Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus
Biofloc technology (BFT) has recently attracted great attention due to minimal water exchange and reduced feed intake. This study aimed to recognize daily changes in the digestive physiology of Oreochromis niloticus between a traditional system and BFT. The enzyme activity of trypsin (try), chymotry...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/anu/6617425 |
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| author | María del Carmen Monroy-Dosta Daniel Becerril-Cortés Juan Pablo Lazo Arturo Mena-López Pilar Negrete-Redondo Eliasid Nogueda-Torres Carmen Navarro-Guillén José Antonio Mata-Sotres |
| author_facet | María del Carmen Monroy-Dosta Daniel Becerril-Cortés Juan Pablo Lazo Arturo Mena-López Pilar Negrete-Redondo Eliasid Nogueda-Torres Carmen Navarro-Guillén José Antonio Mata-Sotres |
| author_sort | María del Carmen Monroy-Dosta |
| collection | DOAJ |
| description | Biofloc technology (BFT) has recently attracted great attention due to minimal water exchange and reduced feed intake. This study aimed to recognize daily changes in the digestive physiology of Oreochromis niloticus between a traditional system and BFT. The enzyme activity of trypsin (try), chymotrypsin (chy), leucine aminopeptidase (lap), alkaline proteases (alk), lipase (lip), and amylase (amy), along with the gene expression of trypsin (try), chymotrypsin (chy), pepsin (pep), amylase (amy), and phospholipase (pla) were measured throughout a daily cycle. Samples were taken every 4 h in a 24 h cycle under a 12:12 L:D photoperiod. During 60 days, fish were feed three times a day (zeitgeber time, ZT: 0, 4, and 8) with a fishmeal-based diet containing 32% of crude protein and 5% of lipid, where molasses was added as a carbon source in BFT. No significant differences were found in fish performance among treatments at the end of the experiment. The activity of all tested enzymes significantly (p<0.05) increases during the dark period in both treatments, where the same activity pattern was found in try and lip. The maximum expression levels of digestive gene enzymes between treatments show a marked effect dependent on the presence of light and dark phases. The cosinor analysis showed an activity in try, lap, and lip with a significant rhythmicity (p<0.05). Our results demonstrate that some processes related to the digestive physiology of tilapia that respond directly to daily rhythmicity are modified under the constant presence of feed in BFT. These findings should be considered when establishing new optimized culture protocols. |
| format | Article |
| id | doaj-art-e9822f7a9e5e477f9ba3af27af007e95 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-e9822f7a9e5e477f9ba3af27af007e952025-01-31T00:00:04ZengWileyAquaculture Nutrition1365-20952025-01-01202510.1155/anu/6617425Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticusMaría del Carmen Monroy-Dosta0Daniel Becerril-Cortés1Juan Pablo Lazo2Arturo Mena-López3Pilar Negrete-Redondo4Eliasid Nogueda-Torres5Carmen Navarro-Guillén6José Antonio Mata-Sotres7Departamento El Hombre y su AmbienteDepartamento El Hombre y su AmbienteCentro de Investigación Científica y de Educación Superior de Ensenada (CICESE)Departamento El Hombre y su AmbienteDepartamento El Hombre y su AmbienteCentro de Investigación Científica y de Educación Superior de Ensenada (CICESE)Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC)Departamento El Hombre y su AmbienteBiofloc technology (BFT) has recently attracted great attention due to minimal water exchange and reduced feed intake. This study aimed to recognize daily changes in the digestive physiology of Oreochromis niloticus between a traditional system and BFT. The enzyme activity of trypsin (try), chymotrypsin (chy), leucine aminopeptidase (lap), alkaline proteases (alk), lipase (lip), and amylase (amy), along with the gene expression of trypsin (try), chymotrypsin (chy), pepsin (pep), amylase (amy), and phospholipase (pla) were measured throughout a daily cycle. Samples were taken every 4 h in a 24 h cycle under a 12:12 L:D photoperiod. During 60 days, fish were feed three times a day (zeitgeber time, ZT: 0, 4, and 8) with a fishmeal-based diet containing 32% of crude protein and 5% of lipid, where molasses was added as a carbon source in BFT. No significant differences were found in fish performance among treatments at the end of the experiment. The activity of all tested enzymes significantly (p<0.05) increases during the dark period in both treatments, where the same activity pattern was found in try and lip. The maximum expression levels of digestive gene enzymes between treatments show a marked effect dependent on the presence of light and dark phases. The cosinor analysis showed an activity in try, lap, and lip with a significant rhythmicity (p<0.05). Our results demonstrate that some processes related to the digestive physiology of tilapia that respond directly to daily rhythmicity are modified under the constant presence of feed in BFT. These findings should be considered when establishing new optimized culture protocols.http://dx.doi.org/10.1155/anu/6617425 |
| spellingShingle | María del Carmen Monroy-Dosta Daniel Becerril-Cortés Juan Pablo Lazo Arturo Mena-López Pilar Negrete-Redondo Eliasid Nogueda-Torres Carmen Navarro-Guillén José Antonio Mata-Sotres Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus Aquaculture Nutrition |
| title | Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus |
| title_full | Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus |
| title_fullStr | Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus |
| title_full_unstemmed | Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus |
| title_short | Effect of Biofloc Culture on the Daily Rhythmicity of the Activity and Expression of Digestive Enzymes in Tilapia, Oreochromis niloticus |
| title_sort | effect of biofloc culture on the daily rhythmicity of the activity and expression of digestive enzymes in tilapia oreochromis niloticus |
| url | http://dx.doi.org/10.1155/anu/6617425 |
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