The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin
ABSTRACT The symbiotic relationship between rhizobia and legumes is critical for sustainable agriculture and has important economic and environmental implications. In this intricate process, rhizobial bacteria colonise plant roots and induce the formation of specialised plant organs, the nodules. Wi...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-03-01
|
| Series: | Microbial Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1751-7915.70112 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850090439474937856 |
|---|---|
| author | Pedro José Reyes‐Pérez Irene Jiménez‐Guerrero Ana Sánchez‐Reina Cristina Civantos Natalia Moreno‐de Castro Francisco Javier Ollero Jacinto Gandullo Patricia Bernal Francisco Pérez‐Montaño |
| author_facet | Pedro José Reyes‐Pérez Irene Jiménez‐Guerrero Ana Sánchez‐Reina Cristina Civantos Natalia Moreno‐de Castro Francisco Javier Ollero Jacinto Gandullo Patricia Bernal Francisco Pérez‐Montaño |
| author_sort | Pedro José Reyes‐Pérez |
| collection | DOAJ |
| description | ABSTRACT The symbiotic relationship between rhizobia and legumes is critical for sustainable agriculture and has important economic and environmental implications. In this intricate process, rhizobial bacteria colonise plant roots and induce the formation of specialised plant organs, the nodules. Within these structures, rhizobia fix environmental nitrogen into ammonia, significantly reducing the demand for synthetic fertilisers. Multiple bacterial secretion systems (TXSS, Type X Secretion System) are involved in establishing this symbiosis, with T3SS being the most studied. While the Type 6 Secretion System (T6SS) is known as a “nanoweapon” commonly used by diderm (formerly gram‐negative) bacteria for inter‐bacterial competition and potentially manipulating eukaryotic cells, its precise role in legume symbiosis remains unclear. Sinorhizobium fredii USDA257, a fast‐growing rhizobial strain capable of nodulating diverse legume plants, possesses a single T6SS cluster containing genes encoding structural components and potential effectors that could target plant cells and/or act as effector‐immunity pairs. Our research reveals that this T6SS can be induced in nutrient‐limited conditions and, more importantly, is essential for successful nodulation and competitive colonisation of Glycine max cv Pekin. Although the system did not demonstrate effectiveness in eliminating competing bacteria in vitro, its active presence within root nodules suggests a sophisticated role in symbiotic interactions that extends beyond traditional interbacterial competition. |
| format | Article |
| id | doaj-art-e8a5ce7ee33a47eb843e613428f0cbba |
| institution | DOAJ |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj-art-e8a5ce7ee33a47eb843e613428f0cbba2025-08-20T02:42:34ZengWileyMicrobial Biotechnology1751-79152025-03-01183n/an/a10.1111/1751-7915.70112The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv PekinPedro José Reyes‐Pérez0Irene Jiménez‐Guerrero1Ana Sánchez‐Reina2Cristina Civantos3Natalia Moreno‐de Castro4Francisco Javier Ollero5Jacinto Gandullo6Patricia Bernal7Francisco Pérez‐Montaño8Departamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Biología Vegetal y Ecología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainDepartamento de Microbiología, Facultad de Biología Universidad de Sevilla Sevilla SpainABSTRACT The symbiotic relationship between rhizobia and legumes is critical for sustainable agriculture and has important economic and environmental implications. In this intricate process, rhizobial bacteria colonise plant roots and induce the formation of specialised plant organs, the nodules. Within these structures, rhizobia fix environmental nitrogen into ammonia, significantly reducing the demand for synthetic fertilisers. Multiple bacterial secretion systems (TXSS, Type X Secretion System) are involved in establishing this symbiosis, with T3SS being the most studied. While the Type 6 Secretion System (T6SS) is known as a “nanoweapon” commonly used by diderm (formerly gram‐negative) bacteria for inter‐bacterial competition and potentially manipulating eukaryotic cells, its precise role in legume symbiosis remains unclear. Sinorhizobium fredii USDA257, a fast‐growing rhizobial strain capable of nodulating diverse legume plants, possesses a single T6SS cluster containing genes encoding structural components and potential effectors that could target plant cells and/or act as effector‐immunity pairs. Our research reveals that this T6SS can be induced in nutrient‐limited conditions and, more importantly, is essential for successful nodulation and competitive colonisation of Glycine max cv Pekin. Although the system did not demonstrate effectiveness in eliminating competing bacteria in vitro, its active presence within root nodules suggests a sophisticated role in symbiotic interactions that extends beyond traditional interbacterial competition.https://doi.org/10.1111/1751-7915.70112competitionlegumenodulationrhizobiumsymbiosisT6SS effectors |
| spellingShingle | Pedro José Reyes‐Pérez Irene Jiménez‐Guerrero Ana Sánchez‐Reina Cristina Civantos Natalia Moreno‐de Castro Francisco Javier Ollero Jacinto Gandullo Patricia Bernal Francisco Pérez‐Montaño The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin Microbial Biotechnology competition legume nodulation rhizobium symbiosis T6SS effectors |
| title | The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin |
| title_full | The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin |
| title_fullStr | The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin |
| title_full_unstemmed | The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin |
| title_short | The Type VI Secretion System of Sinorhizobium fredii USDA257 Is Required for Successful Nodulation With Glycine max cv Pekin |
| title_sort | type vi secretion system of sinorhizobium fredii usda257 is required for successful nodulation with glycine max cv pekin |
| topic | competition legume nodulation rhizobium symbiosis T6SS effectors |
| url | https://doi.org/10.1111/1751-7915.70112 |
| work_keys_str_mv | AT pedrojosereyesperez thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT irenejimenezguerrero thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT anasanchezreina thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT cristinacivantos thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT nataliamorenodecastro thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT franciscojavierollero thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT jacintogandullo thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT patriciabernal thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT franciscoperezmontano thetypevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT pedrojosereyesperez typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT irenejimenezguerrero typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT anasanchezreina typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT cristinacivantos typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT nataliamorenodecastro typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT franciscojavierollero typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT jacintogandullo typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT patriciabernal typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin AT franciscoperezmontano typevisecretionsystemofsinorhizobiumfrediiusda257isrequiredforsuccessfulnodulationwithglycinemaxcvpekin |