Applying reproductive technologies and genome resource banking to laboratory animals
The Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos. Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer. Thirteen mouse and rat strains have been re-derived and 32...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/419 |
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| author | S. Ya. Amstislavsky E. Yu. Brusentsev T. O. Abramova D. S. Ragaeva I. N. Rozhkova T. N. Igonina E. A. Kizilova V. A. Naprimerov N. Yu. Feoktistoiva |
| author_facet | S. Ya. Amstislavsky E. Yu. Brusentsev T. O. Abramova D. S. Ragaeva I. N. Rozhkova T. N. Igonina E. A. Kizilova V. A. Naprimerov N. Yu. Feoktistoiva |
| author_sort | S. Ya. Amstislavsky |
| collection | DOAJ |
| description | The Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos. Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer. Thirteen mouse and rat strains have been re-derived and 32 are kept frozen in the cryostorage at the Institute of Cytology and Genetics, Novosibirsk. Some other laboratory animal species have been cryopreserved as well. Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo (by transfer to a recipient). A positive effect of the granulocyte-macrophage colony-stimulating factor (GM-CSF) was demonstrated with both these Phodopus species. Furthermore, semen of Djungarian (Phodopus sungorus) and Campbell’s (Phodopus campbelli) hamsters, domestic cat (Felis catus), amur cat (Prionailurus bengalensis euptilurus) and bobcat (Lynx rufus) was frozen and cryopreserved. Double staining by SYBR Green/PI and subsequent confocal microscopy demonstrated that more than 40 % of amur cat semen retained viability after cryopreservation. This is the world’s first reported successful freezing of semen of this wild felid (Prionailurus bengalensis euptilurus). This article reviews the results and discusses prospects of using reproductive technologies for conservation of laboratory species. |
| format | Article |
| id | doaj-art-e9d85f0242024726990d1c7995578c8e |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e9d85f0242024726990d1c7995578c8e2025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119436737110.18699/VJ15.045378Applying reproductive technologies and genome resource banking to laboratory animalsS. Ya. Amstislavsky0E. Yu. Brusentsev1T. O. Abramova2D. S. Ragaeva3I. N. Rozhkova4T. N. Igonina5E. A. Kizilova6V. A. Naprimerov7N. Yu. Feoktistoiva8Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaA.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, RussiaThe Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos. Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer. Thirteen mouse and rat strains have been re-derived and 32 are kept frozen in the cryostorage at the Institute of Cytology and Genetics, Novosibirsk. Some other laboratory animal species have been cryopreserved as well. Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo (by transfer to a recipient). A positive effect of the granulocyte-macrophage colony-stimulating factor (GM-CSF) was demonstrated with both these Phodopus species. Furthermore, semen of Djungarian (Phodopus sungorus) and Campbell’s (Phodopus campbelli) hamsters, domestic cat (Felis catus), amur cat (Prionailurus bengalensis euptilurus) and bobcat (Lynx rufus) was frozen and cryopreserved. Double staining by SYBR Green/PI and subsequent confocal microscopy demonstrated that more than 40 % of amur cat semen retained viability after cryopreservation. This is the world’s first reported successful freezing of semen of this wild felid (Prionailurus bengalensis euptilurus). This article reviews the results and discusses prospects of using reproductive technologies for conservation of laboratory species.https://vavilov.elpub.ru/jour/article/view/419cryobanking genetic resourcesembryossemenmiceratshamstersfelids |
| spellingShingle | S. Ya. Amstislavsky E. Yu. Brusentsev T. O. Abramova D. S. Ragaeva I. N. Rozhkova T. N. Igonina E. A. Kizilova V. A. Naprimerov N. Yu. Feoktistoiva Applying reproductive technologies and genome resource banking to laboratory animals Вавиловский журнал генетики и селекции cryobanking genetic resources embryos semen mice rats hamsters felids |
| title | Applying reproductive technologies and genome resource banking to laboratory animals |
| title_full | Applying reproductive technologies and genome resource banking to laboratory animals |
| title_fullStr | Applying reproductive technologies and genome resource banking to laboratory animals |
| title_full_unstemmed | Applying reproductive technologies and genome resource banking to laboratory animals |
| title_short | Applying reproductive technologies and genome resource banking to laboratory animals |
| title_sort | applying reproductive technologies and genome resource banking to laboratory animals |
| topic | cryobanking genetic resources embryos semen mice rats hamsters felids |
| url | https://vavilov.elpub.ru/jour/article/view/419 |
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