Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids
Oxysterols and bile acids are interconnected bioactive lipids playing pivotal roles in diverse physiological and pathological processes. For this reason, they are increasingly studied together for their implications in various diseases. However, due to analytical challenges inherent to the nature of...
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Elsevier
2025-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227524002025 |
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| author | Martin Roumain Giulio G. Muccioli |
| author_facet | Martin Roumain Giulio G. Muccioli |
| author_sort | Martin Roumain |
| collection | DOAJ |
| description | Oxysterols and bile acids are interconnected bioactive lipids playing pivotal roles in diverse physiological and pathological processes. For this reason, they are increasingly studied together for their implications in various diseases. However, due to analytical challenges inherent to the nature of these analytes, very few methods have been developed for the simultaneous analysis of these lipids. We here report the development of a sensitive LC-MS/MS method for the combined quantification of 18 oxysterols, 11 unconjugated, 15 conjugated bile acids, and 1 bile acid precursor, using 8 isotope-labeled internal standards, addressing the need for a more comprehensive analysis of these interesting lipid families. During the method development, we investigated different extraction protocols, set up a purification step, and achieved chromatographic separation for these lipids, overcoming challenges such as the large number of analytes, isomers, and wide range of polarity across the analytes. Finally, the method was successfully applied to the analysis of preclinical and clinical samples, quantifying 12 oxysterols and 14 bile acids in human plasma, 10 oxysterols and 18 bile acids in mouse plasma from the vena cava, and 10 oxysterols and 20 bile acids in mouse plasma from the portal vein within a single chromatographic run. |
| format | Article |
| id | doaj-art-e939c9bf39a04689a72a72ffc8c82c97 |
| institution | Kabale University |
| issn | 0022-2275 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj-art-e939c9bf39a04689a72a72ffc8c82c972025-01-30T05:12:35ZengElsevierJournal of Lipid Research0022-22752025-01-01661100697Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acidsMartin Roumain0Giulio G. Muccioli1Bioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, BelgiumFor correspondence: Giulio G. Muccioli; Bioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, BelgiumOxysterols and bile acids are interconnected bioactive lipids playing pivotal roles in diverse physiological and pathological processes. For this reason, they are increasingly studied together for their implications in various diseases. However, due to analytical challenges inherent to the nature of these analytes, very few methods have been developed for the simultaneous analysis of these lipids. We here report the development of a sensitive LC-MS/MS method for the combined quantification of 18 oxysterols, 11 unconjugated, 15 conjugated bile acids, and 1 bile acid precursor, using 8 isotope-labeled internal standards, addressing the need for a more comprehensive analysis of these interesting lipid families. During the method development, we investigated different extraction protocols, set up a purification step, and achieved chromatographic separation for these lipids, overcoming challenges such as the large number of analytes, isomers, and wide range of polarity across the analytes. Finally, the method was successfully applied to the analysis of preclinical and clinical samples, quantifying 12 oxysterols and 14 bile acids in human plasma, 10 oxysterols and 18 bile acids in mouse plasma from the vena cava, and 10 oxysterols and 20 bile acids in mouse plasma from the portal vein within a single chromatographic run.http://www.sciencedirect.com/science/article/pii/S0022227524002025lipidomicslipidssterolscholesterol metabolismcytochrome P450liquid chromatography mass spectrometry |
| spellingShingle | Martin Roumain Giulio G. Muccioli Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids Journal of Lipid Research lipidomics lipids sterols cholesterol metabolism cytochrome P450 liquid chromatography mass spectrometry |
| title | Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids |
| title_full | Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids |
| title_fullStr | Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids |
| title_full_unstemmed | Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids |
| title_short | Development and application of an LC-MS/MS method for the combined quantification of oxysterols and bile acids |
| title_sort | development and application of an lc ms ms method for the combined quantification of oxysterols and bile acids |
| topic | lipidomics lipids sterols cholesterol metabolism cytochrome P450 liquid chromatography mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S0022227524002025 |
| work_keys_str_mv | AT martinroumain developmentandapplicationofanlcmsmsmethodforthecombinedquantificationofoxysterolsandbileacids AT giuliogmuccioli developmentandapplicationofanlcmsmsmethodforthecombinedquantificationofoxysterolsandbileacids |