Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh
Abstract Background The emergence and dissemination of multidrug resistant (MDR) bacteria pose a severe threat to public health by limiting clinical treatment and prophylactic options. Objectives This study investigates the prevalence of Escherichia coli in broilers, their phenotypic antimicrobial r...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-11-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70032 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593442401681408 |
|---|---|
| author | Md. Sirazul Islam Chandan Nath F. M. Yasir Hasib Tahia Ahmed Logno Md. Helal Uddin Mohammad Mahmudul Hassan Sharmin Chowdhury |
| author_facet | Md. Sirazul Islam Chandan Nath F. M. Yasir Hasib Tahia Ahmed Logno Md. Helal Uddin Mohammad Mahmudul Hassan Sharmin Chowdhury |
| author_sort | Md. Sirazul Islam |
| collection | DOAJ |
| description | Abstract Background The emergence and dissemination of multidrug resistant (MDR) bacteria pose a severe threat to public health by limiting clinical treatment and prophylactic options. Objectives This study investigates the prevalence of Escherichia coli in broilers, their phenotypic antimicrobial resistance (AMR) profiles and the presence of virulence‐associated genes (VAGs) and antimicrobial resistance genes (ARGs) using polymerase chain reaction (PCR). Materials and methods A total of 216 pooled cloacal samples were collected from 1080 broilers across six districts of Bangladesh. Each pooled sample comprised randomly selected cloacal swabs from five birds per farm. E. coli isolates were identified using standard bacteriological approach, followed by biochemical assays and PCR. Antimicrobial susceptibility was assessed using the Kirby–Bauer disc diffusion method, and the presence of ARGs and VAGs was determined via PCR. Five selected isolates were partially sequenced for five VAGs using Sanger sequencing. Results A total of 177 E. coli isolates (81.94%, 95% confidence interval: 76.24%–86.53%) were identified. The isolates showed the highest resistance to ampicillin (93.79%), followed by tetracycline (91.53%), erythromycin (89.27%) and ciprofloxacin (87%). Conversely, ceftriaxone (80.79%) showed highest susceptibility, followed by gentamicin (37.29%) and neomycin (31.07%). All isolates were MDR, with a multiple antibiotic resistance indexes were <0.3. A significant percentage (16.38%) of E. coli isolates were MDR to five antimicrobial classes and harboured blaTEM, sul1, ere (A), tetA, tetB and tetC genes. The highest prevalent ARGs were blaTEM (88.14%) followed by ere (A) (83.62%) and sul 1 (72.32%). The prevalence of VAGs was astA (56.50%), iucD (31.07%), iss (21.47%), irp2 (15.82%) and cva/cvi (3.39%), respectively. Conclusions This study highlights the presence of ARGs contributing to the development of MDR in E. coli carrying VAGs in broilers. Effective monitoring and surveillance of antimicrobial usage in poultry production systems are urgently required to prevent emergence and dissemination of AMR. |
| format | Article |
| id | doaj-art-e87b6611bb3140d687e009cf3a1549f3 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-e87b6611bb3140d687e009cf3a1549f32025-01-20T17:16:44ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70032Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in BangladeshMd. Sirazul Islam0Chandan Nath1F. M. Yasir Hasib2Tahia Ahmed Logno3Md. Helal Uddin4Mohammad Mahmudul Hassan5Sharmin Chowdhury6Department of Pathology and ParasitologyFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshDepartment of Microbiology and Veterinary Public HealthFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshDepartment of Pathology and ParasitologyFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshDepartment of Microbiology and Veterinary Public HealthFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshDepartment of Medicine and SurgeryFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshQueensland Alliance for One Health SciencesSchool of Veterinary ScienceThe University of QueenslandGattonQueenslandAustraliaDepartment of Pathology and ParasitologyFaculty of Veterinary MedicineChattogram Veterinary and Animal Sciences UniversityChattogramBangladeshAbstract Background The emergence and dissemination of multidrug resistant (MDR) bacteria pose a severe threat to public health by limiting clinical treatment and prophylactic options. Objectives This study investigates the prevalence of Escherichia coli in broilers, their phenotypic antimicrobial resistance (AMR) profiles and the presence of virulence‐associated genes (VAGs) and antimicrobial resistance genes (ARGs) using polymerase chain reaction (PCR). Materials and methods A total of 216 pooled cloacal samples were collected from 1080 broilers across six districts of Bangladesh. Each pooled sample comprised randomly selected cloacal swabs from five birds per farm. E. coli isolates were identified using standard bacteriological approach, followed by biochemical assays and PCR. Antimicrobial susceptibility was assessed using the Kirby–Bauer disc diffusion method, and the presence of ARGs and VAGs was determined via PCR. Five selected isolates were partially sequenced for five VAGs using Sanger sequencing. Results A total of 177 E. coli isolates (81.94%, 95% confidence interval: 76.24%–86.53%) were identified. The isolates showed the highest resistance to ampicillin (93.79%), followed by tetracycline (91.53%), erythromycin (89.27%) and ciprofloxacin (87%). Conversely, ceftriaxone (80.79%) showed highest susceptibility, followed by gentamicin (37.29%) and neomycin (31.07%). All isolates were MDR, with a multiple antibiotic resistance indexes were <0.3. A significant percentage (16.38%) of E. coli isolates were MDR to five antimicrobial classes and harboured blaTEM, sul1, ere (A), tetA, tetB and tetC genes. The highest prevalent ARGs were blaTEM (88.14%) followed by ere (A) (83.62%) and sul 1 (72.32%). The prevalence of VAGs was astA (56.50%), iucD (31.07%), iss (21.47%), irp2 (15.82%) and cva/cvi (3.39%), respectively. Conclusions This study highlights the presence of ARGs contributing to the development of MDR in E. coli carrying VAGs in broilers. Effective monitoring and surveillance of antimicrobial usage in poultry production systems are urgently required to prevent emergence and dissemination of AMR.https://doi.org/10.1002/vms3.70032AMRARGschickenpublic healthVAGs |
| spellingShingle | Md. Sirazul Islam Chandan Nath F. M. Yasir Hasib Tahia Ahmed Logno Md. Helal Uddin Mohammad Mahmudul Hassan Sharmin Chowdhury Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh Veterinary Medicine and Science AMR ARGs chicken public health VAGs |
| title | Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh |
| title_full | Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh |
| title_fullStr | Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh |
| title_full_unstemmed | Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh |
| title_short | Detection and characterization of multidrug resistant Escherichia coli carrying virulence gene isolated from broilers in Bangladesh |
| title_sort | detection and characterization of multidrug resistant escherichia coli carrying virulence gene isolated from broilers in bangladesh |
| topic | AMR ARGs chicken public health VAGs |
| url | https://doi.org/10.1002/vms3.70032 |
| work_keys_str_mv | AT mdsirazulislam detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT chandannath detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT fmyasirhasib detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT tahiaahmedlogno detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT mdhelaluddin detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT mohammadmahmudulhassan detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh AT sharminchowdhury detectionandcharacterizationofmultidrugresistantescherichiacolicarryingvirulencegeneisolatedfrombroilersinbangladesh |