Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway
Largemouth bass have been plagued by diseases elicited by bacteria, parasites, and viruses, particularly viral diseases, which have caused significant economic losses in recent years. And there is a lack of approved antiviral drugs in the aquaculture industry. Micropterus salmoides Rhabdovirus (MSRV...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X23001053 |
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| author | Zhiwen Wang Ping Deng Zemao Gu Lijuan Li Junfa Yuan |
| author_facet | Zhiwen Wang Ping Deng Zemao Gu Lijuan Li Junfa Yuan |
| author_sort | Zhiwen Wang |
| collection | DOAJ |
| description | Largemouth bass have been plagued by diseases elicited by bacteria, parasites, and viruses, particularly viral diseases, which have caused significant economic losses in recent years. And there is a lack of approved antiviral drugs in the aquaculture industry. Micropterus salmoides Rhabdovirus (MSRV) is the causative agent of Micropterus salmoides Rhabdovirus disease, which mostly affects largemouth bass fry. By using experimental infection, transcriptome sequencing, and inhibitor treatment, the inflammatory response mediated by mitogen-activated protein kinase (MAPK), phosphatidylinositol3-kinase (PI3K), and nuclear factor kappa-B (NF-κB) pathways was verified as one of the pathological processes of MSRV infection. Further, Myricetin, arteannuin, naringenin, ursolic acid, andrographolide, and resveratrol were selected to evolute their anti-inflammatory activity based on the promoter of NF-κB and found myricetin, naringenin, andrographolide, and resveratrol significantly inhibited NF-κB 1 promoter activity. Myricetin was found to damp MSRV caused-inflammation and inhibit MSRV infection by suppressing NF-κB signaling. Besides, myricetin improves the survival rate of largemouth bass after MSRV infection. In conclusion, myricetin presented anti-MSRV activity by targeting the NF-κB inflammatory pathway and can be further developed as a potential preventative drug of rhabdovirus disease. |
| format | Article |
| id | doaj-art-e897a2b25469409eac9e2400e2dbcd86 |
| institution | Kabale University |
| issn | 2468-550X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj-art-e897a2b25469409eac9e2400e2dbcd862025-08-20T03:42:15ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-03-0110227128010.1016/j.aaf.2023.08.004Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathwayZhiwen Wang0Ping Deng1Zemao Gu2Lijuan Li3Junfa Yuan4Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; National Aquatic Animal Diseases Para-reference Laboratory (HZAU), Wuhan, 430070, ChinaWuhan Academy of Agricultural Sciences, Wuhan, 430070, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; National Aquatic Animal Diseases Para-reference Laboratory (HZAU), Wuhan, 430070, China; Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; National Aquatic Animal Diseases Para-reference Laboratory (HZAU), Wuhan, 430070, China; Hubei Engineering Research Center for Aquatic Animal Diseases Control and Prevention, Wuhan, 430070, China; Corresponding author. Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China.Largemouth bass have been plagued by diseases elicited by bacteria, parasites, and viruses, particularly viral diseases, which have caused significant economic losses in recent years. And there is a lack of approved antiviral drugs in the aquaculture industry. Micropterus salmoides Rhabdovirus (MSRV) is the causative agent of Micropterus salmoides Rhabdovirus disease, which mostly affects largemouth bass fry. By using experimental infection, transcriptome sequencing, and inhibitor treatment, the inflammatory response mediated by mitogen-activated protein kinase (MAPK), phosphatidylinositol3-kinase (PI3K), and nuclear factor kappa-B (NF-κB) pathways was verified as one of the pathological processes of MSRV infection. Further, Myricetin, arteannuin, naringenin, ursolic acid, andrographolide, and resveratrol were selected to evolute their anti-inflammatory activity based on the promoter of NF-κB and found myricetin, naringenin, andrographolide, and resveratrol significantly inhibited NF-κB 1 promoter activity. Myricetin was found to damp MSRV caused-inflammation and inhibit MSRV infection by suppressing NF-κB signaling. Besides, myricetin improves the survival rate of largemouth bass after MSRV infection. In conclusion, myricetin presented anti-MSRV activity by targeting the NF-κB inflammatory pathway and can be further developed as a potential preventative drug of rhabdovirus disease.http://www.sciencedirect.com/science/article/pii/S2468550X23001053Micropterus salmoides rhabdovirusInflammationAntiviral drugsMyricetinNuclear factor kappa-B |
| spellingShingle | Zhiwen Wang Ping Deng Zemao Gu Lijuan Li Junfa Yuan Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway Aquaculture and Fisheries Micropterus salmoides rhabdovirus Inflammation Antiviral drugs Myricetin Nuclear factor kappa-B |
| title | Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway |
| title_full | Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway |
| title_fullStr | Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway |
| title_full_unstemmed | Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway |
| title_short | Myricetin inhibits Micropterus salmoides rhabdovirus infection by damping inflammation through the NF-κB pathway |
| title_sort | myricetin inhibits micropterus salmoides rhabdovirus infection by damping inflammation through the nf κb pathway |
| topic | Micropterus salmoides rhabdovirus Inflammation Antiviral drugs Myricetin Nuclear factor kappa-B |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X23001053 |
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