Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan.
Poliovirus (PV) environmental surveillance (ES) plays an important role in the global eradication program and is crucial for monitoring silent PV circulation especially as clinical cases decrease. This study compared ES results using the novel bag-mediated filtration system (BMFS) with the current t...
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200551&type=printable |
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| author | Nicolette Angela Zhou Christine Susan Fagnant-Sperati Jeffry Hiroshi Shirai Salmaan Sharif Sohail Zahoor Zaidi Lubna Rehman Jaffer Hussain Rahim Agha Shahzad Shaukat Masroor Alam Adnan Khurshid Ghulam Mujtaba Muhammed Salman Rana Muhammed Safdar Abdirahman Mahamud Jamal Ahmed Sadaf Khan Alexandra Lynn Kossik Nicola Koren Beck Graciela Matrajt Humayun Asghar Ananda Sankar Bandyopadhyay David Scott Boyle John Scott Meschke |
| author_facet | Nicolette Angela Zhou Christine Susan Fagnant-Sperati Jeffry Hiroshi Shirai Salmaan Sharif Sohail Zahoor Zaidi Lubna Rehman Jaffer Hussain Rahim Agha Shahzad Shaukat Masroor Alam Adnan Khurshid Ghulam Mujtaba Muhammed Salman Rana Muhammed Safdar Abdirahman Mahamud Jamal Ahmed Sadaf Khan Alexandra Lynn Kossik Nicola Koren Beck Graciela Matrajt Humayun Asghar Ananda Sankar Bandyopadhyay David Scott Boyle John Scott Meschke |
| author_sort | Nicolette Angela Zhou |
| collection | DOAJ |
| description | Poliovirus (PV) environmental surveillance (ES) plays an important role in the global eradication program and is crucial for monitoring silent PV circulation especially as clinical cases decrease. This study compared ES results using the novel bag-mediated filtration system (BMFS) with the current two-phase separation method. From February to November 2016, BMFS and two-phase samples were collected concurrently from twelve sites in Pakistan (n = 117). Detection was higher in BMFS than two-phase samples for each Sabin-like (SL) PV serotype (p<0.001) and wild PV type 1 (WPV1) (p = 0.065). Seventeen sampling events were positive for WPV1, with eight discordant in favor of BMFS and two in favor of two-phase. A vaccine-derived PV type 2 was detected in one BMFS sample but not the matched two-phase. After the removal of SL PV type 2 (SL2) from the oral polio vaccine in April 2016, BMFS samples detected SL2 more frequently than two-phase (p = 0.016), with the last detection by either method occurring June 12, 2016. More frequent PV detection in BMFS compared to two-phase samples is likely due to the greater effective volume assayed (1620 mL vs. 150 mL). This study demonstrated that the BMFS achieves enhanced ES for all PV serotypes in an endemic country. |
| format | Article |
| id | doaj-art-e879fd10cf9f486a8c8fbfa80db0740f |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e879fd10cf9f486a8c8fbfa80db0740f2025-08-20T02:54:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020055110.1371/journal.pone.0200551Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan.Nicolette Angela ZhouChristine Susan Fagnant-SperatiJeffry Hiroshi ShiraiSalmaan SharifSohail Zahoor ZaidiLubna RehmanJaffer HussainRahim AghaShahzad ShaukatMasroor AlamAdnan KhurshidGhulam MujtabaMuhammed SalmanRana Muhammed SafdarAbdirahman MahamudJamal AhmedSadaf KhanAlexandra Lynn KossikNicola Koren BeckGraciela MatrajtHumayun AsgharAnanda Sankar BandyopadhyayDavid Scott BoyleJohn Scott MeschkePoliovirus (PV) environmental surveillance (ES) plays an important role in the global eradication program and is crucial for monitoring silent PV circulation especially as clinical cases decrease. This study compared ES results using the novel bag-mediated filtration system (BMFS) with the current two-phase separation method. From February to November 2016, BMFS and two-phase samples were collected concurrently from twelve sites in Pakistan (n = 117). Detection was higher in BMFS than two-phase samples for each Sabin-like (SL) PV serotype (p<0.001) and wild PV type 1 (WPV1) (p = 0.065). Seventeen sampling events were positive for WPV1, with eight discordant in favor of BMFS and two in favor of two-phase. A vaccine-derived PV type 2 was detected in one BMFS sample but not the matched two-phase. After the removal of SL PV type 2 (SL2) from the oral polio vaccine in April 2016, BMFS samples detected SL2 more frequently than two-phase (p = 0.016), with the last detection by either method occurring June 12, 2016. More frequent PV detection in BMFS compared to two-phase samples is likely due to the greater effective volume assayed (1620 mL vs. 150 mL). This study demonstrated that the BMFS achieves enhanced ES for all PV serotypes in an endemic country.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200551&type=printable |
| spellingShingle | Nicolette Angela Zhou Christine Susan Fagnant-Sperati Jeffry Hiroshi Shirai Salmaan Sharif Sohail Zahoor Zaidi Lubna Rehman Jaffer Hussain Rahim Agha Shahzad Shaukat Masroor Alam Adnan Khurshid Ghulam Mujtaba Muhammed Salman Rana Muhammed Safdar Abdirahman Mahamud Jamal Ahmed Sadaf Khan Alexandra Lynn Kossik Nicola Koren Beck Graciela Matrajt Humayun Asghar Ananda Sankar Bandyopadhyay David Scott Boyle John Scott Meschke Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. PLoS ONE |
| title | Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. |
| title_full | Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. |
| title_fullStr | Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. |
| title_full_unstemmed | Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. |
| title_short | Evaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan. |
| title_sort | evaluation of the bag mediated filtration system as a novel tool for poliovirus environmental surveillance results from a comparative field study in pakistan |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200551&type=printable |
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