A threshold equation for action potential initiation.

In central neurons, the threshold for spike initiation can depend on the stimulus and varies between cells and between recording sites in a given cell, but it is unclear what mechanisms underlie this variability. Properties of ionic channels are likely to play a role in threshold modulation. We exam...

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Main Authors: Jonathan Platkiewicz, Romain Brette
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-07-01
Series:PLoS Computational Biology
Online Access:https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1000850&type=printable
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author Jonathan Platkiewicz
Romain Brette
author_facet Jonathan Platkiewicz
Romain Brette
author_sort Jonathan Platkiewicz
collection DOAJ
description In central neurons, the threshold for spike initiation can depend on the stimulus and varies between cells and between recording sites in a given cell, but it is unclear what mechanisms underlie this variability. Properties of ionic channels are likely to play a role in threshold modulation. We examined in models the influence of Na channel activation, inactivation, slow voltage-gated channels and synaptic conductances on spike threshold. We propose a threshold equation which quantifies the contribution of all these mechanisms. It provides an instantaneous time-varying value of the threshold, which applies to neurons with fluctuating inputs. We deduce a differential equation for the threshold, similar to the equations of gating variables in the Hodgkin-Huxley formalism, which describes how the spike threshold varies with the membrane potential, depending on channel properties. We find that spike threshold depends logarithmically on Na channel density, and that Na channel inactivation and K channels can dynamically modulate it in an adaptive way: the threshold increases with membrane potential and after every action potential. Our equation was validated with simulations of a previously published multicompartemental model of spike initiation. Finally, we observed that threshold variability in models depends crucially on the shape of the Na activation function near spike initiation (about -55 mV), while its parameters are adjusted near half-activation voltage (about -30 mV), which might explain why many models exhibit little threshold variability, contrary to experimental observations. We conclude that ionic channels can account for large variations in spike threshold.
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spelling doaj-art-e8f8f5ff8b5f4d94bc8bab7f33d8471b2025-08-20T03:19:50ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582010-07-0167e100085010.1371/journal.pcbi.1000850A threshold equation for action potential initiation.Jonathan PlatkiewiczRomain BretteIn central neurons, the threshold for spike initiation can depend on the stimulus and varies between cells and between recording sites in a given cell, but it is unclear what mechanisms underlie this variability. Properties of ionic channels are likely to play a role in threshold modulation. We examined in models the influence of Na channel activation, inactivation, slow voltage-gated channels and synaptic conductances on spike threshold. We propose a threshold equation which quantifies the contribution of all these mechanisms. It provides an instantaneous time-varying value of the threshold, which applies to neurons with fluctuating inputs. We deduce a differential equation for the threshold, similar to the equations of gating variables in the Hodgkin-Huxley formalism, which describes how the spike threshold varies with the membrane potential, depending on channel properties. We find that spike threshold depends logarithmically on Na channel density, and that Na channel inactivation and K channels can dynamically modulate it in an adaptive way: the threshold increases with membrane potential and after every action potential. Our equation was validated with simulations of a previously published multicompartemental model of spike initiation. Finally, we observed that threshold variability in models depends crucially on the shape of the Na activation function near spike initiation (about -55 mV), while its parameters are adjusted near half-activation voltage (about -30 mV), which might explain why many models exhibit little threshold variability, contrary to experimental observations. We conclude that ionic channels can account for large variations in spike threshold.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1000850&type=printable
spellingShingle Jonathan Platkiewicz
Romain Brette
A threshold equation for action potential initiation.
PLoS Computational Biology
title A threshold equation for action potential initiation.
title_full A threshold equation for action potential initiation.
title_fullStr A threshold equation for action potential initiation.
title_full_unstemmed A threshold equation for action potential initiation.
title_short A threshold equation for action potential initiation.
title_sort threshold equation for action potential initiation
url https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1000850&type=printable
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