Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface
Abstract Regenerating natural gradients of the tendon‒bone interface (TBI) is a major challenge in the reconstruction of rotator cuff tear (RCT). In this study, magnetic Janus hydrogel microrobots to match the TBI orientation during RCT reconstruction surgery are developed via a biofriendly gas-shea...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57499-x |
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| author | Zichuan Ding Yongrui Cai Haocheng Sun Xiao Rong Sipei Ye Jiaxuan Fan Yahao Lai Zhimin Liang Chao Huang Peilin Li Xiaoxue Fu Liu Wang Guosheng Tang Zongke Zhou Zeyu Luo |
| author_facet | Zichuan Ding Yongrui Cai Haocheng Sun Xiao Rong Sipei Ye Jiaxuan Fan Yahao Lai Zhimin Liang Chao Huang Peilin Li Xiaoxue Fu Liu Wang Guosheng Tang Zongke Zhou Zeyu Luo |
| author_sort | Zichuan Ding |
| collection | DOAJ |
| description | Abstract Regenerating natural gradients of the tendon‒bone interface (TBI) is a major challenge in the reconstruction of rotator cuff tear (RCT). In this study, magnetic Janus hydrogel microrobots to match the TBI orientation during RCT reconstruction surgery are developed via a biofriendly gas-shearing microfluidic platform. Through separate loading of Mg2+ and Zn2+, the microrobots facilitate the immediate restoration and long-term maintenance of the natural mineral gradient in the TBI after implantation and alignment through magnetic manipulation. In vitro studies confirm the spatiotemporal cell phenotype modulation effects of the microrobots. In a rat RCT model, microrobots synchronously promote the bone and tendon regeneration, and the restoration of gradient tendon‒bone transition structures in the TBI. Overall, by rebuilding the Mg2+/Zn2+ mineral gradient, cell phenotype gradient and structural gradient of the TBI, magnetic Janus microrobots loaded with dual bioactive ions represent a promising strategy for promoting TBI healing in RCT reconstruction surgery. |
| format | Article |
| id | doaj-art-e91f95e0e90a4e65be087add773d84e5 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e91f95e0e90a4e65be087add773d84e52025-08-20T03:05:55ZengNature PortfolioNature Communications2041-17232025-03-0116111910.1038/s41467-025-57499-xJanus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interfaceZichuan Ding0Yongrui Cai1Haocheng Sun2Xiao Rong3Sipei Ye4Jiaxuan Fan5Yahao Lai6Zhimin Liang7Chao Huang8Peilin Li9Xiaoxue Fu10Liu Wang11Guosheng Tang12Zongke Zhou13Zeyu Luo14Orthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityDepartment of Medical Ultrasound, West China Hospital, Sichuan UniversityCAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of ChinaOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityWest China School of Nursing, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityState Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityCAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of ChinaGuangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, the Fifth Affiliated Hospital and School of Pharmaceutical Sciences, Guangzhou Medical UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityOrthopaedic Research Institute, Department of Orthopaedics, West China Hospital, Sichuan UniversityAbstract Regenerating natural gradients of the tendon‒bone interface (TBI) is a major challenge in the reconstruction of rotator cuff tear (RCT). In this study, magnetic Janus hydrogel microrobots to match the TBI orientation during RCT reconstruction surgery are developed via a biofriendly gas-shearing microfluidic platform. Through separate loading of Mg2+ and Zn2+, the microrobots facilitate the immediate restoration and long-term maintenance of the natural mineral gradient in the TBI after implantation and alignment through magnetic manipulation. In vitro studies confirm the spatiotemporal cell phenotype modulation effects of the microrobots. In a rat RCT model, microrobots synchronously promote the bone and tendon regeneration, and the restoration of gradient tendon‒bone transition structures in the TBI. Overall, by rebuilding the Mg2+/Zn2+ mineral gradient, cell phenotype gradient and structural gradient of the TBI, magnetic Janus microrobots loaded with dual bioactive ions represent a promising strategy for promoting TBI healing in RCT reconstruction surgery.https://doi.org/10.1038/s41467-025-57499-x |
| spellingShingle | Zichuan Ding Yongrui Cai Haocheng Sun Xiao Rong Sipei Ye Jiaxuan Fan Yahao Lai Zhimin Liang Chao Huang Peilin Li Xiaoxue Fu Liu Wang Guosheng Tang Zongke Zhou Zeyu Luo Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface Nature Communications |
| title | Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface |
| title_full | Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface |
| title_fullStr | Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface |
| title_full_unstemmed | Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface |
| title_short | Janus hydrogel microrobots with bioactive ions for the regeneration of tendon-bone interface |
| title_sort | janus hydrogel microrobots with bioactive ions for the regeneration of tendon bone interface |
| url | https://doi.org/10.1038/s41467-025-57499-x |
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