Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives
Purpose to study the tumor-forming activity of wild-type MC F-7 cells carrying a full set of porins (VDAC 1, VDAC 2, VDAC 3), as well as their genetically modified cells, from which one of the isoforms was removed (MC F-7 VDAC 1 KO, MC F-7 VDAC 2 KO, MC F -7 VDAC 3 KO).Material and Methods. The stud...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2022-03-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/2029 |
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| author | E. Yu. Mndlyan S. G. Semushina D. I. Rzhevsky N. I. Novikova E. A. Kalabina D. S. Komkov A. Yu. Maslennikova A. N. Murashev E. L. Holmuhamedov |
| author_facet | E. Yu. Mndlyan S. G. Semushina D. I. Rzhevsky N. I. Novikova E. A. Kalabina D. S. Komkov A. Yu. Maslennikova A. N. Murashev E. L. Holmuhamedov |
| author_sort | E. Yu. Mndlyan |
| collection | DOAJ |
| description | Purpose to study the tumor-forming activity of wild-type MC F-7 cells carrying a full set of porins (VDAC 1, VDAC 2, VDAC 3), as well as their genetically modified cells, from which one of the isoforms was removed (MC F-7 VDAC 1 KO, MC F-7 VDAC 2 KO, MC F -7 VDAC 3 KO).Material and Methods. The study was aimed at establishing of an animal model of orthotopic tumors in the mammary gland of immunodeficient BAL B/c nude mice by implanting a suspension of human breast cancer cells (MC F-7) and derivatives of these cells generated by targeted knockout of one of the selected mitochondrial porin isoforms (VDAC 1, VDAC 2 or VDAC 3). Suspensions of either wild-type MC F-7 cell lines containing all three porin isoforms (VDAC 1, VDAC 2 and VDAC 3) or their VDAC -deficient derivatives (MC F-7 VDAC 1 KO, MC F-7 VDAC 2 KO and MC F-7 VDAC 3 KO) were injected into mammary fat pads of BAL B/c nude mice at a dose of 4x106 cells per injection. A pathomorphological analysis of the place of implantation of tumor cells, the tumor itself, as well as the organs of the abdominal and thoracic cavity was carried out.Results. The study shows the feasibility of successful creation of orthotopic tumors in the adipose tissue of immunodeficient BAL B/c nude mice with MC F-7 human breast cancer epithelial cells containing a complete set of mitochondrial porin isoforms and their VDAC -deficient derivatives. The tumor-forming activity of the implanted cells was shown to correlate with their cytotoxic effect on the internal organs of animals. Pathological analysis showed that all implanted cell cultures, such as MC F-7 WT, MC F-7 VDAC 2 KO and MC F-7 VDAC 3 KO, except for MC F-7 VDAC 1 KO cells, which did not form tumors, caused pathological changes in the lungs, liver and spleen, as well as the presence of other tumor-like lesions.Conclusion. The data obtained will be used to optimize the injection volume and cell number, as well as to refine the dynamics of tumor growth, suitable for studying the effect of anticancer drugs on tumors formed by human breast cancer cells (MC F-7) and its genetically modified VDAC -deficient derivatives. |
| format | Article |
| id | doaj-art-e99ce09344314ef0981a3ece7fe98dff |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2022-03-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-e99ce09344314ef0981a3ece7fe98dff2025-08-20T03:43:40ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682022-03-01211728410.21294/1814-4861-2022-21-1-72-84962Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivativesE. Yu. Mndlyan0S. G. Semushina1D. I. Rzhevsky2N. I. Novikova3E. A. Kalabina4D. S. Komkov5A. Yu. Maslennikova6A. N. Murashev7E. L. Holmuhamedov8FGBUN Institute of Theoretical and Experimental Biophysics, RASBranch of the FGBUN Institute of Bioorganic Chemistry named after academicians M.M. Shemyakin and Yu.A. Ovchinnikov RASBranch of the FGBUN Institute of Bioorganic Chemistry named after academicians M.M. Shemyakin and Yu.A. Ovchinnikov RASBranch of the FGBUN Institute of Bioorganic Chemistry named after academicians M.M. Shemyakin and Yu.A. Ovchinnikov RASBranch of the FGBUN Institute of Bioorganic Chemistry named after academicians M.M. Shemyakin and Yu.A. Ovchinnikov RASFGBUN Institute of Gene Biology, RASFGBUN Institute of Gene Biology, RASBranch of the FGBUN Institute of Bioorganic Chemistry named after academicians M.M. Shemyakin and Yu.A. Ovchinnikov RASFGBUN Institute of Theoretical and Experimental Biophysics, RASPurpose to study the tumor-forming activity of wild-type MC F-7 cells carrying a full set of porins (VDAC 1, VDAC 2, VDAC 3), as well as their genetically modified cells, from which one of the isoforms was removed (MC F-7 VDAC 1 KO, MC F-7 VDAC 2 KO, MC F -7 VDAC 3 KO).Material and Methods. The study was aimed at establishing of an animal model of orthotopic tumors in the mammary gland of immunodeficient BAL B/c nude mice by implanting a suspension of human breast cancer cells (MC F-7) and derivatives of these cells generated by targeted knockout of one of the selected mitochondrial porin isoforms (VDAC 1, VDAC 2 or VDAC 3). Suspensions of either wild-type MC F-7 cell lines containing all three porin isoforms (VDAC 1, VDAC 2 and VDAC 3) or their VDAC -deficient derivatives (MC F-7 VDAC 1 KO, MC F-7 VDAC 2 KO and MC F-7 VDAC 3 KO) were injected into mammary fat pads of BAL B/c nude mice at a dose of 4x106 cells per injection. A pathomorphological analysis of the place of implantation of tumor cells, the tumor itself, as well as the organs of the abdominal and thoracic cavity was carried out.Results. The study shows the feasibility of successful creation of orthotopic tumors in the adipose tissue of immunodeficient BAL B/c nude mice with MC F-7 human breast cancer epithelial cells containing a complete set of mitochondrial porin isoforms and their VDAC -deficient derivatives. The tumor-forming activity of the implanted cells was shown to correlate with their cytotoxic effect on the internal organs of animals. Pathological analysis showed that all implanted cell cultures, such as MC F-7 WT, MC F-7 VDAC 2 KO and MC F-7 VDAC 3 KO, except for MC F-7 VDAC 1 KO cells, which did not form tumors, caused pathological changes in the lungs, liver and spleen, as well as the presence of other tumor-like lesions.Conclusion. The data obtained will be used to optimize the injection volume and cell number, as well as to refine the dynamics of tumor growth, suitable for studying the effect of anticancer drugs on tumors formed by human breast cancer cells (MC F-7) and its genetically modified VDAC -deficient derivatives.https://www.siboncoj.ru/jour/article/view/2029cancer cells mcf-7mitochondrial porins (vdac1vdac2vdac3)immunodeficient balb / c nude miceorthotopic tumor |
| spellingShingle | E. Yu. Mndlyan S. G. Semushina D. I. Rzhevsky N. I. Novikova E. A. Kalabina D. S. Komkov A. Yu. Maslennikova A. N. Murashev E. L. Holmuhamedov Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives Сибирский онкологический журнал cancer cells mcf-7 mitochondrial porins (vdac1 vdac2 vdac3) immunodeficient balb / c nude mice orthotopic tumor |
| title | Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives |
| title_full | Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives |
| title_fullStr | Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives |
| title_full_unstemmed | Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives |
| title_short | Establishment of an orthotopic tumor model in the mammary gland of BALB/C nude mice using human breast cancer MCF-7 cells and their VDAC-deficient derivatives |
| title_sort | establishment of an orthotopic tumor model in the mammary gland of balb c nude mice using human breast cancer mcf 7 cells and their vdac deficient derivatives |
| topic | cancer cells mcf-7 mitochondrial porins (vdac1 vdac2 vdac3) immunodeficient balb / c nude mice orthotopic tumor |
| url | https://www.siboncoj.ru/jour/article/view/2029 |
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