Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene
Sustained transgene expression is required for the success of cell transplant-based gene therapy. Most widely used are lentiviral-based vectors which integrate into the host genome and thereby maintain sustained transgene expression. This requires integration into the nuclear genome, and potential r...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/604982 |
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| author | X. Joann You Jing Yang Ping Gu Chee Gee Liew Henry J. Klassen |
| author_facet | X. Joann You Jing Yang Ping Gu Chee Gee Liew Henry J. Klassen |
| author_sort | X. Joann You |
| collection | DOAJ |
| description | Sustained transgene expression is required for the success of cell transplant-based gene therapy. Most widely used are lentiviral-based vectors which integrate into the host genome and thereby maintain sustained transgene expression. This requires integration into the nuclear genome, and potential risks include activation of oncogenes and inactivation of tumor suppressor genes. Plasmids have been used; however lack of sustained expression presents an additional challenge. Here we used the pCAG-PyF101-eGFP plasmid to deliver the human GDNF gene to cat neural progenitor cells (cNPCs). This vector consists of a CAGG composite promoter linked to the polyoma virus mutant enhancer PyF101. Expression of an episomal eGFP reporter and GDNF transgene were stably maintained by the cells, even following induction of differentiation. These genetically modified cells appear suitable for use in allogeneic models of cell-based delivery of GDNF in the cat and may find veterinary applications should such strategies prove clinically beneficial. |
| format | Article |
| id | doaj-art-e9ba45a966f74cdfa5288b99d24e6e20 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e9ba45a966f74cdfa5288b99d24e6e202025-08-20T03:26:34ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/604982604982Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor TransgeneX. Joann You0Jing Yang1Ping Gu2Chee Gee Liew3Henry J. Klassen4The Gavin Herbert Eye Institute, School of Medicine, University of California, Irvine, Hewitt Hall, Rm. 2014, 843 Health Sciences Road, Irvine, CA 92697-1705, USAThe Gavin Herbert Eye Institute, School of Medicine, University of California, Irvine, Hewitt Hall, Rm. 2014, 843 Health Sciences Road, Irvine, CA 92697-1705, USAThe Gavin Herbert Eye Institute, School of Medicine, University of California, Irvine, Hewitt Hall, Rm. 2014, 843 Health Sciences Road, Irvine, CA 92697-1705, USAStem Cell Center, Department of Cell Biology and Neuroscience, University of California Riverside, Riverside, CA 92521, USAThe Gavin Herbert Eye Institute, School of Medicine, University of California, Irvine, Hewitt Hall, Rm. 2014, 843 Health Sciences Road, Irvine, CA 92697-1705, USASustained transgene expression is required for the success of cell transplant-based gene therapy. Most widely used are lentiviral-based vectors which integrate into the host genome and thereby maintain sustained transgene expression. This requires integration into the nuclear genome, and potential risks include activation of oncogenes and inactivation of tumor suppressor genes. Plasmids have been used; however lack of sustained expression presents an additional challenge. Here we used the pCAG-PyF101-eGFP plasmid to deliver the human GDNF gene to cat neural progenitor cells (cNPCs). This vector consists of a CAGG composite promoter linked to the polyoma virus mutant enhancer PyF101. Expression of an episomal eGFP reporter and GDNF transgene were stably maintained by the cells, even following induction of differentiation. These genetically modified cells appear suitable for use in allogeneic models of cell-based delivery of GDNF in the cat and may find veterinary applications should such strategies prove clinically beneficial.http://dx.doi.org/10.1155/2012/604982 |
| spellingShingle | X. Joann You Jing Yang Ping Gu Chee Gee Liew Henry J. Klassen Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene Stem Cells International |
| title | Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene |
| title_full | Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene |
| title_fullStr | Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene |
| title_full_unstemmed | Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene |
| title_short | Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene |
| title_sort | feline neural progenitor cells ii use of novel plasmid vector and hybrid promoter to drive expression of glial cell line derived neurotrophic factor transgene |
| url | http://dx.doi.org/10.1155/2012/604982 |
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