Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis
Abstract Iron is an essential nutrient for all organisms. For pathogenic fungi, iron is essential for the success of infection. Thus, these organisms have developed high affinity iron uptake mechanisms to deal with metal deprivation imposed by the host. Siderophore production is one of the mechanism...
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BMC
2020-06-01
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| Series: | IMA Fungus |
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| Online Access: | http://link.springer.com/article/10.1186/s43008-020-00035-x |
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| author | Marielle Garcia Silva Juliana Santana de Curcio Mirelle Garcia Silva-Bailão Raisa Melo Lima Mariana Vieira Tomazett Aparecido Ferreira de Souza Vanessa Rafaela Milhomem Cruz-Leite Nicolau Sbaraini Alexandre Melo Bailão Fernando Rodrigues Maristela Pereira Relber Aguiar Gonçales Célia Maria de Almeida Soares |
| author_facet | Marielle Garcia Silva Juliana Santana de Curcio Mirelle Garcia Silva-Bailão Raisa Melo Lima Mariana Vieira Tomazett Aparecido Ferreira de Souza Vanessa Rafaela Milhomem Cruz-Leite Nicolau Sbaraini Alexandre Melo Bailão Fernando Rodrigues Maristela Pereira Relber Aguiar Gonçales Célia Maria de Almeida Soares |
| author_sort | Marielle Garcia Silva |
| collection | DOAJ |
| description | Abstract Iron is an essential nutrient for all organisms. For pathogenic fungi, iron is essential for the success of infection. Thus, these organisms have developed high affinity iron uptake mechanisms to deal with metal deprivation imposed by the host. Siderophore production is one of the mechanisms that fungal pathogens employ for iron acquisition. Paracoccidioides spp. present orthologous genes encoding the enzymes necessary for the biosynthesis of hydroxamates, and plasma membrane proteins related to the transport of these molecules. All these genes are induced in iron deprivation. In addition, it has been observed that Paracoccidioides spp. are able to use siderophores to scavenge iron. Here we observed that addition of the xenosiderophore ferrioxamine B FOB) to P. brasiliensis culture medium results in repression (at RNA and protein levels) of the SidA, the first enzyme of the siderophore biosynthesis pathway. Furthermore, SidA activity was reduced in the presence of FOB, suggesting that P. brasiliensis blocks siderophores biosynthesis and can explore siderophores in the environment to scavenge iron. In order to support the importance of siderophores on Paracoccidioides sp. life and infection cycle, silenced mutants for the sidA gene were obtained by antisense RNA technology. The obtained AsSidA strains displayed decreased siderophore biosynthesis in iron deprivation conditions and reduced virulence to an invertebrate model. |
| format | Article |
| id | doaj-art-e8f3f206e0384d3abbc3a9b2001eb57d |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-e8f3f206e0384d3abbc3a9b2001eb57d2025-02-02T18:45:39ZengBMCIMA Fungus2210-63592020-06-0111111410.1186/s43008-020-00035-xMolecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensisMarielle Garcia Silva0Juliana Santana de Curcio1Mirelle Garcia Silva-Bailão2Raisa Melo Lima3Mariana Vieira Tomazett4Aparecido Ferreira de Souza5Vanessa Rafaela Milhomem Cruz-Leite6Nicolau Sbaraini7Alexandre Melo Bailão8Fernando Rodrigues9Maristela Pereira10Relber Aguiar Gonçales11Célia Maria de Almeida Soares12Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásCentro de Biotecnologia, Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do SulLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLife and Health Sciences Research Institute (ICVS), School of Medicine, University of MinhoLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásLife and Health Sciences Research Institute (ICVS), School of Medicine, University of MinhoLaboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICB II, Campus II, Universidade Federal de GoiásAbstract Iron is an essential nutrient for all organisms. For pathogenic fungi, iron is essential for the success of infection. Thus, these organisms have developed high affinity iron uptake mechanisms to deal with metal deprivation imposed by the host. Siderophore production is one of the mechanisms that fungal pathogens employ for iron acquisition. Paracoccidioides spp. present orthologous genes encoding the enzymes necessary for the biosynthesis of hydroxamates, and plasma membrane proteins related to the transport of these molecules. All these genes are induced in iron deprivation. In addition, it has been observed that Paracoccidioides spp. are able to use siderophores to scavenge iron. Here we observed that addition of the xenosiderophore ferrioxamine B FOB) to P. brasiliensis culture medium results in repression (at RNA and protein levels) of the SidA, the first enzyme of the siderophore biosynthesis pathway. Furthermore, SidA activity was reduced in the presence of FOB, suggesting that P. brasiliensis blocks siderophores biosynthesis and can explore siderophores in the environment to scavenge iron. In order to support the importance of siderophores on Paracoccidioides sp. life and infection cycle, silenced mutants for the sidA gene were obtained by antisense RNA technology. The obtained AsSidA strains displayed decreased siderophore biosynthesis in iron deprivation conditions and reduced virulence to an invertebrate model.http://link.springer.com/article/10.1186/s43008-020-00035-xIronSidARNA interference |
| spellingShingle | Marielle Garcia Silva Juliana Santana de Curcio Mirelle Garcia Silva-Bailão Raisa Melo Lima Mariana Vieira Tomazett Aparecido Ferreira de Souza Vanessa Rafaela Milhomem Cruz-Leite Nicolau Sbaraini Alexandre Melo Bailão Fernando Rodrigues Maristela Pereira Relber Aguiar Gonçales Célia Maria de Almeida Soares Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis IMA Fungus Iron SidA RNA interference |
| title | Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis |
| title_full | Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis |
| title_fullStr | Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis |
| title_full_unstemmed | Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis |
| title_short | Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis |
| title_sort | molecular characterization of siderophore biosynthesis in paracoccidioides brasiliensis |
| topic | Iron SidA RNA interference |
| url | http://link.springer.com/article/10.1186/s43008-020-00035-x |
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