Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities
Abstract This study investigates the in silico anti-arboviral potential of zoochemicals derived from the methanolic extract of Charybdis natator shell, alongside their larvicidal efficacy against Aedes aegypti 4th instar larvae. Through GC–MS analysis, 27 zoochemicals were identified, demonstrating...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-04-01
|
| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-025-00868-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849733393586061312 |
|---|---|
| author | Karnan Ramachandran Senthil Bakthavatchalam Shunmuga Vadivu Ramalingam Ramachandran Vinayagam Mukeshwaran Ramesh Sukumaran Marimuthu Zhi-Hong Wen Chandramohan Govindasamy Khalid M. Almutairi Yi-Hao Lo |
| author_facet | Karnan Ramachandran Senthil Bakthavatchalam Shunmuga Vadivu Ramalingam Ramachandran Vinayagam Mukeshwaran Ramesh Sukumaran Marimuthu Zhi-Hong Wen Chandramohan Govindasamy Khalid M. Almutairi Yi-Hao Lo |
| author_sort | Karnan Ramachandran |
| collection | DOAJ |
| description | Abstract This study investigates the in silico anti-arboviral potential of zoochemicals derived from the methanolic extract of Charybdis natator shell, alongside their larvicidal efficacy against Aedes aegypti 4th instar larvae. Through GC–MS analysis, 27 zoochemicals were identified, demonstrating promising in silico activity against molecular antiviral targets: DENV2 protease (PDB: 6MO1) for anti-dengue, RNA polymerase (PDB: 5U04) for anti-Zika, and nsP2 protease (PDB: 3TRK) for anti-chikungunya. A strong positive correlation (r = 0.726–0.889) in binding affinities (kcal/mol) suggests a consistent inhibitory mechanism across these targets. Furthermore, PASS analysis indicates higher probabilities of activity (Pa) for insecticidal properties compared to antiviral efficacy, highlighting their dual potential as larvicidal agents and antiviral candidates. The methanolic extract of Charybdis natator shell exhibited potent larvicidal activity against Aedes aegypti (LC₅₀ = 81.001 µg/mL) in a dose-dependent manner (R2 = 0.968). In silico analysis further elucidated its inhibitory action on key growth regulators of A. aegypti, underscoring its potential to disrupt larval development. These findings highlight the dual utility of C. natator shell extract in vector management and in mitigating the transmission of arboviral diseases such as Dengue, Zika, and Chikungunya. The extract's promise as an eco-friendly, cost-effective source for developing novel insecticidal and antiviral agents merits further exploration. Graphical Abstract |
| format | Article |
| id | doaj-art-e86a9e2dd4a548fdb875b1e63c85a64b |
| institution | DOAJ |
| issn | 2197-4365 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj-art-e86a9e2dd4a548fdb875b1e63c85a64b2025-08-20T03:08:02ZengSpringerOpenBioresources and Bioprocessing2197-43652025-04-0112112910.1186/s40643-025-00868-7Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activitiesKarnan Ramachandran0Senthil Bakthavatchalam1Shunmuga Vadivu Ramalingam2Ramachandran Vinayagam3Mukeshwaran Ramesh4Sukumaran Marimuthu5Zhi-Hong Wen6Chandramohan Govindasamy7Khalid M. Almutairi8Yi-Hao Lo9PG and Research Department of Zoology, Rajah Serfoji Government College (Autonomous), Affiliated to Bharathidasan UniversityDepartment of Chemistry, Faculty of Engineering and Technology, SRM Institute of Science and TechnologyDepartment of Biochemistry, SRM Dental College, Bharathi SalaiDepartment of Biotechnology, College of Life and Applied Sciences, Yeungnam UniversityACS Medical CollegePG and Research Department of Zoology, Rajah Serfoji Government College (Autonomous), Affiliated to Bharathidasan UniversityNational Museum of Marine Biology and AquariumDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud UniversityDepartment of Community Health Sciences, College of Applied Medical Sciences, King Saud UniversityDepartment of Family Medicine, Zuoying Armed Forces General HospitalAbstract This study investigates the in silico anti-arboviral potential of zoochemicals derived from the methanolic extract of Charybdis natator shell, alongside their larvicidal efficacy against Aedes aegypti 4th instar larvae. Through GC–MS analysis, 27 zoochemicals were identified, demonstrating promising in silico activity against molecular antiviral targets: DENV2 protease (PDB: 6MO1) for anti-dengue, RNA polymerase (PDB: 5U04) for anti-Zika, and nsP2 protease (PDB: 3TRK) for anti-chikungunya. A strong positive correlation (r = 0.726–0.889) in binding affinities (kcal/mol) suggests a consistent inhibitory mechanism across these targets. Furthermore, PASS analysis indicates higher probabilities of activity (Pa) for insecticidal properties compared to antiviral efficacy, highlighting their dual potential as larvicidal agents and antiviral candidates. The methanolic extract of Charybdis natator shell exhibited potent larvicidal activity against Aedes aegypti (LC₅₀ = 81.001 µg/mL) in a dose-dependent manner (R2 = 0.968). In silico analysis further elucidated its inhibitory action on key growth regulators of A. aegypti, underscoring its potential to disrupt larval development. These findings highlight the dual utility of C. natator shell extract in vector management and in mitigating the transmission of arboviral diseases such as Dengue, Zika, and Chikungunya. The extract's promise as an eco-friendly, cost-effective source for developing novel insecticidal and antiviral agents merits further exploration. Graphical Abstracthttps://doi.org/10.1186/s40643-025-00868-7AntiviralArbovirus vectorCharybdis natatorZoo-virucideCrab zoochemicals |
| spellingShingle | Karnan Ramachandran Senthil Bakthavatchalam Shunmuga Vadivu Ramalingam Ramachandran Vinayagam Mukeshwaran Ramesh Sukumaran Marimuthu Zhi-Hong Wen Chandramohan Govindasamy Khalid M. Almutairi Yi-Hao Lo Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities Bioresources and Bioprocessing Antiviral Arbovirus vector Charybdis natator Zoo-virucide Crab zoochemicals |
| title | Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities |
| title_full | Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities |
| title_fullStr | Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities |
| title_full_unstemmed | Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities |
| title_short | Unlocking the dual power of Charybdis natator shell: antiviral and larvicidal activities |
| title_sort | unlocking the dual power of charybdis natator shell antiviral and larvicidal activities |
| topic | Antiviral Arbovirus vector Charybdis natator Zoo-virucide Crab zoochemicals |
| url | https://doi.org/10.1186/s40643-025-00868-7 |
| work_keys_str_mv | AT karnanramachandran unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT senthilbakthavatchalam unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT shunmugavadivuramalingam unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT ramachandranvinayagam unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT mukeshwaranramesh unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT sukumaranmarimuthu unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT zhihongwen unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT chandramohangovindasamy unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT khalidmalmutairi unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities AT yihaolo unlockingthedualpowerofcharybdisnatatorshellantiviralandlarvicidalactivities |