Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment
To better understand the role of broiler and pig farms as reservoirs and disseminating vehicles of Extended-Spectrum β-lactamase (ESBL)-producing Escherichia coli (Ec), we performed a phylogenomic and plasmid analysis with a holistic view (human-animal-environmental interface). Here, we selected 15...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | One Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352771425000941 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850117709374685184 |
|---|---|
| author | Sandra Martínez-Álvarez Susana Sanz Pierre Châtre María de Toro Carmen Olarte Pauline François Isabel Benito Minerva Viguera Myriam Zarazaga Jean-Yves Madec Marisa Haenni Carmen Torres |
| author_facet | Sandra Martínez-Álvarez Susana Sanz Pierre Châtre María de Toro Carmen Olarte Pauline François Isabel Benito Minerva Viguera Myriam Zarazaga Jean-Yves Madec Marisa Haenni Carmen Torres |
| author_sort | Sandra Martínez-Álvarez |
| collection | DOAJ |
| description | To better understand the role of broiler and pig farms as reservoirs and disseminating vehicles of Extended-Spectrum β-lactamase (ESBL)-producing Escherichia coli (Ec), we performed a phylogenomic and plasmid analysis with a holistic view (human-animal-environmental interface). Here, we selected 15 ESBL-Ec recovered from the slurry, manure or air of pig (n = 7) and broiler farms (n = 8) in La Rioja (Spain) for Whole-Genome Sequencing (WGS). ESBL-E. coli isolates from a broiler farm (BF) all carried the blaSHV-12 gene associated with ST68/ST117/ST770/ST1684 and the new genetic lineage ST15985. On the contrary, all those from a pig farm (PF) carried the blaCTX-M-14 gene associated with ST453/ST542/ST4038, and 6/7 isolates co-harboured the colistin-resistance mcr-1.1 gene. The blaCTX-M-14 genes were located on IncI1 (90–110 kpb) or IncF plasmids (100–110 kpb), while the blaSHV-12 ones were located on IncHI2 (220–250 kbp) or on IncF plasmids (150–180 kpb). IncF-blaCTX-M-14 and IncHI2-blaSHV-12 plasmids carried additional genes mostly inserted in class 1 integrons (intI1-dfrA17-aadA5-qacE-sul1 or intI1-estX-psp-aadA2-cmlA1-aadA-qacL-IS440-sul3 respectively). The mcr-1.1 gene was located on an IncX4 plasmid (33 kpb). Pig and poultry production systems lead to the selection of different blaESBL genes, and both contributed to the global burden of antimicrobial resistance. |
| format | Article |
| id | doaj-art-e841bf7e920e4160b4e46dd412283d3e |
| institution | OA Journals |
| issn | 2352-7714 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | One Health |
| spelling | doaj-art-e841bf7e920e4160b4e46dd412283d3e2025-08-20T02:36:02ZengElsevierOne Health2352-77142025-06-012010105810.1016/j.onehlt.2025.101058Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environmentSandra Martínez-Álvarez0Susana Sanz1Pierre Châtre2María de Toro3Carmen Olarte4Pauline François5Isabel Benito6Minerva Viguera7Myriam Zarazaga8Jean-Yves Madec9Marisa Haenni10Carmen Torres11Department of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainANSES – Université de Lyon, Unité Antibiorésitance et Virulence Bactériennes, Lyon, FranceGenomics and Bioinformatics Core Facility, Center for Biomedical Research of La Rioja (CIBIR), Logroño, SpainDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainANSES – Université de Lyon, Unité Antibiorésitance et Virulence Bactériennes, Lyon, FranceDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, SpainANSES – Université de Lyon, Unité Antibiorésitance et Virulence Bactériennes, Lyon, FranceANSES – Université de Lyon, Unité Antibiorésitance et Virulence Bactériennes, Lyon, FranceDepartment of Food and Agriculture, Faculty of Science and Technology, University of La Rioja, 26006 Logroño, Spain; Corresponding author at: Area of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of La Rioja, Madre de Dios 53, 26006 Logroño, Spain.To better understand the role of broiler and pig farms as reservoirs and disseminating vehicles of Extended-Spectrum β-lactamase (ESBL)-producing Escherichia coli (Ec), we performed a phylogenomic and plasmid analysis with a holistic view (human-animal-environmental interface). Here, we selected 15 ESBL-Ec recovered from the slurry, manure or air of pig (n = 7) and broiler farms (n = 8) in La Rioja (Spain) for Whole-Genome Sequencing (WGS). ESBL-E. coli isolates from a broiler farm (BF) all carried the blaSHV-12 gene associated with ST68/ST117/ST770/ST1684 and the new genetic lineage ST15985. On the contrary, all those from a pig farm (PF) carried the blaCTX-M-14 gene associated with ST453/ST542/ST4038, and 6/7 isolates co-harboured the colistin-resistance mcr-1.1 gene. The blaCTX-M-14 genes were located on IncI1 (90–110 kpb) or IncF plasmids (100–110 kpb), while the blaSHV-12 ones were located on IncHI2 (220–250 kbp) or on IncF plasmids (150–180 kpb). IncF-blaCTX-M-14 and IncHI2-blaSHV-12 plasmids carried additional genes mostly inserted in class 1 integrons (intI1-dfrA17-aadA5-qacE-sul1 or intI1-estX-psp-aadA2-cmlA1-aadA-qacL-IS440-sul3 respectively). The mcr-1.1 gene was located on an IncX4 plasmid (33 kpb). Pig and poultry production systems lead to the selection of different blaESBL genes, and both contributed to the global burden of antimicrobial resistance.http://www.sciencedirect.com/science/article/pii/S2352771425000941AMRPoultryPigOne HealthGeneticsIntegrons |
| spellingShingle | Sandra Martínez-Álvarez Susana Sanz Pierre Châtre María de Toro Carmen Olarte Pauline François Isabel Benito Minerva Viguera Myriam Zarazaga Jean-Yves Madec Marisa Haenni Carmen Torres Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment One Health AMR Poultry Pig One Health Genetics Integrons |
| title | Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment |
| title_full | Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment |
| title_fullStr | Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment |
| title_full_unstemmed | Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment |
| title_short | Interplay between bacterial clones and plasmids in the spread of blaSHV-12- and blaCTX-M-14-carrying Escherichia coli from the livestock environment |
| title_sort | interplay between bacterial clones and plasmids in the spread of blashv 12 and blactx m 14 carrying escherichia coli from the livestock environment |
| topic | AMR Poultry Pig One Health Genetics Integrons |
| url | http://www.sciencedirect.com/science/article/pii/S2352771425000941 |
| work_keys_str_mv | AT sandramartinezalvarez interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT susanasanz interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT pierrechatre interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT mariadetoro interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT carmenolarte interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT paulinefrancois interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT isabelbenito interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT minervaviguera interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT myriamzarazaga interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT jeanyvesmadec interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT marisahaenni interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment AT carmentorres interplaybetweenbacterialclonesandplasmidsinthespreadofblashv12andblactxm14carryingescherichiacolifromthelivestockenvironment |