Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers
The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis,...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/489060 |
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| _version_ | 1849691357539467264 |
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| author | Rubén Ferreira Anna Aviñó Ricardo Pérez-Tomás Raimundo Gargallo Ramon Eritja |
| author_facet | Rubén Ferreira Anna Aviñó Ricardo Pérez-Tomás Raimundo Gargallo Ramon Eritja |
| author_sort | Rubén Ferreira |
| collection | DOAJ |
| description | The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4–6. |
| format | Article |
| id | doaj-art-e829026e7e5846aead77a3e7cece4e9c |
| institution | DOAJ |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-e829026e7e5846aead77a3e7cece4e9c2025-08-20T03:21:03ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/489060489060Synthesis and G-Quadruplex-Binding Properties of Defined Acridine OligomersRubén Ferreira0Anna Aviñó1Ricardo Pérez-Tomás2Raimundo Gargallo3Ramon Eritja4Institute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 15, 08028 Barcelona, SpainInstitute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 15, 08028 Barcelona, SpainCancer Cell Biology Research Group, Department of Pathology and Experimental Therapeutics, Faculty of Medicine, University of Barcelona Campus Bellvitge, Feixa Llarga, L'Hospitalet de Llobregat, 08907 Barcelona, SpainDepartment of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028 Barcelona, SpainInstitute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 15, 08028 Barcelona, SpainThe synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4–6.http://dx.doi.org/10.4061/2010/489060 |
| spellingShingle | Rubén Ferreira Anna Aviñó Ricardo Pérez-Tomás Raimundo Gargallo Ramon Eritja Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers Journal of Nucleic Acids |
| title | Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers |
| title_full | Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers |
| title_fullStr | Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers |
| title_full_unstemmed | Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers |
| title_short | Synthesis and G-Quadruplex-Binding Properties of Defined Acridine Oligomers |
| title_sort | synthesis and g quadruplex binding properties of defined acridine oligomers |
| url | http://dx.doi.org/10.4061/2010/489060 |
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