Tregs) in mesenteric lymph nodes (MLNs) of mice with DSS-induced acute colitis. Moreover, we speculated that IL-33 would promote the Treg or Breg responses leading to the attenuation of DSS-induced chronic colitis. So, we investigated the role of IL-33 on Bregs and Tregs in the MLN of DSS-induced chronic colitis mice. Methods. IL-33 was administered by intraperitoneal injection to mice with DSS-induced chronic colitis. Clinical symptoms, colon length, and histological changes were determined. The production of cytokines was measured by ELISA. The T and B cell subsets were measured by flow cytometry. The expression of mRNA of transcription factors was measured by quantitative real-time PCR. Results. We show that IL-33 treatment increases both Breg and Treg responses in the MLN of mice with DSS-induced chronic colitis. Moreover, IL-33 treatment also decreases Th17 cell response in the MLN of mice with DSS-induced chronic colitis. Conclusion. Our data provide clear evidence that IL-33 plays a protective role in DSS-induced chronic colitis, which is closely related to increasing Breg and Treg responses in the MLN of mice as well as suppressing Th17 cell responses. |
| format | Article |
| id | doaj-art-e9e4f4888da14962aaa195fc2031c7ef |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e9e4f4888da14962aaa195fc2031c7ef2025-02-03T06:01:04ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/18279011827901IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell ResponseJun-feng Zhu0Ying Xu1Jian Zhao2Xue Li3Xinrui Meng4Tian-qi Wang5Ben-yao Zou6Peng-yan Zhao7Qi Liu8Chang-long Lu9Fang-liang Zheng10Hong-sheng Liu11Life Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaDepartment of Immunology, China Medical University, Shenyang 110013, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaLife Science School, Liaoning University, Shenyang 110036, ChinaBackground. Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response in colon lamina propria and IL-33 induced both regulatory B cells (Bregs) and regulatory T cells (Tregs) in mesenteric lymph nodes (MLNs) of mice with DSS-induced acute colitis. Moreover, we speculated that IL-33 would promote the Treg or Breg responses leading to the attenuation of DSS-induced chronic colitis. So, we investigated the role of IL-33 on Bregs and Tregs in the MLN of DSS-induced chronic colitis mice. Methods. IL-33 was administered by intraperitoneal injection to mice with DSS-induced chronic colitis. Clinical symptoms, colon length, and histological changes were determined. The production of cytokines was measured by ELISA. The T and B cell subsets were measured by flow cytometry. The expression of mRNA of transcription factors was measured by quantitative real-time PCR. Results. We show that IL-33 treatment increases both Breg and Treg responses in the MLN of mice with DSS-induced chronic colitis. Moreover, IL-33 treatment also decreases Th17 cell response in the MLN of mice with DSS-induced chronic colitis. Conclusion. Our data provide clear evidence that IL-33 plays a protective role in DSS-induced chronic colitis, which is closely related to increasing Breg and Treg responses in the MLN of mice as well as suppressing Th17 cell responses.http://dx.doi.org/10.1155/2018/1827901 |
| spellingShingle | Jun-feng Zhu Ying Xu Jian Zhao Xue Li Xinrui Meng Tian-qi Wang Ben-yao Zou Peng-yan Zhao Qi Liu Chang-long Lu Fang-liang Zheng Hong-sheng Liu IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response Journal of Immunology Research |
| title | IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response |
| title_full | IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response |
| title_fullStr | IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response |
| title_full_unstemmed | IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response |
| title_short | IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response |
| title_sort | il 33 protects mice against dss induced chronic colitis by increasing both regulatory b cell and regulatory t cell responses as well as decreasing th17 cell response |
| url | http://dx.doi.org/10.1155/2018/1827901 |
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