Identification of Diverse Bacteriophages Associated with Bees and Hoverflies

Bacteriophages are the most numerous, ubiquitous, and diverse biological entities on the planet. Prior studies have identified bacteriophages associated with pathogenic and commensal microbiota of honeybees. In this study we expand on what is known about bacteriophages from the lineages <i>Cau...

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Main Authors: Rohan A. Bandoo, Simona Kraberger, Cahit Ozturk, Michael C. Lund, Qiyun Zhu, Chelsea Cook, Brian Smith, Arvind Varsani
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Viruses
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Online Access:https://www.mdpi.com/1999-4915/17/2/201
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author Rohan A. Bandoo
Simona Kraberger
Cahit Ozturk
Michael C. Lund
Qiyun Zhu
Chelsea Cook
Brian Smith
Arvind Varsani
author_facet Rohan A. Bandoo
Simona Kraberger
Cahit Ozturk
Michael C. Lund
Qiyun Zhu
Chelsea Cook
Brian Smith
Arvind Varsani
author_sort Rohan A. Bandoo
collection DOAJ
description Bacteriophages are the most numerous, ubiquitous, and diverse biological entities on the planet. Prior studies have identified bacteriophages associated with pathogenic and commensal microbiota of honeybees. In this study we expand on what is known about bacteriophages from the lineages <i>Caudoviricetes</i>, <i>Inoviridae</i>, and <i>Microviridae</i>, which are associated with honeybees (Apidae, <i>Apis mellifera</i>), solitary bees of the genus <i>Nomia</i> (Halictidae, <i>Nomia</i>), and hoverflies (Syrphidae). The complete genomes of seven caudoviruses, seven inoviruses, and 288 microviruses were assembled from honeybees (<i>n</i> = 286) and hoverflies in Arizona (<i>n</i> = 2). We used bacterial host predictive software and sequence read mapping programs to infer the commensal and transient bacterial hosts of pollinating insects. Lastly, this study explores the phylogenetic relationships of microviruses sampled from bees, opportunistically sampled pollinating insects such as hoverflies, and blackflies.
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spelling doaj-art-e86e60afc4d74f55bcf355d5bef5f42f2025-08-20T03:11:19ZengMDPI AGViruses1999-49152025-01-0117220110.3390/v17020201Identification of Diverse Bacteriophages Associated with Bees and HoverfliesRohan A. Bandoo0Simona Kraberger1Cahit Ozturk2Michael C. Lund3Qiyun Zhu4Chelsea Cook5Brian Smith6Arvind Varsani7School of Life Sciences, Arizona State University, Tempe, AZ 85287, USAThe Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ 85287, USASchool of Life Sciences, Arizona State University, Tempe, AZ 85287, USAThe Biodesign Center for Fundamental and Applied Microbiomics, Arizona State University, Tempe, AZ 85287, USASchool of Life Sciences, Arizona State University, Tempe, AZ 85287, USADepartment of Biological Sciences, Marquette University, Milwaukee, WI 53233, USASchool of Life Sciences, Arizona State University, Tempe, AZ 85287, USASchool of Life Sciences, Arizona State University, Tempe, AZ 85287, USABacteriophages are the most numerous, ubiquitous, and diverse biological entities on the planet. Prior studies have identified bacteriophages associated with pathogenic and commensal microbiota of honeybees. In this study we expand on what is known about bacteriophages from the lineages <i>Caudoviricetes</i>, <i>Inoviridae</i>, and <i>Microviridae</i>, which are associated with honeybees (Apidae, <i>Apis mellifera</i>), solitary bees of the genus <i>Nomia</i> (Halictidae, <i>Nomia</i>), and hoverflies (Syrphidae). The complete genomes of seven caudoviruses, seven inoviruses, and 288 microviruses were assembled from honeybees (<i>n</i> = 286) and hoverflies in Arizona (<i>n</i> = 2). We used bacterial host predictive software and sequence read mapping programs to infer the commensal and transient bacterial hosts of pollinating insects. Lastly, this study explores the phylogenetic relationships of microviruses sampled from bees, opportunistically sampled pollinating insects such as hoverflies, and blackflies.https://www.mdpi.com/1999-4915/17/2/201<i>Microviridae</i><i>Inoviridae</i><i>Caudovirictes</i>honeybeeshoverfliesNomia solitary bees
spellingShingle Rohan A. Bandoo
Simona Kraberger
Cahit Ozturk
Michael C. Lund
Qiyun Zhu
Chelsea Cook
Brian Smith
Arvind Varsani
Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
Viruses
<i>Microviridae</i>
<i>Inoviridae</i>
<i>Caudovirictes</i>
honeybees
hoverflies
Nomia solitary bees
title Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
title_full Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
title_fullStr Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
title_full_unstemmed Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
title_short Identification of Diverse Bacteriophages Associated with Bees and Hoverflies
title_sort identification of diverse bacteriophages associated with bees and hoverflies
topic <i>Microviridae</i>
<i>Inoviridae</i>
<i>Caudovirictes</i>
honeybees
hoverflies
Nomia solitary bees
url https://www.mdpi.com/1999-4915/17/2/201
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