In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles
The 5′ untranslated region (5′ UTR) of rodent hepacivirus (RHV) and pegivirus (RPgV) contains sequence homology to the HCV type III internal ribosome entry sites (IRES). Utilizing a monocistronic expression vector with an RNA polymerase I promoter to drive transcription, we show cell-specific IRES t...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2021/5569844 |
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| author | Stuart Sims Kevin Michaelsen Sara Burkhard Cornel Fraefel |
| author_facet | Stuart Sims Kevin Michaelsen Sara Burkhard Cornel Fraefel |
| author_sort | Stuart Sims |
| collection | DOAJ |
| description | The 5′ untranslated region (5′ UTR) of rodent hepacivirus (RHV) and pegivirus (RPgV) contains sequence homology to the HCV type III internal ribosome entry sites (IRES). Utilizing a monocistronic expression vector with an RNA polymerase I promoter to drive transcription, we show cell-specific IRES translation and regions within the IRES required for full functionality. Focusing on RHV, we further pseudotyped lentivirus with RHV and showed cell surface expression of the envelope proteins and transduction of murine hepatocytes and we then constructed full-length RHV and RPgV replicons with reporter genes. Using the replicon system, we show that the RHV NS3-4A protease cleaves a mitochondrial antiviral signaling protein reporter. However, liver-derived cells did not readily support the complete viral life cycle. |
| format | Article |
| id | doaj-art-e82ef88a16e94a648ce1d623424d76b0 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-e82ef88a16e94a648ce1d623424d76b02025-02-03T01:27:24ZengWileyAdvances in Virology1687-86391687-86472021-01-01202110.1155/2021/55698445569844In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of PseudoparticlesStuart Sims0Kevin Michaelsen1Sara Burkhard2Cornel Fraefel3Institute of Virology, University of Zurich, Zurich, SwitzerlandInstitute of Virology, University of Zurich, Zurich, SwitzerlandDepartment of Infectious Diseases, University Hospital of Zurich, Zurich, SwitzerlandInstitute of Virology, University of Zurich, Zurich, SwitzerlandThe 5′ untranslated region (5′ UTR) of rodent hepacivirus (RHV) and pegivirus (RPgV) contains sequence homology to the HCV type III internal ribosome entry sites (IRES). Utilizing a monocistronic expression vector with an RNA polymerase I promoter to drive transcription, we show cell-specific IRES translation and regions within the IRES required for full functionality. Focusing on RHV, we further pseudotyped lentivirus with RHV and showed cell surface expression of the envelope proteins and transduction of murine hepatocytes and we then constructed full-length RHV and RPgV replicons with reporter genes. Using the replicon system, we show that the RHV NS3-4A protease cleaves a mitochondrial antiviral signaling protein reporter. However, liver-derived cells did not readily support the complete viral life cycle.http://dx.doi.org/10.1155/2021/5569844 |
| spellingShingle | Stuart Sims Kevin Michaelsen Sara Burkhard Cornel Fraefel In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles Advances in Virology |
| title | In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles |
| title_full | In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles |
| title_fullStr | In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles |
| title_full_unstemmed | In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles |
| title_short | In Vitro Comparison of the Internal Ribosomal Entry Site Activity from Rodent Hepacivirus and Pegivirus and Construction of Pseudoparticles |
| title_sort | in vitro comparison of the internal ribosomal entry site activity from rodent hepacivirus and pegivirus and construction of pseudoparticles |
| url | http://dx.doi.org/10.1155/2021/5569844 |
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