Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation
<i>Calocybe indica</i> (milky mushroom), an edible mushroom with significant nutritional value, shows potential for cultivation in subtropical regions. Investigating the composition and diversity of the microbial community structure of the casing materials of <i>C. indica</i>...
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MDPI AG
2025-06-01
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| Series: | Horticulturae |
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| author | Jia-Ling Zhao Yuan-Yuan Shao Bin Liu |
| author_facet | Jia-Ling Zhao Yuan-Yuan Shao Bin Liu |
| author_sort | Jia-Ling Zhao |
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| description | <i>Calocybe indica</i> (milky mushroom), an edible mushroom with significant nutritional value, shows potential for cultivation in subtropical regions. Investigating the composition and diversity of the microbial community structure of the casing materials of <i>C. indica</i> is of great significance for understanding the stable yield of the mushroom. This study evaluated four casing materials—loamy soil (LS), loamy soil + cow dung (LS + CD), loamy soil + sand (LS + S), and plant ash (PA)—for their effects on mushroom yield, soil physicochemical properties, and microbial dynamics. The results demonstrated that LS + CD significantly enhanced the yield (2078.50 g) and fruiting body quality, with the shortest pinhead formation time (7.67 days) and superior morphological traits (e.g., cap diameter: 10.10 cm). Physicochemical analysis revealed LS + CD’s elevated moisture retention (19.7%), nutrient availability (e.g., available P: 59.63 mg/kg), and microbial biomass (C: 399.22 mg/kg), alongside a distinct microbial community dominated by Basidiomycota and Actinobacteria. Conversely, LS + S exhibited poor performance due to low water retention and nutrient deficiencies. Redundancy analysis highlighted strong correlations between soil nutrients (nitrogen, potassium, phosphorus) and microbial composition, with LS + CD fostering a microbiome conducive to mushroom growth. These findings underscore LS + CD as the optimal casing material for <i>C. indica</i> cultivation, improving both yield and soil health. Future studies should explore the functional roles of key microbes and refine organic amendments for sustainable practices. |
| format | Article |
| id | doaj-art-e81085e19f9041cd95b4ea546e545cfd |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-e81085e19f9041cd95b4ea546e545cfd2025-08-20T02:21:10ZengMDPI AGHorticulturae2311-75242025-06-0111666710.3390/horticulturae11060667Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) CultivationJia-Ling Zhao0Yuan-Yuan Shao1Bin Liu2Institute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning 530004, ChinaKey Laboratory of Environment Change and Resources Use in Beibu Gulf, Nanning Normal University, Nanning 530001, ChinaInstitute of Applied Microbiology, College of Agriculture, Guangxi University, Nanning 530004, China<i>Calocybe indica</i> (milky mushroom), an edible mushroom with significant nutritional value, shows potential for cultivation in subtropical regions. Investigating the composition and diversity of the microbial community structure of the casing materials of <i>C. indica</i> is of great significance for understanding the stable yield of the mushroom. This study evaluated four casing materials—loamy soil (LS), loamy soil + cow dung (LS + CD), loamy soil + sand (LS + S), and plant ash (PA)—for their effects on mushroom yield, soil physicochemical properties, and microbial dynamics. The results demonstrated that LS + CD significantly enhanced the yield (2078.50 g) and fruiting body quality, with the shortest pinhead formation time (7.67 days) and superior morphological traits (e.g., cap diameter: 10.10 cm). Physicochemical analysis revealed LS + CD’s elevated moisture retention (19.7%), nutrient availability (e.g., available P: 59.63 mg/kg), and microbial biomass (C: 399.22 mg/kg), alongside a distinct microbial community dominated by Basidiomycota and Actinobacteria. Conversely, LS + S exhibited poor performance due to low water retention and nutrient deficiencies. Redundancy analysis highlighted strong correlations between soil nutrients (nitrogen, potassium, phosphorus) and microbial composition, with LS + CD fostering a microbiome conducive to mushroom growth. These findings underscore LS + CD as the optimal casing material for <i>C. indica</i> cultivation, improving both yield and soil health. Future studies should explore the functional roles of key microbes and refine organic amendments for sustainable practices.https://www.mdpi.com/2311-7524/11/6/667<i>Calocybe indica</i>casing materialhigh throughputmicrobial diversity |
| spellingShingle | Jia-Ling Zhao Yuan-Yuan Shao Bin Liu Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation Horticulturae <i>Calocybe indica</i> casing material high throughput microbial diversity |
| title | Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation |
| title_full | Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation |
| title_fullStr | Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation |
| title_full_unstemmed | Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation |
| title_short | Microbiome Dynamics in Four Different Casing Materials Used for Milky Mushroom (<i>Calocybe indica</i>) Cultivation |
| title_sort | microbiome dynamics in four different casing materials used for milky mushroom i calocybe indica i cultivation |
| topic | <i>Calocybe indica</i> casing material high throughput microbial diversity |
| url | https://www.mdpi.com/2311-7524/11/6/667 |
| work_keys_str_mv | AT jialingzhao microbiomedynamicsinfourdifferentcasingmaterialsusedformilkymushroomicalocybeindicaicultivation AT yuanyuanshao microbiomedynamicsinfourdifferentcasingmaterialsusedformilkymushroomicalocybeindicaicultivation AT binliu microbiomedynamicsinfourdifferentcasingmaterialsusedformilkymushroomicalocybeindicaicultivation |