Interpretable AI-driven multidimensional chemical fingerprints for geographical authentication of Euryales Semen

Abstract Euryales Semen (ES, Euryale ferox Salisb.) is a valuable aquatic food in Asia. Its quality and price depend on its geographical origins. To ensure the authenticity of ES, a tracing strategy using stable isotopes, elements, and starch composition with interpretable algorithms was successfull...

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Bibliographic Details
Main Authors: Daixin Yu, Cheng Qu, Jing Nie, Pei Wen, Yuyang Zhao, Caiyan Dai, Hui Yan, Yuwei Yuan, Qinan Wu
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:npj Science of Food
Online Access:https://doi.org/10.1038/s41538-025-00510-y
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Summary:Abstract Euryales Semen (ES, Euryale ferox Salisb.) is a valuable aquatic food in Asia. Its quality and price depend on its geographical origins. To ensure the authenticity of ES, a tracing strategy using stable isotopes, elements, and starch composition with interpretable algorithms was successfully developed. Results indicated that ES from different regions exhibited different chemical fingerprinting profiles. Tree-based intelligent algorithms were introduced for classification, and light gradient boosting machine (LightGBM) achieved the highest accuracy of 97.67%. The SHapley Additive exPlanation (SHAP) interpreted the LightGBM output for feature impact. Notably, the top 10 significant variables, encompassing Na, V, Ba, Sb, Cu, Ti, Mn, %N, amylose, and ratio of amylose to amylopectin (SHAP value >1.0), were selected as the key factors. Moreover, environmental factors were found to be significantly related to these key variables (p < 0.05). Overall, this study offers an effective strategy for the geographical origin traceability of ES or other aquatic crops.
ISSN:2396-8370