Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis
The aim of this study was to evaluate the most frequent lesions in the bone marrow of dogs naturally infected by Leishmania (Leishmania) chagasi. Thirty-three dogs sacrificed at the Zoonosis Control Center of Araçatuba, a municipality endemic for visceral leishmaniasis (VL), were used. The animals...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2014/150582 |
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| author | Claudia Momo Ana Paula Prudente Jacintho Pamela Rodrigues Reina Moreira Danísio Prado Munari Gisele Fabrino Machado Rosemeri de Oliveira Vasconcelos |
| author_facet | Claudia Momo Ana Paula Prudente Jacintho Pamela Rodrigues Reina Moreira Danísio Prado Munari Gisele Fabrino Machado Rosemeri de Oliveira Vasconcelos |
| author_sort | Claudia Momo |
| collection | DOAJ |
| description | The aim of this study was to evaluate the most frequent lesions in the bone marrow of dogs naturally infected by Leishmania (Leishmania) chagasi. Thirty-three dogs sacrificed at the Zoonosis Control Center of Araçatuba, a municipality endemic for visceral leishmaniasis (VL), were used. The animals were classified as asymptomatic, oligosymptomatic, and symptomatic groups. At the necropsy, bone marrow samples were collected from the femur, fixed, processed, and stained with hematoxylin and eosin. The lesion intensity was classified as mild, moderate, or severe. The parasite load was determined using immunohistochemistry. The most important lesions consisted of multifocal to diffuse granulomas, megakaryocytic dysplasia, and medullary aplasia. There were no statistical differences between the three clinical groups regarding parasite load and lesion intensity. Asymptomatic dogs also presented high parasitism in the bone marrow as dogs with clinical signs of VL. It was concluded that, regardless of clinical group, the bone marrow is a site for multiplication of Leishmania chagasi. Possibly, the bone marrow dysplasia may arise from the presence of many parasitized and activated macrophages in this organ. Consequently, it affects the profile of hematopoietic cells in the bone marrow and systemic circulation. |
| format | Article |
| id | doaj-art-e83cb9d2749144cd9851739a80e09586 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-e83cb9d2749144cd9851739a80e095862025-02-03T06:14:13ZengWileyVeterinary Medicine International2090-81132042-00482014-01-01201410.1155/2014/150582150582Morphological Changes in the Bone Marrow of the Dogs with Visceral LeishmaniasisClaudia Momo0Ana Paula Prudente Jacintho1Pamela Rodrigues Reina Moreira2Danísio Prado Munari3Gisele Fabrino Machado4Rosemeri de Oliveira Vasconcelos5The Postgraduate Program on Veterinary Medicine, FCAV-UNESP, Jaboticabal, SP, BrazilThe Postgraduate Program on Veterinary Medicine, FCAV-UNESP, Jaboticabal, SP, BrazilThe Postgraduate Program on Veterinary Medicine, FCAV-UNESP, Jaboticabal, SP, BrazilDepartamento de Ciências Exatas, FCAV-UNESP, Jaboticabal, SP, BrazilDepartamento de Clínica, Cirurgia e Reprodução Animal, FMVA-UNESP, Araçatuba, SP, BrazilDepartamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias (FCAV-UNESP), Via de Acesso Professor Paulo Donato Castellane, s/n°, Bairro Industrial, 14.884-900 Jaboticabal, SP, BrazilThe aim of this study was to evaluate the most frequent lesions in the bone marrow of dogs naturally infected by Leishmania (Leishmania) chagasi. Thirty-three dogs sacrificed at the Zoonosis Control Center of Araçatuba, a municipality endemic for visceral leishmaniasis (VL), were used. The animals were classified as asymptomatic, oligosymptomatic, and symptomatic groups. At the necropsy, bone marrow samples were collected from the femur, fixed, processed, and stained with hematoxylin and eosin. The lesion intensity was classified as mild, moderate, or severe. The parasite load was determined using immunohistochemistry. The most important lesions consisted of multifocal to diffuse granulomas, megakaryocytic dysplasia, and medullary aplasia. There were no statistical differences between the three clinical groups regarding parasite load and lesion intensity. Asymptomatic dogs also presented high parasitism in the bone marrow as dogs with clinical signs of VL. It was concluded that, regardless of clinical group, the bone marrow is a site for multiplication of Leishmania chagasi. Possibly, the bone marrow dysplasia may arise from the presence of many parasitized and activated macrophages in this organ. Consequently, it affects the profile of hematopoietic cells in the bone marrow and systemic circulation.http://dx.doi.org/10.1155/2014/150582 |
| spellingShingle | Claudia Momo Ana Paula Prudente Jacintho Pamela Rodrigues Reina Moreira Danísio Prado Munari Gisele Fabrino Machado Rosemeri de Oliveira Vasconcelos Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis Veterinary Medicine International |
| title | Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis |
| title_full | Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis |
| title_fullStr | Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis |
| title_full_unstemmed | Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis |
| title_short | Morphological Changes in the Bone Marrow of the Dogs with Visceral Leishmaniasis |
| title_sort | morphological changes in the bone marrow of the dogs with visceral leishmaniasis |
| url | http://dx.doi.org/10.1155/2014/150582 |
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