Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application
Abstract Mustard, a major source of edible and industrial grade oils in the Indian subcontinent and various regions of Australia, Eastern Europe, and Canada, is also a protein resource for the animal feed industry. Silique and seed size are key traits for mustard improvement, but their inheritance m...
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Nature Portfolio
2025-07-01
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| author | Javed Akhatar Anna Goyal Meenakshi Mittal Heena Verma Kaur Gurpreet Beerpal Kaur Surinder S. Banga Chhaya Atri |
| author_facet | Javed Akhatar Anna Goyal Meenakshi Mittal Heena Verma Kaur Gurpreet Beerpal Kaur Surinder S. Banga Chhaya Atri |
| author_sort | Javed Akhatar |
| collection | DOAJ |
| description | Abstract Mustard, a major source of edible and industrial grade oils in the Indian subcontinent and various regions of Australia, Eastern Europe, and Canada, is also a protein resource for the animal feed industry. Silique and seed size are key traits for mustard improvement, but their inheritance mechanisms are not fully understood. We evaluated 92 inbred lines for silique length, seeds per silique, seed size, and rupture energy required to shatter a pod at three levels of nitrogen application in two crop seasons. Genotypes showed large phenotypic variations and a continuous distribution for all silique-related traits, suggesting a quantitative inheritance. Genotype × nitrogen interactions were significant for silique length, seeds per silique, and rupture energy. Association analysis identified 59 significant SNPs, whose annotations facilitated the prediction of 16 important genes underlying observed phenotypic variations. These genes are associated with silique formation (SHP2), grain formation (BG1, BG4), cell elongation (BRI1), grain filling (AT1G12500, AT1G77610, SWEET1, and AT3G14410), and silique shattering (UBP15, CO, INDEHISCENT, AGAMOUS1, FRUITFULL, and SHP2). RNA-seq data from 18 mustard genotypes revealed expression-level variations for identified candidate genes. Upregulation of SHP2 for SPS and resistance to silique shattering was observed, while downregulation of BG4 was observed in two genotypes with smallest seeds. This study provides valuable insight that may facilitate the marker-assisted selection (MAS) breeding for silique traits. |
| format | Article |
| id | doaj-art-e81222e3cc2641d3ac71adae1b51e4dc |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-e81222e3cc2641d3ac71adae1b51e4dc2025-08-20T03:03:37ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-07758-0Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen applicationJaved Akhatar0Anna Goyal1Meenakshi Mittal2Heena Verma3Kaur Gurpreet4Beerpal Kaur5Surinder S. Banga6Chhaya Atri7Department of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityDepartment of Plant Breeding and Genetics, Punjab Agricultural UniversityAbstract Mustard, a major source of edible and industrial grade oils in the Indian subcontinent and various regions of Australia, Eastern Europe, and Canada, is also a protein resource for the animal feed industry. Silique and seed size are key traits for mustard improvement, but their inheritance mechanisms are not fully understood. We evaluated 92 inbred lines for silique length, seeds per silique, seed size, and rupture energy required to shatter a pod at three levels of nitrogen application in two crop seasons. Genotypes showed large phenotypic variations and a continuous distribution for all silique-related traits, suggesting a quantitative inheritance. Genotype × nitrogen interactions were significant for silique length, seeds per silique, and rupture energy. Association analysis identified 59 significant SNPs, whose annotations facilitated the prediction of 16 important genes underlying observed phenotypic variations. These genes are associated with silique formation (SHP2), grain formation (BG1, BG4), cell elongation (BRI1), grain filling (AT1G12500, AT1G77610, SWEET1, and AT3G14410), and silique shattering (UBP15, CO, INDEHISCENT, AGAMOUS1, FRUITFULL, and SHP2). RNA-seq data from 18 mustard genotypes revealed expression-level variations for identified candidate genes. Upregulation of SHP2 for SPS and resistance to silique shattering was observed, while downregulation of BG4 was observed in two genotypes with smallest seeds. This study provides valuable insight that may facilitate the marker-assisted selection (MAS) breeding for silique traits.https://doi.org/10.1038/s41598-025-07758-0Rapeseed-mustardGenome-wide association studiesCandidate genesPod shatteringRNA-seqRupture energy |
| spellingShingle | Javed Akhatar Anna Goyal Meenakshi Mittal Heena Verma Kaur Gurpreet Beerpal Kaur Surinder S. Banga Chhaya Atri Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application Scientific Reports Rapeseed-mustard Genome-wide association studies Candidate genes Pod shattering RNA-seq Rupture energy |
| title | Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application |
| title_full | Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application |
| title_fullStr | Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application |
| title_full_unstemmed | Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application |
| title_short | Genetic analysis of silique and seed traits in Brassica juncea (L.) Czern. under differential doses of nitrogen application |
| title_sort | genetic analysis of silique and seed traits in brassica juncea l czern under differential doses of nitrogen application |
| topic | Rapeseed-mustard Genome-wide association studies Candidate genes Pod shattering RNA-seq Rupture energy |
| url | https://doi.org/10.1038/s41598-025-07758-0 |
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