Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice
Recently, mRNA vaccines have been introduced as a safety-optimized alternative to plasmid DNA-based vaccines for protection against allergy. However, it remained unclear whether the short persistence of this vaccine type would limit memory responses and whether the protective immune response type wo...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/797421 |
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| _version_ | 1832559107217817600 |
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| author | E. Hattinger S. Scheiblhofer E. Roesler T. Thalhamer J. Thalhamer R. Weiss |
| author_facet | E. Hattinger S. Scheiblhofer E. Roesler T. Thalhamer J. Thalhamer R. Weiss |
| author_sort | E. Hattinger |
| collection | DOAJ |
| description | Recently, mRNA vaccines have been introduced as a safety-optimized alternative to plasmid DNA-based vaccines for protection against allergy. However, it remained unclear whether the short persistence of this vaccine type would limit memory responses and whether the protective immune response type would be maintained during recurrent exposure to allergen.
We tested the duration of protective memory responses in mice vaccinated with mRNA encoding the grass pollen allergen Phl p 5 by challenging them with recombinant allergen, 3.5, 6, and 9 months after vaccination. In a second experiment, vaccinated mice were repeatedly challenged monthly with aerosolized allergen over a period of 7 months. Antibody and cytokine responses as well as lung inflammation and airway hyperresponsiveness were assessed. mRNA vaccination induced robust TH1 memory responses for at least 9 months. Vaccination efficiently suppressed TH2 cytokines, IgE responses, and lung eosinophilia. Protection was maintained after repeated exposure to aerosolized allergen and no TH1 associated pathology was observed. Lung function remained improved compared to nonvaccinated controls. Our data clearly indicate that mRNA vaccination against Phl p 5 induces robust, long-lived memory responses, which can be recalled by allergen exposure without side effects. mRNA vaccines fulfill the requirements for safe prophylactic vaccination without the need for booster immunizations. |
| format | Article |
| id | doaj-art-e8e15552766045759cc337d34b6bedc0 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e8e15552766045759cc337d34b6bedc02025-02-03T01:30:52ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/797421797421Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in MiceE. Hattinger0S. Scheiblhofer1E. Roesler2T. Thalhamer3J. Thalhamer4R. Weiss5Department of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaDepartment of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaDepartment of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaDepartment of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaDepartment of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaDepartment of Molecular Biology, University of Salzburg, 5020 Salzburg, AustriaRecently, mRNA vaccines have been introduced as a safety-optimized alternative to plasmid DNA-based vaccines for protection against allergy. However, it remained unclear whether the short persistence of this vaccine type would limit memory responses and whether the protective immune response type would be maintained during recurrent exposure to allergen. We tested the duration of protective memory responses in mice vaccinated with mRNA encoding the grass pollen allergen Phl p 5 by challenging them with recombinant allergen, 3.5, 6, and 9 months after vaccination. In a second experiment, vaccinated mice were repeatedly challenged monthly with aerosolized allergen over a period of 7 months. Antibody and cytokine responses as well as lung inflammation and airway hyperresponsiveness were assessed. mRNA vaccination induced robust TH1 memory responses for at least 9 months. Vaccination efficiently suppressed TH2 cytokines, IgE responses, and lung eosinophilia. Protection was maintained after repeated exposure to aerosolized allergen and no TH1 associated pathology was observed. Lung function remained improved compared to nonvaccinated controls. Our data clearly indicate that mRNA vaccination against Phl p 5 induces robust, long-lived memory responses, which can be recalled by allergen exposure without side effects. mRNA vaccines fulfill the requirements for safe prophylactic vaccination without the need for booster immunizations.http://dx.doi.org/10.1155/2015/797421 |
| spellingShingle | E. Hattinger S. Scheiblhofer E. Roesler T. Thalhamer J. Thalhamer R. Weiss Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice Journal of Immunology Research |
| title | Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice |
| title_full | Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice |
| title_fullStr | Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice |
| title_full_unstemmed | Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice |
| title_short | Prophylactic mRNA Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice |
| title_sort | prophylactic mrna vaccination against allergy confers long term memory responses and persistent protection in mice |
| url | http://dx.doi.org/10.1155/2015/797421 |
| work_keys_str_mv | AT ehattinger prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice AT sscheiblhofer prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice AT eroesler prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice AT tthalhamer prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice AT jthalhamer prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice AT rweiss prophylacticmrnavaccinationagainstallergyconferslongtermmemoryresponsesandpersistentprotectioninmice |