Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper

Abstract Stomatal Closure Point (SCP) has commonly been used to describe drought response strategies in plants, with isohydric species maintaining relatively high, constant SCP compared to anisohydric species that can lower SCP with increasing drought severity. However, there is evidence that, withi...

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Main Authors: Vachel Kraklow, L. Turin Dickman, Max G. Ryan, Emma Lathrop, Jack Heneghan, Dea Musa, Sanna Sevanto
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-00582-6
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author Vachel Kraklow
L. Turin Dickman
Max G. Ryan
Emma Lathrop
Jack Heneghan
Dea Musa
Sanna Sevanto
author_facet Vachel Kraklow
L. Turin Dickman
Max G. Ryan
Emma Lathrop
Jack Heneghan
Dea Musa
Sanna Sevanto
author_sort Vachel Kraklow
collection DOAJ
description Abstract Stomatal Closure Point (SCP) has commonly been used to describe drought response strategies in plants, with isohydric species maintaining relatively high, constant SCP compared to anisohydric species that can lower SCP with increasing drought severity. However, there is evidence that, within these groups, SCP may respond dynamically to environmental conditions. Here, we explored how increasing water availability affects SCP in classically isohydric piñon pine and anisohydric one-seed or Utah juniper at various spatial- (i.e., from branch, to tree, to ecosystem) and temporal- (i.e., hours to decades) scales. Our results show that short-term increases in water availability decreased SCP in isohydric piñon pine, making it more anisohydric, while short-term rehydration had no effect on SCP in anisohydric juniper. Increasing mean annual precipitation, on the other hand, increased SCP in both species. Our findings are consistent with documented differences in the use of ABA to control stomata in iso- and aniso-hydric species on short timescales, and with structural acclimation in both species at long timescales. These results illustrate that the local environment plays a large role in determining SCP.
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spelling doaj-art-e9ae2ccb8a464f98a311167ea4bdb33d2025-08-20T01:51:38ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-00582-6Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniperVachel Kraklow0L. Turin Dickman1Max G. Ryan2Emma Lathrop3Jack Heneghan4Dea Musa5Sanna Sevanto6Earth and Environmental Sciences Division, Los Alamos National LaboratoryEarth and Environmental Sciences Division, Los Alamos National LaboratoryIntegral Ecology GroupEarth and Environmental Sciences Division, Los Alamos National LaboratoryEarth and Environmental Sciences Division, Los Alamos National LaboratoryEarth and Environmental Sciences Division, Los Alamos National LaboratoryEarth and Environmental Sciences Division, Los Alamos National LaboratoryAbstract Stomatal Closure Point (SCP) has commonly been used to describe drought response strategies in plants, with isohydric species maintaining relatively high, constant SCP compared to anisohydric species that can lower SCP with increasing drought severity. However, there is evidence that, within these groups, SCP may respond dynamically to environmental conditions. Here, we explored how increasing water availability affects SCP in classically isohydric piñon pine and anisohydric one-seed or Utah juniper at various spatial- (i.e., from branch, to tree, to ecosystem) and temporal- (i.e., hours to decades) scales. Our results show that short-term increases in water availability decreased SCP in isohydric piñon pine, making it more anisohydric, while short-term rehydration had no effect on SCP in anisohydric juniper. Increasing mean annual precipitation, on the other hand, increased SCP in both species. Our findings are consistent with documented differences in the use of ABA to control stomata in iso- and aniso-hydric species on short timescales, and with structural acclimation in both species at long timescales. These results illustrate that the local environment plays a large role in determining SCP.https://doi.org/10.1038/s41598-025-00582-6Pinus edulisJuniperus monospermaIsohydricAnisohydricDroughtWater availability
spellingShingle Vachel Kraklow
L. Turin Dickman
Max G. Ryan
Emma Lathrop
Jack Heneghan
Dea Musa
Sanna Sevanto
Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
Scientific Reports
Pinus edulis
Juniperus monosperma
Isohydric
Anisohydric
Drought
Water availability
title Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
title_full Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
title_fullStr Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
title_full_unstemmed Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
title_short Increased water availability at various timescales has different effects on stomatal closure point in isohydric piñon pine and anisohydric juniper
title_sort increased water availability at various timescales has different effects on stomatal closure point in isohydric pinon pine and anisohydric juniper
topic Pinus edulis
Juniperus monosperma
Isohydric
Anisohydric
Drought
Water availability
url https://doi.org/10.1038/s41598-025-00582-6
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