Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development
Bifidobacterium species are essential members of the human gut microbiota, playing crucial roles in host health. Variations in the metabolic functions of different Bifidobacterium strains can have distinct health effects, making it essential to understand their metabolic characteristics for the deve...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1522036/full |
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| author | Kailong Liu Kailong Liu Kailong Liu Kailong Liu Guoqiang Yao Guoqiang Yao Guoqiang Yao Guoqiang Yao Zhan Yang Zhan Yang Zhan Yang Zhan Yang Tian Huang Tian Huang Tian Huang Tian Huang Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Heping Zhang Heping Zhang Heping Zhang Heping Zhang |
| author_facet | Kailong Liu Kailong Liu Kailong Liu Kailong Liu Guoqiang Yao Guoqiang Yao Guoqiang Yao Guoqiang Yao Zhan Yang Zhan Yang Zhan Yang Zhan Yang Tian Huang Tian Huang Tian Huang Tian Huang Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Heping Zhang Heping Zhang Heping Zhang Heping Zhang |
| author_sort | Kailong Liu |
| collection | DOAJ |
| description | Bifidobacterium species are essential members of the human gut microbiota, playing crucial roles in host health. Variations in the metabolic functions of different Bifidobacterium strains can have distinct health effects, making it essential to understand their metabolic characteristics for the development of targeted probiotic formulations. In this study, we cultivated four selected Bifidobacterium strains from three species: Bifidobacterium animalis subsp. lactis BB-69, Bbm-19, Bifidobacterium brevis BX-18, and Bifidobacterium longum subsp. infantis B8762. We conducted an untargeted comparative metabolomic analysis to investigate the intracellular metabolic profile and functional activities of these strains. A total of 1,340 metabolites were identified, revealing significant variations in their metabolomic profiles. Notably, Bifidobacterium animalis subsp. lactis Bbm-19 showed higher activity in amino acid biosynthesis, while Bifidobacterium animalis subsp. lactis BB-69 exhibited increased activity in secondary bile acid biosynthesis and alpha-linolenic acid metabolism. Bifidobacterium longum subsp. infantis B8762 demonstrated enhanced activity in polycyclic aromatic hydrocarbon degradation, vitamin digestion and absorption, and galactose metabolism. Bifidobacterium breve BX-18 was more active in tryptophan metabolism and pentose and glucuronate interconversions. Targeted analyses of specific metabolites further revealed strain-specific metabolic pathways. This study systematically elucidates the metabolic profile differences among the four investigated Bifidobacterium strains at the untargeted metabolomic level, providing valuable insights into their metabolic characteristics and potential applications in probiotic development. |
| format | Article |
| id | doaj-art-e8c24d5ca0654a8b8904a4a02fcafcbe |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e8c24d5ca0654a8b8904a4a02fcafcbe2025-01-31T11:21:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15220361522036Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic developmentKailong Liu0Kailong Liu1Kailong Liu2Kailong Liu3Guoqiang Yao4Guoqiang Yao5Guoqiang Yao6Guoqiang Yao7Zhan Yang8Zhan Yang9Zhan Yang10Zhan Yang11Tian Huang12Tian Huang13Tian Huang14Tian Huang15Lai-Yu Kwok16Lai-Yu Kwok17Lai-Yu Kwok18Lai-Yu Kwok19Heping Zhang20Heping Zhang21Heping Zhang22Heping Zhang23Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, ChinaInner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, ChinaCollaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, ChinaBifidobacterium species are essential members of the human gut microbiota, playing crucial roles in host health. Variations in the metabolic functions of different Bifidobacterium strains can have distinct health effects, making it essential to understand their metabolic characteristics for the development of targeted probiotic formulations. In this study, we cultivated four selected Bifidobacterium strains from three species: Bifidobacterium animalis subsp. lactis BB-69, Bbm-19, Bifidobacterium brevis BX-18, and Bifidobacterium longum subsp. infantis B8762. We conducted an untargeted comparative metabolomic analysis to investigate the intracellular metabolic profile and functional activities of these strains. A total of 1,340 metabolites were identified, revealing significant variations in their metabolomic profiles. Notably, Bifidobacterium animalis subsp. lactis Bbm-19 showed higher activity in amino acid biosynthesis, while Bifidobacterium animalis subsp. lactis BB-69 exhibited increased activity in secondary bile acid biosynthesis and alpha-linolenic acid metabolism. Bifidobacterium longum subsp. infantis B8762 demonstrated enhanced activity in polycyclic aromatic hydrocarbon degradation, vitamin digestion and absorption, and galactose metabolism. Bifidobacterium breve BX-18 was more active in tryptophan metabolism and pentose and glucuronate interconversions. Targeted analyses of specific metabolites further revealed strain-specific metabolic pathways. This study systematically elucidates the metabolic profile differences among the four investigated Bifidobacterium strains at the untargeted metabolomic level, providing valuable insights into their metabolic characteristics and potential applications in probiotic development.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1522036/fullBifidobacterium animalis subsp. lactisBifidobacterium longum subsp. infantisBifidobacterium brevebioactive compoundsuntargeted metabolomic |
| spellingShingle | Kailong Liu Kailong Liu Kailong Liu Kailong Liu Guoqiang Yao Guoqiang Yao Guoqiang Yao Guoqiang Yao Zhan Yang Zhan Yang Zhan Yang Zhan Yang Tian Huang Tian Huang Tian Huang Tian Huang Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Lai-Yu Kwok Heping Zhang Heping Zhang Heping Zhang Heping Zhang Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development Frontiers in Microbiology Bifidobacterium animalis subsp. lactis Bifidobacterium longum subsp. infantis Bifidobacterium breve bioactive compounds untargeted metabolomic |
| title | Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development |
| title_full | Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development |
| title_fullStr | Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development |
| title_full_unstemmed | Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development |
| title_short | Untargeted metabolomic analysis uncovers metabolic variability of four Bifidobacterial strains for probiotic development |
| title_sort | untargeted metabolomic analysis uncovers metabolic variability of four bifidobacterial strains for probiotic development |
| topic | Bifidobacterium animalis subsp. lactis Bifidobacterium longum subsp. infantis Bifidobacterium breve bioactive compounds untargeted metabolomic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1522036/full |
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