Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity
The objective of this study was to determine whether degradation products from spongy gelatin scaffolds can enhance angiogenesis and orthotopic bone regeneration. Spongy gelatin disks were prepared using gelatin solution concentrations ranging from 1% to 7% (v/w) within cylindrical tubes through pro...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Science and Technology of Advanced Materials |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14686996.2023.2277675 |
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| author | Takanori Hatakeyama Ryo Hamai Yukari Shiwaku Takahisa Anada Susumu Sakai Tomoya Sato Kazuyoshi Baba Keiichi Sasaki Osamu Suzuki |
| author_facet | Takanori Hatakeyama Ryo Hamai Yukari Shiwaku Takahisa Anada Susumu Sakai Tomoya Sato Kazuyoshi Baba Keiichi Sasaki Osamu Suzuki |
| author_sort | Takanori Hatakeyama |
| collection | DOAJ |
| description | The objective of this study was to determine whether degradation products from spongy gelatin scaffolds can enhance angiogenesis and orthotopic bone regeneration. Spongy gelatin disks were prepared using gelatin solution concentrations ranging from 1% to 7% (v/w) within cylindrical tubes through programmed freezing, lyophilization, cutting, and dehydrothermal crosslinking and implanted in critical-sized defects of rat calvaria for up to 8 weeks. Analyses of disk implantation into rat calvaria defects by microfocus X-ray computed tomography and histomorphometry indicated that the bone volume was significantly larger in the 5% and 7% gelatin sponge groups than in the 1% and 3% gelatin sponge groups and tended to increase progressively from 5% to 7%. The histomorphometric analysis also showed that the largest number of new vessels was formed in the defect treated with 5% gelatin sponge compared to other gelatin sponges. Immunohistochemistry of matrix metalloproteinase (MMP) indicated that the gelatin concentration in the disks affected the appearance of MMP-2 and MMP-9 positive cells around the skeleton of the gelatin sponges. The degradation products of gelatin by MMP-9 and prolyl endopeptidase enhanced the formation of a capillary-like structure in human umbilical vein endothelial cells in vitro. These results suggested that the higher-density gelatin sponges tended to supply their own molecules via biodegradation, resulting in enhanced orthotopic osteogenesis through the expected function of gelatin molecules in angiogenesis and bone formation. |
| format | Article |
| id | doaj-art-e87da3257dca400abfa7e941d2fcbab6 |
| institution | DOAJ |
| issn | 1468-6996 1878-5514 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj-art-e87da3257dca400abfa7e941d2fcbab62025-08-20T02:42:51ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142023-12-0124110.1080/14686996.2023.2277675Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacityTakanori Hatakeyama0Ryo Hamai1Yukari Shiwaku2Takahisa Anada3Susumu Sakai4Tomoya Sato5Kazuyoshi Baba6Keiichi Sasaki7Osamu Suzuki8Division of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanDivision of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, JapanDepartment of Orthopedic Surgery, Tohoku University Graduate School of Medicine, Sendai, JapanTohoku University, Sendai, JapanDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, Sendai, JapanThe objective of this study was to determine whether degradation products from spongy gelatin scaffolds can enhance angiogenesis and orthotopic bone regeneration. Spongy gelatin disks were prepared using gelatin solution concentrations ranging from 1% to 7% (v/w) within cylindrical tubes through programmed freezing, lyophilization, cutting, and dehydrothermal crosslinking and implanted in critical-sized defects of rat calvaria for up to 8 weeks. Analyses of disk implantation into rat calvaria defects by microfocus X-ray computed tomography and histomorphometry indicated that the bone volume was significantly larger in the 5% and 7% gelatin sponge groups than in the 1% and 3% gelatin sponge groups and tended to increase progressively from 5% to 7%. The histomorphometric analysis also showed that the largest number of new vessels was formed in the defect treated with 5% gelatin sponge compared to other gelatin sponges. Immunohistochemistry of matrix metalloproteinase (MMP) indicated that the gelatin concentration in the disks affected the appearance of MMP-2 and MMP-9 positive cells around the skeleton of the gelatin sponges. The degradation products of gelatin by MMP-9 and prolyl endopeptidase enhanced the formation of a capillary-like structure in human umbilical vein endothelial cells in vitro. These results suggested that the higher-density gelatin sponges tended to supply their own molecules via biodegradation, resulting in enhanced orthotopic osteogenesis through the expected function of gelatin molecules in angiogenesis and bone formation.https://www.tandfonline.com/doi/10.1080/14686996.2023.2277675Gelatinscaffolddegradation productsangiogenesisbone regeneration |
| spellingShingle | Takanori Hatakeyama Ryo Hamai Yukari Shiwaku Takahisa Anada Susumu Sakai Tomoya Sato Kazuyoshi Baba Keiichi Sasaki Osamu Suzuki Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity Science and Technology of Advanced Materials Gelatin scaffold degradation products angiogenesis bone regeneration |
| title | Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity |
| title_full | Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity |
| title_fullStr | Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity |
| title_full_unstemmed | Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity |
| title_short | Effects of degradation products from gelatin spongy scaffolds on angio-osteogenic capacity |
| title_sort | effects of degradation products from gelatin spongy scaffolds on angio osteogenic capacity |
| topic | Gelatin scaffold degradation products angiogenesis bone regeneration |
| url | https://www.tandfonline.com/doi/10.1080/14686996.2023.2277675 |
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