Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis
Abstract Background The purpose of our study was to explore the effect of nitrogen-containing bisphosphonate (N-BP) on vascular calcification (VC) through animal experiments and a meta-analysis. Methods In our animal experiments, Sprague-Dawley (SD) rats were randomly divided into a control group, a...
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2025-01-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-025-04526-w |
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| author | Wei Xu Guoyuan Lu Lifeng Gong Weigang Tang Wei Jiang |
| author_facet | Wei Xu Guoyuan Lu Lifeng Gong Weigang Tang Wei Jiang |
| author_sort | Wei Xu |
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| description | Abstract Background The purpose of our study was to explore the effect of nitrogen-containing bisphosphonate (N-BP) on vascular calcification (VC) through animal experiments and a meta-analysis. Methods In our animal experiments, Sprague-Dawley (SD) rats were randomly divided into a control group, a VC group, a low-dose zoledronic acid (ZOL) (20 µg/kg) group and a high-dose ZOL (100 µg/kg) group. The calcification of the aortic arch was observed by alizarin red staining. The calcium content of the aortic arch was measured. In our systematic review and meta-analysis, databases, including PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure (CNKI), and the Wanfang database, were searched from their inception to December 20, 2023. Eligible studies comparing N-BP versus no N-BP in the treatment of VC were included. Results In our animal experiment, the red-stained calcification structure in the low-dose ZOL group was slightly reduced and the red-stained calcification structure in the high-dose ZOL group was significantly reduced compared with that in the VC. The calcium content in the low-dose ZOL group was slightly lower than that in the VC group, but the difference was not significant (P = 0.08). The calcium content in the high-dose ZOL group was significantly lower than that in the VC group (P < 0.0001). Our meta-analysis of human studies revealed that N-BP did not reduce the arterial calcification score (P = 0.46). Our meta-analysis of animal studies revealed that N-BP did not significantly reduce the arterial calcification score (P = 0.09), but N-BP reduced the arterial calcification area (P < 0.00001), arterial calcium content (P = 0.009) and PO4 content (P = 0.0001). Conclusions Our animal experiment revealed that high-dose ZOL inhibited VC, but low-dose ZOL did not significantly inhibit VC. Our meta-analysis of human studies revealed that N-BP was not effective in the treatment of VC, but our meta-analysis of animal studies suggested a role of N-BP in inhibiting VC. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Cardiovascular Disorders |
| spelling | doaj-art-e908c6cbc74242e9be5d7544550338912025-02-02T12:07:37ZengBMCBMC Cardiovascular Disorders1471-22612025-01-012511910.1186/s12872-025-04526-wNitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysisWei Xu0Guoyuan Lu1Lifeng Gong2Weigang Tang3Wei Jiang4Department of Nephrology, The Wujin Clinical College of Xuzhou Medical UniversityDepartment of Nephrology, The First Affiliated Hospital of Soochow UniversityDepartment of Nephrology, The Wujin Clinical College of Xuzhou Medical UniversityDepartment of Nephrology, The Wujin Clinical College of Xuzhou Medical UniversityDepartment of Nephrology, The Wujin Clinical College of Xuzhou Medical UniversityAbstract Background The purpose of our study was to explore the effect of nitrogen-containing bisphosphonate (N-BP) on vascular calcification (VC) through animal experiments and a meta-analysis. Methods In our animal experiments, Sprague-Dawley (SD) rats were randomly divided into a control group, a VC group, a low-dose zoledronic acid (ZOL) (20 µg/kg) group and a high-dose ZOL (100 µg/kg) group. The calcification of the aortic arch was observed by alizarin red staining. The calcium content of the aortic arch was measured. In our systematic review and meta-analysis, databases, including PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure (CNKI), and the Wanfang database, were searched from their inception to December 20, 2023. Eligible studies comparing N-BP versus no N-BP in the treatment of VC were included. Results In our animal experiment, the red-stained calcification structure in the low-dose ZOL group was slightly reduced and the red-stained calcification structure in the high-dose ZOL group was significantly reduced compared with that in the VC. The calcium content in the low-dose ZOL group was slightly lower than that in the VC group, but the difference was not significant (P = 0.08). The calcium content in the high-dose ZOL group was significantly lower than that in the VC group (P < 0.0001). Our meta-analysis of human studies revealed that N-BP did not reduce the arterial calcification score (P = 0.46). Our meta-analysis of animal studies revealed that N-BP did not significantly reduce the arterial calcification score (P = 0.09), but N-BP reduced the arterial calcification area (P < 0.00001), arterial calcium content (P = 0.009) and PO4 content (P = 0.0001). Conclusions Our animal experiment revealed that high-dose ZOL inhibited VC, but low-dose ZOL did not significantly inhibit VC. Our meta-analysis of human studies revealed that N-BP was not effective in the treatment of VC, but our meta-analysis of animal studies suggested a role of N-BP in inhibiting VC.https://doi.org/10.1186/s12872-025-04526-wNitrogen-containing bisphosphonateVascular calcificationAnimal experimentsMeta-analysis |
| spellingShingle | Wei Xu Guoyuan Lu Lifeng Gong Weigang Tang Wei Jiang Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis BMC Cardiovascular Disorders Nitrogen-containing bisphosphonate Vascular calcification Animal experiments Meta-analysis |
| title | Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis |
| title_full | Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis |
| title_fullStr | Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis |
| title_full_unstemmed | Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis |
| title_short | Nitrogen-containing bisphosphonate for vascular calcification: animal experiments, systematic review and meta-analysis |
| title_sort | nitrogen containing bisphosphonate for vascular calcification animal experiments systematic review and meta analysis |
| topic | Nitrogen-containing bisphosphonate Vascular calcification Animal experiments Meta-analysis |
| url | https://doi.org/10.1186/s12872-025-04526-w |
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