Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models
Background. Quorum sensing (QS) systems play an important role in modulating biofilm formation. Recent studies have found that the QS molecules had complex effects on the host immune systems. In addition, regulatory T cells (Tregs), known as important negative regulators in the immune system, have b...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/4012912 |
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| author | Lei Feng Qingqing Xiang Qing Ai Zhengli Wang Yunhui Zhang Qi Lu |
| author_facet | Lei Feng Qingqing Xiang Qing Ai Zhengli Wang Yunhui Zhang Qi Lu |
| author_sort | Lei Feng |
| collection | DOAJ |
| description | Background. Quorum sensing (QS) systems play an important role in modulating biofilm formation. Recent studies have found that the QS molecules had complex effects on the host immune systems. In addition, regulatory T cells (Tregs), known as important negative regulators in the immune system, have been found upregulated in multiple chronic infections. Therefore, the QS systems were hypothesized to be involved in modulating Tregs in biofilm-associated infections. Object. To explore the effects of QS systems on Tregs in catheter-related Pseudomonas aeruginosa biofilm infection rat models. Method. Catheter-related Pseudomonas aeruginosa biofilm infection rat models were established; the bacterial clearance rates, total cell counts in bronchoalveolar lavage (BAL) fluid, pathological changes of lungs, and the levels of Foxp3, TGF-β1, and IL-10 in PAO1 strain group were examined and compared with the QS-mutant ΔlasRΔrhlR and ΔlasIΔrhlI groups. Results. In PAO1 group, the bacterial clearance rates were lower, total cell counts were higher, pathological changes were severer, and the levels of Foxp3, TGF-β1, and IL-10 were significantly higher compared with QS-mutant groups (p<0.05). No significant difference was observed between the two QS-mutant groups (p>0.05). Conclusion. QS systems can trigger host immune system, accompanied with the upregulation of Tregs. |
| format | Article |
| id | doaj-art-e8560f10e9524f9f8c78c542c6542043 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e8560f10e9524f9f8c78c542c65420432025-02-03T01:11:08ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/40129124012912Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat ModelsLei Feng0Qingqing Xiang1Qing Ai2Zhengli Wang3Yunhui Zhang4Qi Lu5Department of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaDepartment of Neonatology, Children’s Hospital of Chongqing Medical University, Chongqing 400014, ChinaBackground. Quorum sensing (QS) systems play an important role in modulating biofilm formation. Recent studies have found that the QS molecules had complex effects on the host immune systems. In addition, regulatory T cells (Tregs), known as important negative regulators in the immune system, have been found upregulated in multiple chronic infections. Therefore, the QS systems were hypothesized to be involved in modulating Tregs in biofilm-associated infections. Object. To explore the effects of QS systems on Tregs in catheter-related Pseudomonas aeruginosa biofilm infection rat models. Method. Catheter-related Pseudomonas aeruginosa biofilm infection rat models were established; the bacterial clearance rates, total cell counts in bronchoalveolar lavage (BAL) fluid, pathological changes of lungs, and the levels of Foxp3, TGF-β1, and IL-10 in PAO1 strain group were examined and compared with the QS-mutant ΔlasRΔrhlR and ΔlasIΔrhlI groups. Results. In PAO1 group, the bacterial clearance rates were lower, total cell counts were higher, pathological changes were severer, and the levels of Foxp3, TGF-β1, and IL-10 were significantly higher compared with QS-mutant groups (p<0.05). No significant difference was observed between the two QS-mutant groups (p>0.05). Conclusion. QS systems can trigger host immune system, accompanied with the upregulation of Tregs.http://dx.doi.org/10.1155/2016/4012912 |
| spellingShingle | Lei Feng Qingqing Xiang Qing Ai Zhengli Wang Yunhui Zhang Qi Lu Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models Mediators of Inflammation |
| title | Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models |
| title_full | Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models |
| title_fullStr | Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models |
| title_full_unstemmed | Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models |
| title_short | Effects of Quorum Sensing Systems on Regulatory T Cells in Catheter-Related Pseudomonas aeruginosa Biofilm Infection Rat Models |
| title_sort | effects of quorum sensing systems on regulatory t cells in catheter related pseudomonas aeruginosa biofilm infection rat models |
| url | http://dx.doi.org/10.1155/2016/4012912 |
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