Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>)
<i>Aeromonas</i> species are among the main pathogens causing rainbow trout infections. Silver nanoparticles (AgNPs) have a broad spectrum of antimicrobial properties and are usually produced by various green-synthesis methods. However, the application of commercialized AgNPs has not ful...
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2025-01-01
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| author | Yunqiang Guo Chaoli Zheng Yingfei Wang Yongji Dang Ruiyuan Li Ye Tao Yucheng Yang Xiaofeng Sun Zekun Song Pengcheng Sun Qian Zhang Dandan Qian Wenhao Ren Xiyu Cao Bowen Wang Mengxi Xu Bingyang Jiang Yujing Li Qing Sun Jinye Wang Lei Zheng Yanling Sun |
| author_facet | Yunqiang Guo Chaoli Zheng Yingfei Wang Yongji Dang Ruiyuan Li Ye Tao Yucheng Yang Xiaofeng Sun Zekun Song Pengcheng Sun Qian Zhang Dandan Qian Wenhao Ren Xiyu Cao Bowen Wang Mengxi Xu Bingyang Jiang Yujing Li Qing Sun Jinye Wang Lei Zheng Yanling Sun |
| author_sort | Yunqiang Guo |
| collection | DOAJ |
| description | <i>Aeromonas</i> species are among the main pathogens causing rainbow trout infections. Silver nanoparticles (AgNPs) have a broad spectrum of antimicrobial properties and are usually produced by various green-synthesis methods. However, the application of commercialized AgNPs has not fully been clarified. Thus, the objective of this study was to evaluate the antibacterial activities of commercialized AgNPs (range of sizes 10–12 nm) on two contrasting <i>A</i>. <i>salmonicida</i> strains (I-1 and I-4), isolated from rainbow trout; the antibacterial mechanism, histopathological alterations and the expression of immune-related genes were investigated. In vitro, the minimal inhibitory concentration (MIC) was 10 µg/mL for I-1, and lowered to 9.5 µg/mL for I-4, respectively. AgNPs were shown to disrupt both the cell wall and membrane of I-1 and I-4, resulting in cell lysis and degradation. In vivo, rainbow trout challenged by immersed or intraperitoneally injected infection, the 10 µg/mL AgNP-treated groups, both showed delayed deaths and lower mortalities compared to the control groups, without any clinical signs and pathological changes. Especially for the virulent I-4, the enhanced expressions of immune-related genes TNF-α, IL-1β, IL-10 and IL-11 were significantly reduced in the AgNP-treated group, indicating a lesser inflammation due to the application of AgNPs. This study would lay theoretical foundation for the wide application of silver nanoparticles in fish diseases. |
| format | Article |
| id | doaj-art-e9c1dae1b38d4eefb56070358b287d59 |
| institution | Kabale University |
| issn | 2410-3888 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fishes |
| spelling | doaj-art-e9c1dae1b38d4eefb56070358b287d592025-01-24T13:32:30ZengMDPI AGFishes2410-38882025-01-011012910.3390/fishes10010029Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>)Yunqiang Guo0Chaoli Zheng1Yingfei Wang2Yongji Dang3Ruiyuan Li4Ye Tao5Yucheng Yang6Xiaofeng Sun7Zekun Song8Pengcheng Sun9Qian Zhang10Dandan Qian11Wenhao Ren12Xiyu Cao13Bowen Wang14Mengxi Xu15Bingyang Jiang16Yujing Li17Qing Sun18Jinye Wang19Lei Zheng20Yanling Sun21School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Marine Life Sciences, Xiamen Ocean Vocational College, Xiamen 361100, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China<i>Aeromonas</i> species are among the main pathogens causing rainbow trout infections. Silver nanoparticles (AgNPs) have a broad spectrum of antimicrobial properties and are usually produced by various green-synthesis methods. However, the application of commercialized AgNPs has not fully been clarified. Thus, the objective of this study was to evaluate the antibacterial activities of commercialized AgNPs (range of sizes 10–12 nm) on two contrasting <i>A</i>. <i>salmonicida</i> strains (I-1 and I-4), isolated from rainbow trout; the antibacterial mechanism, histopathological alterations and the expression of immune-related genes were investigated. In vitro, the minimal inhibitory concentration (MIC) was 10 µg/mL for I-1, and lowered to 9.5 µg/mL for I-4, respectively. AgNPs were shown to disrupt both the cell wall and membrane of I-1 and I-4, resulting in cell lysis and degradation. In vivo, rainbow trout challenged by immersed or intraperitoneally injected infection, the 10 µg/mL AgNP-treated groups, both showed delayed deaths and lower mortalities compared to the control groups, without any clinical signs and pathological changes. Especially for the virulent I-4, the enhanced expressions of immune-related genes TNF-α, IL-1β, IL-10 and IL-11 were significantly reduced in the AgNP-treated group, indicating a lesser inflammation due to the application of AgNPs. This study would lay theoretical foundation for the wide application of silver nanoparticles in fish diseases.https://www.mdpi.com/2410-3888/10/1/29furunculosisnanoparticlesMICmortalityimmune genes |
| spellingShingle | Yunqiang Guo Chaoli Zheng Yingfei Wang Yongji Dang Ruiyuan Li Ye Tao Yucheng Yang Xiaofeng Sun Zekun Song Pengcheng Sun Qian Zhang Dandan Qian Wenhao Ren Xiyu Cao Bowen Wang Mengxi Xu Bingyang Jiang Yujing Li Qing Sun Jinye Wang Lei Zheng Yanling Sun Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Fishes furunculosis nanoparticles MIC mortality immune genes |
| title | Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full | Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_fullStr | Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full_unstemmed | Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_short | Study on the Role and Pathological and Immune Responses of Silver Nanoparticles Against Two <i>Aeromonas salmonicida</i> subsp. <i>salmonicida</i> Strains at Different Virulence Levels in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_sort | study on the role and pathological and immune responses of silver nanoparticles against two i aeromonas salmonicida i subsp i salmonicida i strains at different virulence levels in rainbow trout i oncorhynchus mykiss i |
| topic | furunculosis nanoparticles MIC mortality immune genes |
| url | https://www.mdpi.com/2410-3888/10/1/29 |
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