Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts
<i>Cannabis sativa</i> L. has been traditionally used for its therapeutic properties, particularly in treating various skin conditions. This study explores the in vitro anti-aging potential of five distinct parts of <i>C. sativa</i> L. (inflorescence, seed, leaf, stem, and ro...
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2025-06-01
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| author | Pannita Kongtananeti Desy Liana Hla Myo Anuchit Phanumartwiwath Chitlada Areesantichai |
| author_facet | Pannita Kongtananeti Desy Liana Hla Myo Anuchit Phanumartwiwath Chitlada Areesantichai |
| author_sort | Pannita Kongtananeti |
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| description | <i>Cannabis sativa</i> L. has been traditionally used for its therapeutic properties, particularly in treating various skin conditions. This study explores the in vitro anti-aging potential of five distinct parts of <i>C. sativa</i> L. (inflorescence, seed, leaf, stem, and root) by analyzing their bioactive compounds and biological activities. Ultrasound-assisted extraction was employed using ethyl acetate as an extracting solvent, followed by chemical characterization via gas chromatography-mass spectrometry (GC-MS/MS) and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) analyses. The biological assessment included antioxidant, anti-inflammatory, anti-tyrosinase activities, and cytotoxicity evaluations. The inflorescence extract demonstrated the antioxidant activity, with a half-maximal inhibitory concentration (IC<sub>50</sub>) value of 3,849.01 ± 5.25 µg/mL against DPPH radicals and 31.19 ± 0.96% inhibition of NO radicals at 1.25 mg/mL. Notably, the stem extract exhibited the highest anti-tyrosinase activity, with an IC<sub>50</sub> value of 0.01 ± 0.00 mg/mL, and significantly inhibited 5-lipoxygenase (5-LOX) activity with an IC<sub>50</sub> value of <0.024 µg/mL. All extracts showed no cytotoxicity on HaCaT cells at a concentration of 10 µg/mL, indicating their potential safety for dermatological applications. The stem extract was abundant in phytosterols, triterpenoids, diterpenoids, unsaturated fatty acids, and phenolic compounds, which likely contribute to its anti-inflammatory and anti-tyrosinase effects. These findings suggest that the stem, traditionally considered as waste, could be a valuable raw material for developing dermatological treatments with strong anti-inflammatory and skin-brightening effects. |
| format | Article |
| id | doaj-art-e85a294dd5de457a8a1cdaffdee871ad |
| institution | Kabale University |
| issn | 2413-4155 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sci |
| spelling | doaj-art-e85a294dd5de457a8a1cdaffdee871ad2025-08-20T03:27:25ZengMDPI AGSci2413-41552025-06-01727710.3390/sci7020077Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems ByproductsPannita Kongtananeti0Desy Liana1Hla Myo2Anuchit Phanumartwiwath3Chitlada Areesantichai4College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandCollege of Public Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandCollege of Public Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandCollege of Public Health Sciences, Chulalongkorn University, Bangkok 10330, ThailandCollege of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand<i>Cannabis sativa</i> L. has been traditionally used for its therapeutic properties, particularly in treating various skin conditions. This study explores the in vitro anti-aging potential of five distinct parts of <i>C. sativa</i> L. (inflorescence, seed, leaf, stem, and root) by analyzing their bioactive compounds and biological activities. Ultrasound-assisted extraction was employed using ethyl acetate as an extracting solvent, followed by chemical characterization via gas chromatography-mass spectrometry (GC-MS/MS) and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) analyses. The biological assessment included antioxidant, anti-inflammatory, anti-tyrosinase activities, and cytotoxicity evaluations. The inflorescence extract demonstrated the antioxidant activity, with a half-maximal inhibitory concentration (IC<sub>50</sub>) value of 3,849.01 ± 5.25 µg/mL against DPPH radicals and 31.19 ± 0.96% inhibition of NO radicals at 1.25 mg/mL. Notably, the stem extract exhibited the highest anti-tyrosinase activity, with an IC<sub>50</sub> value of 0.01 ± 0.00 mg/mL, and significantly inhibited 5-lipoxygenase (5-LOX) activity with an IC<sub>50</sub> value of <0.024 µg/mL. All extracts showed no cytotoxicity on HaCaT cells at a concentration of 10 µg/mL, indicating their potential safety for dermatological applications. The stem extract was abundant in phytosterols, triterpenoids, diterpenoids, unsaturated fatty acids, and phenolic compounds, which likely contribute to its anti-inflammatory and anti-tyrosinase effects. These findings suggest that the stem, traditionally considered as waste, could be a valuable raw material for developing dermatological treatments with strong anti-inflammatory and skin-brightening effects.https://www.mdpi.com/2413-4155/7/2/77antioxidantanti-inflammatoryanti-tyrosinase<i>Cannabis sativa</i>mass spectroscopyultrasound-assisted extraction |
| spellingShingle | Pannita Kongtananeti Desy Liana Hla Myo Anuchit Phanumartwiwath Chitlada Areesantichai Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts Sci antioxidant anti-inflammatory anti-tyrosinase <i>Cannabis sativa</i> mass spectroscopy ultrasound-assisted extraction |
| title | Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts |
| title_full | Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts |
| title_fullStr | Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts |
| title_full_unstemmed | Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts |
| title_short | Exploring Anti-Inflammatory and Anti-Tyrosinase Potentials and Phytochemical Profiling of <i>Cannabis sativa</i> Stems Byproducts |
| title_sort | exploring anti inflammatory and anti tyrosinase potentials and phytochemical profiling of i cannabis sativa i stems byproducts |
| topic | antioxidant anti-inflammatory anti-tyrosinase <i>Cannabis sativa</i> mass spectroscopy ultrasound-assisted extraction |
| url | https://www.mdpi.com/2413-4155/7/2/77 |
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