Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells
Summary: Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated,...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007573 |
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| author | Sherry Li Zheng Jonas L. Fowler Julie Y. Chen Christopher Li Elaine Lin Alana T. Nguyen Angela Chen George Q. Daley Lay Teng Ang Kyle M. Loh |
| author_facet | Sherry Li Zheng Jonas L. Fowler Julie Y. Chen Christopher Li Elaine Lin Alana T. Nguyen Angela Chen George Q. Daley Lay Teng Ang Kyle M. Loh |
| author_sort | Sherry Li Zheng |
| collection | DOAJ |
| description | Summary: Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated, seeded, and then sequentially differentiated into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and hematopoietic progenitors through the sequential addition of defined, serum-free media. This 10-day protocol enables the manufacturing of blood and immune cells in monolayer cultures.For complete details on the use and execution of this protocol, please refer to Fowler and Zheng et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-e972cd753a304fbea002e964ad12d71c |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-e972cd753a304fbea002e964ad12d71c2025-01-26T05:04:56ZengElsevierSTAR Protocols2666-16672025-03-0161103592Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cellsSherry Li Zheng0Jonas L. Fowler1Julie Y. Chen2Christopher Li3Elaine Lin4Alana T. Nguyen5Angela Chen6George Q. Daley7Lay Teng Ang8Kyle M. Loh9Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA; Corresponding authorInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USAStem Cell Program, Boston Children’s Hospital, Boston, MA 02115, USA; Division of Hematology/Oncology, Boston Children’s Hospital and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Belfer Center for Science and International Affairs, Harvard Kennedy School, Cambridge, MA 02138, USAStem Cell Program, Boston Children’s Hospital, Boston, MA 02115, USA; Division of Hematology/Oncology, Boston Children’s Hospital and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USAStem Cell Program, Boston Children’s Hospital, Boston, MA 02115, USA; Division of Hematology/Oncology, Boston Children’s Hospital and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Urology, Stanford University, Stanford, CA 94305, USAInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA; Corresponding authorSummary: Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated, seeded, and then sequentially differentiated into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and hematopoietic progenitors through the sequential addition of defined, serum-free media. This 10-day protocol enables the manufacturing of blood and immune cells in monolayer cultures.For complete details on the use and execution of this protocol, please refer to Fowler and Zheng et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007573cell differentiationdevelopmental biologystem cells |
| spellingShingle | Sherry Li Zheng Jonas L. Fowler Julie Y. Chen Christopher Li Elaine Lin Alana T. Nguyen Angela Chen George Q. Daley Lay Teng Ang Kyle M. Loh Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells STAR Protocols cell differentiation developmental biology stem cells |
| title | Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells |
| title_full | Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells |
| title_fullStr | Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells |
| title_full_unstemmed | Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells |
| title_short | Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells |
| title_sort | protocol for the generation of hlf hoxa human hematopoietic progenitor cells from pluripotent stem cells |
| topic | cell differentiation developmental biology stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007573 |
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