The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine
Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitop...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/478052 |
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| author | Yanmin Wan Jing Wang Haizhu Zhou Zhidong Hu Xiaonan Ren Jianqing Xu |
| author_facet | Yanmin Wan Jing Wang Haizhu Zhou Zhidong Hu Xiaonan Ren Jianqing Xu |
| author_sort | Yanmin Wan |
| collection | DOAJ |
| description | Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8+ T cells. We found that frequencies of specific CD8+ T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8+ T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+ T cell receptor. |
| format | Article |
| id | doaj-art-e9c26d8e6e904ce2bdad87329dcb957d |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-e9c26d8e6e904ce2bdad87329dcb957d2025-02-03T06:13:55ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/478052478052The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA VaccineYanmin Wan0Jing Wang1Haizhu Zhou2Zhidong Hu3Xiaonan Ren4Jianqing Xu5Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaSchool of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai 201508, ChinaPrevious studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine. In this study, by constructing epitope-based DNA vaccines encoding a previously identified CD8+ T cell epitope, we investigated the influence of multiplying epitope copies on both the frequency and the MFI of specific IFN-γ secreting CD8+ T cells. We found that frequencies of specific CD8+ T cell could be improved by multiplying epitope copies, while the MFI of IFN-γ secreted by epitope-specific CD8+ T cells decreased synchronously. And further analysis showed that the decrease of MFI was not caused by the functional avidity variation of CD8+ T cell receptor.http://dx.doi.org/10.1155/2012/478052 |
| spellingShingle | Yanmin Wan Jing Wang Haizhu Zhou Zhidong Hu Xiaonan Ren Jianqing Xu The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine Clinical and Developmental Immunology |
| title | The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine |
| title_full | The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine |
| title_fullStr | The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine |
| title_full_unstemmed | The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine |
| title_short | The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine |
| title_sort | average ifn γ secreting capacity of specific cd8 t cells is compromised while increasing copies of a single t cell epitope encoded by dna vaccine |
| url | http://dx.doi.org/10.1155/2012/478052 |
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