Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis
The study aims to investigate the effect of ginsenoside Rb1 on improving neutrophil extracellular traps-induced vascular endothelial damage. The CCK8 assay was used to evaluate the impact of ginsenoside Rb1 on cell activity. Flow cytometry was used to assess cell apoptosis. E-cadherin was used to d...
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Bangladesh Pharmacological Society
2024-12-01
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| Series: | Bangladesh Journal of Pharmacology |
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| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/76490 |
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| author | Biao Zhang Junkai Zhao Jiamin Yin Jiaping Chen Lingqi Xu |
| author_facet | Biao Zhang Junkai Zhao Jiamin Yin Jiaping Chen Lingqi Xu |
| author_sort | Biao Zhang |
| collection | DOAJ |
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The study aims to investigate the effect of ginsenoside Rb1 on improving neutrophil extracellular traps-induced vascular endothelial damage. The CCK8 assay was used to evaluate the impact of ginsenoside Rb1 on cell activity. Flow cytometry was used to assess cell apoptosis. E-cadherin was used to determine the damage to endothelial cells, and caspase 3 was used to detect apoptosis-related proteins. Ginsenoside Rb1 (100 μmol/L) could significantly increase the cell viability (p<0.05). At this concentration, the damage caused by neutrophil extracellular traps to human umbilical vein endothelial cells could be reversed (p<0.05). Ginsenoside Rb1 (100 μmol/L) could significantly inhibit the apoptosis and the expression of caspase3 protein caused by neutrophil extracellular traps (p<0.05). Ginsenoside Rb1 can reduce vascular endothelial injury induced by neutrophil extracellular traps, ginsenoside Rb1 protects vascular endothelium by inhibiting cell apoptosis.
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| format | Article |
| id | doaj-art-e8c815ccf68648b0ae3e60c0666fe676 |
| institution | Kabale University |
| issn | 1991-0088 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj-art-e8c815ccf68648b0ae3e60c0666fe6762025-02-02T13:09:57ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882024-12-0119310.3329/bjp.v19i3.76490Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosisBiao Zhang0https://orcid.org/0000-0002-0128-6149Junkai Zhao1Jiamin Yin2Jiaping Chen3Lingqi Xu4Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, ChinaDepartment of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Central Laboratory, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.Department of Emergency, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China. The study aims to investigate the effect of ginsenoside Rb1 on improving neutrophil extracellular traps-induced vascular endothelial damage. The CCK8 assay was used to evaluate the impact of ginsenoside Rb1 on cell activity. Flow cytometry was used to assess cell apoptosis. E-cadherin was used to determine the damage to endothelial cells, and caspase 3 was used to detect apoptosis-related proteins. Ginsenoside Rb1 (100 μmol/L) could significantly increase the cell viability (p<0.05). At this concentration, the damage caused by neutrophil extracellular traps to human umbilical vein endothelial cells could be reversed (p<0.05). Ginsenoside Rb1 (100 μmol/L) could significantly inhibit the apoptosis and the expression of caspase3 protein caused by neutrophil extracellular traps (p<0.05). Ginsenoside Rb1 can reduce vascular endothelial injury induced by neutrophil extracellular traps, ginsenoside Rb1 protects vascular endothelium by inhibiting cell apoptosis. https://www.banglajol.info/index.php/BJP/article/view/76490ApoptosisGinsenoside Rb1Immunofluorescence detectionNeutrophil extracellular traps Vascular endothelial injury |
| spellingShingle | Biao Zhang Junkai Zhao Jiamin Yin Jiaping Chen Lingqi Xu Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis Bangladesh Journal of Pharmacology Apoptosis Ginsenoside Rb1 Immunofluorescence detection Neutrophil extracellular traps Vascular endothelial injury |
| title | Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis |
| title_full | Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis |
| title_fullStr | Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis |
| title_full_unstemmed | Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis |
| title_short | Ginsenoside Rb1 ameliorates neutrophil extracellular traps-induced vascular endothelial damage by suppressing apoptosis |
| title_sort | ginsenoside rb1 ameliorates neutrophil extracellular traps induced vascular endothelial damage by suppressing apoptosis |
| topic | Apoptosis Ginsenoside Rb1 Immunofluorescence detection Neutrophil extracellular traps Vascular endothelial injury |
| url | https://www.banglajol.info/index.php/BJP/article/view/76490 |
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