The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models
With increasing evidence showing the connections between the microbiome, neurophysiology, and behavior, our research endeavors to investigate whether the consumption of a combination of two postbiotics with antioxidant effects can affect behavior regulation in model species. Here, we worked with a c...
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2024-12-01
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| author | Vanesa Robles Ferran Balaguer Miren Maicas Juan Manuel Martínez-Vázquez Patricia Martorell Marta Tortajada Daniel Ramón David G. Valcarce |
| author_facet | Vanesa Robles Ferran Balaguer Miren Maicas Juan Manuel Martínez-Vázquez Patricia Martorell Marta Tortajada Daniel Ramón David G. Valcarce |
| author_sort | Vanesa Robles |
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| description | With increasing evidence showing the connections between the microbiome, neurophysiology, and behavior, our research endeavors to investigate whether the consumption of a combination of two postbiotics with antioxidant effects can affect behavior regulation in model species. Here, we worked with a combination (1:1 ratio) of heat-treated <i>Bifidobacterium longum</i> subsp. <i>longum</i> ES1 (CECT7347) and <i>Lacticaseibacillus rhamnosus</i> BPL15 (CECT8361) as a dietary supplement. To examine the potential benefit of using this formulation to alleviate anxiety-like behavior, we employed two model species, <i>Caenorhabditis elegans</i> and adult <i>Danio rerio</i>. In <i>C. elegans</i>, the postbiotic supplementation reduced the anxiety-related behavior analyzed by means of the octanol avoidance test. In zebrafish, the novel tank test indicated a different swimming pattern 2 and 4 months after the animals were fed with the postbiotic combination. While fish did not exhibit any variance in their locomotion parameters such as pace and speed, they showed a statistically significant preference to spend more time in the upper zone of the water tank, a behavior that is correlated with a lower anxiety-like behavior in these species. Our aim with this study is to present evidence that can be used to develop whole-cell postbiotic-based novel and innovative dietary supplements for anxiety-related conditions. |
| format | Article |
| id | doaj-art-e83854e35913451d957dacfdfb85a36c |
| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Cells |
| spelling | doaj-art-e83854e35913451d957dacfdfb85a36c2025-08-20T01:55:31ZengMDPI AGCells2073-44092024-12-011323200610.3390/cells13232006The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal ModelsVanesa Robles0Ferran Balaguer1Miren Maicas2Juan Manuel Martínez-Vázquez3Patricia Martorell4Marta Tortajada5Daniel Ramón6David G. Valcarce7Cell Biology Area, Molecular Biology Department, Campus de Vegazana s/n, Universidad de León, 24071 León, SpainArcher Daniels Midland, Nutrition, Health & Wellness, Biopolis S.L. Parc Científic Universitat de València, C/Catedrático Agustín Escardino Benlloch, 9, 46980 Paterna, SpainArcher Daniels Midland, Nutrition, Health & Wellness, Biopolis S.L. Parc Científic Universitat de València, C/Catedrático Agustín Escardino Benlloch, 9, 46980 Paterna, SpainInstituto Español de Oceanografía, Centro Oceanográfico de Santander (COST-IEO), CSIC, Calle Severiano Ballesteros 16, 39004 Santander, SpainArcher Daniels Midland, Nutrition, Health & Wellness, Biopolis S.L. Parc Científic Universitat de València, C/Catedrático Agustín Escardino Benlloch, 9, 46980 Paterna, SpainArcher Daniels Midland, Nutrition, Health & Wellness, Biopolis S.L. Parc Científic Universitat de València, C/Catedrático Agustín Escardino Benlloch, 9, 46980 Paterna, SpainArcher Daniels Midland, Nutrition, Health & Wellness, Biopolis S.L. Parc Científic Universitat de València, C/Catedrático Agustín Escardino Benlloch, 9, 46980 Paterna, SpainCell Biology Area, Molecular Biology Department, Campus de Vegazana s/n, Universidad de León, 24071 León, SpainWith increasing evidence showing the connections between the microbiome, neurophysiology, and behavior, our research endeavors to investigate whether the consumption of a combination of two postbiotics with antioxidant effects can affect behavior regulation in model species. Here, we worked with a combination (1:1 ratio) of heat-treated <i>Bifidobacterium longum</i> subsp. <i>longum</i> ES1 (CECT7347) and <i>Lacticaseibacillus rhamnosus</i> BPL15 (CECT8361) as a dietary supplement. To examine the potential benefit of using this formulation to alleviate anxiety-like behavior, we employed two model species, <i>Caenorhabditis elegans</i> and adult <i>Danio rerio</i>. In <i>C. elegans</i>, the postbiotic supplementation reduced the anxiety-related behavior analyzed by means of the octanol avoidance test. In zebrafish, the novel tank test indicated a different swimming pattern 2 and 4 months after the animals were fed with the postbiotic combination. While fish did not exhibit any variance in their locomotion parameters such as pace and speed, they showed a statistically significant preference to spend more time in the upper zone of the water tank, a behavior that is correlated with a lower anxiety-like behavior in these species. Our aim with this study is to present evidence that can be used to develop whole-cell postbiotic-based novel and innovative dietary supplements for anxiety-related conditions.https://www.mdpi.com/2073-4409/13/23/2006<i>Bifidobacterium</i><i>Lacticaseibacillus</i>anxiety-likepostbiotics |
| spellingShingle | Vanesa Robles Ferran Balaguer Miren Maicas Juan Manuel Martínez-Vázquez Patricia Martorell Marta Tortajada Daniel Ramón David G. Valcarce The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models Cells <i>Bifidobacterium</i> <i>Lacticaseibacillus</i> anxiety-like postbiotics |
| title | The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models |
| title_full | The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models |
| title_fullStr | The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models |
| title_full_unstemmed | The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models |
| title_short | The Effect of the Combination of Two Postbiotics on Anxiety-like Behavior in Animal Models |
| title_sort | effect of the combination of two postbiotics on anxiety like behavior in animal models |
| topic | <i>Bifidobacterium</i> <i>Lacticaseibacillus</i> anxiety-like postbiotics |
| url | https://www.mdpi.com/2073-4409/13/23/2006 |
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