Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance

The ovarian tumor microenvironment plays a critical yet is poorly understood role in the regulation of cancer cell behaviors including proliferation, migration, and response to chemotherapy treatments. Ovarian cancer is the deadliest gynecological cancer, due to diagnosis at late stages of the disea...

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Main Authors: Maranda Kramer, Allyson Criswell, Kamari Marzette, Emerson Cutcliffe, Mary Kathryn Sewell-Loftin
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Mechanobiology in Medicine
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2949907024000573
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author Maranda Kramer
Allyson Criswell
Kamari Marzette
Emerson Cutcliffe
Mary Kathryn Sewell-Loftin
author_facet Maranda Kramer
Allyson Criswell
Kamari Marzette
Emerson Cutcliffe
Mary Kathryn Sewell-Loftin
author_sort Maranda Kramer
collection DOAJ
description The ovarian tumor microenvironment plays a critical yet is poorly understood role in the regulation of cancer cell behaviors including proliferation, migration, and response to chemotherapy treatments. Ovarian cancer is the deadliest gynecological cancer, due to diagnosis at late stages of the disease and increased resistance to chemotherapies for recurrent disease. Understanding how the tumor microenvironment (TME) interacts with biomechanical forces to drive changes to ovarian cancer cell behaviors could elucidate novel treatment strategies for this patient population. Additionally, limitations in current preclinical models of the ovarian TME do not permit investigation of crosstalk between signaling pathways and mechanical forces. Our study focused on uncovering how strains and hyaluronic acid (HA) interact to signal through the CD44 receptor to alter ovarian cancer cell growth, migration, and response to a commonly used chemotherapy, paclitaxel. Using an advanced 3D in vitro model, we were able to identify how interactions of strain and HA as in the TME synergistically drive enhanced proliferation and migration in an ovarian tumor model line, while decreasing response to paclitaxel treatment. This study demonstrates the importance of elucidating how the mechanical forces present in the ovarian TME drive disease progression and response to treatment.
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spelling doaj-art-e8e0bced8fae407fa1ae7aaddbbfbba82025-08-20T02:30:23ZengElsevierMechanobiology in Medicine2949-90702024-12-012410009410.1016/j.mbm.2024.100094Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistanceMaranda Kramer0Allyson Criswell1Kamari Marzette2Emerson Cutcliffe3Mary Kathryn Sewell-Loftin4Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USABiomedical Sciences Program, Department of Clinical and Diagnostic Sciences, School of Health Professions, University of Alabama at Birmingham, USADepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA; O'Neal Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham AL 35233, USA; Corresponding author. Department of Biomedical Engineering, University of Alabama at Birmingham, Wallace Tumor Institute, 1824 6th Avenue South, Room 630A, Birmingham, AL 35294, USA.The ovarian tumor microenvironment plays a critical yet is poorly understood role in the regulation of cancer cell behaviors including proliferation, migration, and response to chemotherapy treatments. Ovarian cancer is the deadliest gynecological cancer, due to diagnosis at late stages of the disease and increased resistance to chemotherapies for recurrent disease. Understanding how the tumor microenvironment (TME) interacts with biomechanical forces to drive changes to ovarian cancer cell behaviors could elucidate novel treatment strategies for this patient population. Additionally, limitations in current preclinical models of the ovarian TME do not permit investigation of crosstalk between signaling pathways and mechanical forces. Our study focused on uncovering how strains and hyaluronic acid (HA) interact to signal through the CD44 receptor to alter ovarian cancer cell growth, migration, and response to a commonly used chemotherapy, paclitaxel. Using an advanced 3D in vitro model, we were able to identify how interactions of strain and HA as in the TME synergistically drive enhanced proliferation and migration in an ovarian tumor model line, while decreasing response to paclitaxel treatment. This study demonstrates the importance of elucidating how the mechanical forces present in the ovarian TME drive disease progression and response to treatment.http://www.sciencedirect.com/science/article/pii/S2949907024000573Ovarian cancerMechanobiologyTumor microenvironmentHyaluronic acidCD44Chemoresistance
spellingShingle Maranda Kramer
Allyson Criswell
Kamari Marzette
Emerson Cutcliffe
Mary Kathryn Sewell-Loftin
Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
Mechanobiology in Medicine
Ovarian cancer
Mechanobiology
Tumor microenvironment
Hyaluronic acid
CD44
Chemoresistance
title Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
title_full Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
title_fullStr Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
title_full_unstemmed Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
title_short Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance
title_sort strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation migration and drug resistance
topic Ovarian cancer
Mechanobiology
Tumor microenvironment
Hyaluronic acid
CD44
Chemoresistance
url http://www.sciencedirect.com/science/article/pii/S2949907024000573
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AT kamarimarzette strainandhyaluronicacidinteracttoregulateovariancancercellproliferationmigrationanddrugresistance
AT emersoncutcliffe strainandhyaluronicacidinteracttoregulateovariancancercellproliferationmigrationanddrugresistance
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