Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells
Scientists have tried to reprogram various origins of primary cells into human induced pluripotent stem cells (hiPSCs). Every somatic cell can theoretically become a hiPSC and give rise to targeted cells of the human body. However, there have been debates on the controversy about the differentiation...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/9432616 |
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| _version_ | 1832549409979629568 |
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| author | Yeri Alice Rim Yoojun Nam Narae Park Hyerin Jung Yeonsue Jang Jennifer Lee Ji Hyeon Ju |
| author_facet | Yeri Alice Rim Yoojun Nam Narae Park Hyerin Jung Yeonsue Jang Jennifer Lee Ji Hyeon Ju |
| author_sort | Yeri Alice Rim |
| collection | DOAJ |
| description | Scientists have tried to reprogram various origins of primary cells into human induced pluripotent stem cells (hiPSCs). Every somatic cell can theoretically become a hiPSC and give rise to targeted cells of the human body. However, there have been debates on the controversy about the differentiation propensity according to the origin of primary cells. We reprogrammed hiPSCs from four different types of primary cells such as dermal fibroblasts (DF, n=3), peripheral blood mononuclear cells (PBMC, n=3), cord blood mononuclear cells (CBMC, n=3), and osteoarthritis fibroblast-like synoviocytes (OAFLS, n=3). Established hiPSCs were differentiated into chondrogenic pellets. All told, cartilage-specific markers tended to express more by the order of CBMC > DF > PBMC > FLS. Origin of primary cells may influence the reprogramming and differentiation thereafter. In the context of chondrogenic propensity, CBMC-derived hiPSCs can be a fairly good candidate cell source for cartilage regeneration. The differentiation of hiPSCs into chondrocytes may help develop “cartilage in a dish” in the future. Also, the ideal cell source of hiPSC for chondrogenesis may contribute to future application as well. |
| format | Article |
| id | doaj-art-e8f5762a0c36487c94784fa2b056475b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e8f5762a0c36487c94784fa2b056475b2025-02-03T06:11:24ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/94326169432616Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary CellsYeri Alice Rim0Yoojun Nam1Narae Park2Hyerin Jung3Yeonsue Jang4Jennifer Lee5Ji Hyeon Ju6Catholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaCatholic iPSC Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaScientists have tried to reprogram various origins of primary cells into human induced pluripotent stem cells (hiPSCs). Every somatic cell can theoretically become a hiPSC and give rise to targeted cells of the human body. However, there have been debates on the controversy about the differentiation propensity according to the origin of primary cells. We reprogrammed hiPSCs from four different types of primary cells such as dermal fibroblasts (DF, n=3), peripheral blood mononuclear cells (PBMC, n=3), cord blood mononuclear cells (CBMC, n=3), and osteoarthritis fibroblast-like synoviocytes (OAFLS, n=3). Established hiPSCs were differentiated into chondrogenic pellets. All told, cartilage-specific markers tended to express more by the order of CBMC > DF > PBMC > FLS. Origin of primary cells may influence the reprogramming and differentiation thereafter. In the context of chondrogenic propensity, CBMC-derived hiPSCs can be a fairly good candidate cell source for cartilage regeneration. The differentiation of hiPSCs into chondrocytes may help develop “cartilage in a dish” in the future. Also, the ideal cell source of hiPSC for chondrogenesis may contribute to future application as well.http://dx.doi.org/10.1155/2018/9432616 |
| spellingShingle | Yeri Alice Rim Yoojun Nam Narae Park Hyerin Jung Yeonsue Jang Jennifer Lee Ji Hyeon Ju Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells Stem Cells International |
| title | Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells |
| title_full | Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells |
| title_fullStr | Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells |
| title_full_unstemmed | Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells |
| title_short | Different Chondrogenic Potential among Human Induced Pluripotent Stem Cells from Diverse Origin Primary Cells |
| title_sort | different chondrogenic potential among human induced pluripotent stem cells from diverse origin primary cells |
| url | http://dx.doi.org/10.1155/2018/9432616 |
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