Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers
Abstract Background Residual root canal microbiota in the inaccessible areas of the root canal despite cleaning and shaping and the adverse effects of the various root canal irrigants on dentin mineral content pose a major challenge to successful root canal therapy. The aim of this study was to deve...
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2025-07-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-06469-2 |
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| author | Samar M. Nashaat Enas T. Enan Ahmed S.G. Srag El-Din Dina Abdelaziz |
| author_facet | Samar M. Nashaat Enas T. Enan Ahmed S.G. Srag El-Din Dina Abdelaziz |
| author_sort | Samar M. Nashaat |
| collection | DOAJ |
| description | Abstract Background Residual root canal microbiota in the inaccessible areas of the root canal despite cleaning and shaping and the adverse effects of the various root canal irrigants on dentin mineral content pose a major challenge to successful root canal therapy. The aim of this study was to develop therapeutic and bioactive resin-based root canal sealer (RCS) with good wetting ability. Methods Silver polydopamine-modified hydroxyapatite (HA-PDA-Ag-PDA) fillers were synthesized. Hydroxyapatite particles were analyzed using X-ray diffraction (XRD), while the final prepared fillers were characterized by Fourier transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). The resin-based RCS (Adseal®) served as the control group (G0). Two concentrations of HA-PDA-Ag-PDA fillers (1 wt% and 2 wt%) were incorporated into the tested RCS (G1 and G2), respectively. The antibacterial properties, mineralization, and wettability of unmodified RCS (G0) and modified RCS (G1 and G2) were evaluated. The data were analyzed by one-way ANOVA, followed by a post hoc test for pairwise comparisons. Results XRD, FT-IR, TEM characterizations confirmed the successful synthesis of the HA-PDA-Ag-PDA fillers. Modified RCS showed strong bacterial inhibition effect against E. faecalis with insignificant difference between G1 and G2. Modified RCS (G1 and G2) also showed good mineralization potential and improvement in wettability of dentin surface. Conclusions HA-PDA-Ag-PDA fillers significantly improved the antibacterial, mineralization properties as well as the wetting ability of resin-based RCS. |
| format | Article |
| id | doaj-art-e87a90860b5045518c87a40c6f0172ab |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-e87a90860b5045518c87a40c6f0172ab2025-08-20T04:02:41ZengBMCBMC Oral Health1472-68312025-07-0125111210.1186/s12903-025-06469-2Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillersSamar M. Nashaat0Enas T. Enan1Ahmed S.G. Srag El-Din2Dina Abdelaziz3Department of Dental Biomaterials, Faculty of Dentistry, Mansoura UniversityDepartment of Dental Biomaterials, Faculty of Dentistry, Mansoura UniversityDepartment of Pharmaceutics, College of Pharmacy, Almaaqal UniversityDepartment of Dental Biomaterials, Faculty of Dentistry, Mansoura UniversityAbstract Background Residual root canal microbiota in the inaccessible areas of the root canal despite cleaning and shaping and the adverse effects of the various root canal irrigants on dentin mineral content pose a major challenge to successful root canal therapy. The aim of this study was to develop therapeutic and bioactive resin-based root canal sealer (RCS) with good wetting ability. Methods Silver polydopamine-modified hydroxyapatite (HA-PDA-Ag-PDA) fillers were synthesized. Hydroxyapatite particles were analyzed using X-ray diffraction (XRD), while the final prepared fillers were characterized by Fourier transform infrared (FT-IR) spectroscopy and transmission electron microscopy (TEM). The resin-based RCS (Adseal®) served as the control group (G0). Two concentrations of HA-PDA-Ag-PDA fillers (1 wt% and 2 wt%) were incorporated into the tested RCS (G1 and G2), respectively. The antibacterial properties, mineralization, and wettability of unmodified RCS (G0) and modified RCS (G1 and G2) were evaluated. The data were analyzed by one-way ANOVA, followed by a post hoc test for pairwise comparisons. Results XRD, FT-IR, TEM characterizations confirmed the successful synthesis of the HA-PDA-Ag-PDA fillers. Modified RCS showed strong bacterial inhibition effect against E. faecalis with insignificant difference between G1 and G2. Modified RCS (G1 and G2) also showed good mineralization potential and improvement in wettability of dentin surface. Conclusions HA-PDA-Ag-PDA fillers significantly improved the antibacterial, mineralization properties as well as the wetting ability of resin-based RCS.https://doi.org/10.1186/s12903-025-06469-2AntibacterialHydroxyapatitePolydopamineRemineralizationRoot canal sealerSilver nanoparticles |
| spellingShingle | Samar M. Nashaat Enas T. Enan Ahmed S.G. Srag El-Din Dina Abdelaziz Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers BMC Oral Health Antibacterial Hydroxyapatite Polydopamine Remineralization Root canal sealer Silver nanoparticles |
| title | Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers |
| title_full | Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers |
| title_fullStr | Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers |
| title_full_unstemmed | Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers |
| title_short | Developing a novel therapeutic and bioactive resin-based root canal sealer incorporated with silver polydopamine-modified hydroxyapatite fillers |
| title_sort | developing a novel therapeutic and bioactive resin based root canal sealer incorporated with silver polydopamine modified hydroxyapatite fillers |
| topic | Antibacterial Hydroxyapatite Polydopamine Remineralization Root canal sealer Silver nanoparticles |
| url | https://doi.org/10.1186/s12903-025-06469-2 |
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