Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.

Ankylosing spondylitis (AS) is a common inflammatory arthritic condition. Overt inflammatory bowel disease (IBD) occurs in about 10% of AS patients, and in addition 70% of AS cases may have subclinical terminal ileitis. Spondyloarthritis is also common in IBD patients. We therefore tested Crohn'...

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Main Authors: Patrick Danoy, Karena Pryce, Johanna Hadler, Linda A Bradbury, Claire Farrar, Jennifer Pointon, Australo-Anglo-American Spondyloarthritis Consortium, Michael Ward, Michael Weisman, John D Reveille, B Paul Wordsworth, Millicent A Stone, Spondyloarthritis Research Consortium of Canada, Walter P Maksymowych, Proton Rahman, Dafna Gladman, Robert D Inman, Matthew A Brown
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-12-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1001195&type=printable
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author Patrick Danoy
Karena Pryce
Johanna Hadler
Linda A Bradbury
Claire Farrar
Jennifer Pointon
Australo-Anglo-American Spondyloarthritis Consortium
Michael Ward
Michael Weisman
John D Reveille
B Paul Wordsworth
Millicent A Stone
Spondyloarthritis Research Consortium of Canada
Walter P Maksymowych
Proton Rahman
Dafna Gladman
Robert D Inman
Matthew A Brown
author_facet Patrick Danoy
Karena Pryce
Johanna Hadler
Linda A Bradbury
Claire Farrar
Jennifer Pointon
Australo-Anglo-American Spondyloarthritis Consortium
Michael Ward
Michael Weisman
John D Reveille
B Paul Wordsworth
Millicent A Stone
Spondyloarthritis Research Consortium of Canada
Walter P Maksymowych
Proton Rahman
Dafna Gladman
Robert D Inman
Matthew A Brown
author_sort Patrick Danoy
collection DOAJ
description Ankylosing spondylitis (AS) is a common inflammatory arthritic condition. Overt inflammatory bowel disease (IBD) occurs in about 10% of AS patients, and in addition 70% of AS cases may have subclinical terminal ileitis. Spondyloarthritis is also common in IBD patients. We therefore tested Crohn's disease susceptibility genes for association with AS, aiming to identify pleiotropic genetic associations with both diseases. Genotyping was carried out using Sequenom and Applied Biosystems TaqMan and OpenArray technologies on 53 markers selected from 30 Crohn's disease associated genomic regions. We tested genotypes in a population of unrelated individual cases (n = 2,773) and controls (n = 2,215) of white European ancestry for association with AS. Statistical analysis was carried out using a Cochran-Armitage test for trend in PLINK. Strong association was detected at chr1q32 near KIF21B (rs11584383, P = 1.6 × 10(-10), odds ratio (OR) = 0.74, 95% CI:0.68-0.82). Association with disease was also detected for 2 variants within STAT3 (rs6503695, P = 4.6 × 10(-4). OR = 0.86 (95% CI:0.79-0.93); rs744166, P = 2.6 × 10(-5), OR = 0.84 (95% CI:0.77-0.91)). Association was confirmed for IL23R (rs11465804, P = 1.2 × 10(-5), OR = 0.65 (95% CI:0.54-0.79)), and further associations were detected for IL12B (rs10045431, P = 5.2 × 10(-5), OR = 0.83 (95% CI:0.76-0.91)), CDKAL1 (rs6908425, P = 1.1 × 10(-4), OR = 0.82 (95% CI:0.74-0.91)), LRRK2/MUC19 (rs11175593, P = 9.9 × 10(-5), OR = 1.92 (95% CI: 1.38-2.67)), and chr13q14 (rs3764147, P = 5.9 × 10(-4), OR = 1.19 (95% CI: 1.08-1.31)). Excluding cases with clinical IBD did not significantly affect these findings. This study identifies chr1q32 and STAT3 as ankylosing spondylitis susceptibility loci. It also further confirms association for IL23R and detects suggestive association with another 4 loci. STAT3 is a key signaling molecule within the Th17 lymphocyte differentiation pathway and further enhances the case for a major role of this T-lymphocyte subset in ankylosing spondylitis. Finally these findings suggest common aetiopathogenic pathways for AS and Crohn's disease and further highlight the involvement of common risk variants across multiple diseases.
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spelling doaj-art-e7e7a644f80b4c5cb5acdf7d3d2ee2a02025-08-20T03:07:21ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042010-12-01612e100119510.1371/journal.pgen.1001195Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.Patrick DanoyKarena PryceJohanna HadlerLinda A BradburyClaire FarrarJennifer PointonAustralo-Anglo-American Spondyloarthritis ConsortiumMichael WardMichael WeismanJohn D ReveilleB Paul WordsworthMillicent A StoneSpondyloarthritis Research Consortium of CanadaWalter P MaksymowychProton RahmanDafna GladmanRobert D InmanMatthew A BrownAnkylosing spondylitis (AS) is a common inflammatory arthritic condition. Overt inflammatory bowel disease (IBD) occurs in about 10% of AS patients, and in addition 70% of AS cases may have subclinical terminal ileitis. Spondyloarthritis is also common in IBD patients. We therefore tested Crohn's disease susceptibility genes for association with AS, aiming to identify pleiotropic genetic associations with both diseases. Genotyping was carried out using Sequenom and Applied Biosystems TaqMan and OpenArray technologies on 53 markers selected from 30 Crohn's disease associated genomic regions. We tested genotypes in a population of unrelated individual cases (n = 2,773) and controls (n = 2,215) of white European ancestry for association with AS. Statistical analysis was carried out using a Cochran-Armitage test for trend in PLINK. Strong association was detected at chr1q32 near KIF21B (rs11584383, P = 1.6 × 10(-10), odds ratio (OR) = 0.74, 95% CI:0.68-0.82). Association with disease was also detected for 2 variants within STAT3 (rs6503695, P = 4.6 × 10(-4). OR = 0.86 (95% CI:0.79-0.93); rs744166, P = 2.6 × 10(-5), OR = 0.84 (95% CI:0.77-0.91)). Association was confirmed for IL23R (rs11465804, P = 1.2 × 10(-5), OR = 0.65 (95% CI:0.54-0.79)), and further associations were detected for IL12B (rs10045431, P = 5.2 × 10(-5), OR = 0.83 (95% CI:0.76-0.91)), CDKAL1 (rs6908425, P = 1.1 × 10(-4), OR = 0.82 (95% CI:0.74-0.91)), LRRK2/MUC19 (rs11175593, P = 9.9 × 10(-5), OR = 1.92 (95% CI: 1.38-2.67)), and chr13q14 (rs3764147, P = 5.9 × 10(-4), OR = 1.19 (95% CI: 1.08-1.31)). Excluding cases with clinical IBD did not significantly affect these findings. This study identifies chr1q32 and STAT3 as ankylosing spondylitis susceptibility loci. It also further confirms association for IL23R and detects suggestive association with another 4 loci. STAT3 is a key signaling molecule within the Th17 lymphocyte differentiation pathway and further enhances the case for a major role of this T-lymphocyte subset in ankylosing spondylitis. Finally these findings suggest common aetiopathogenic pathways for AS and Crohn's disease and further highlight the involvement of common risk variants across multiple diseases.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1001195&type=printable
spellingShingle Patrick Danoy
Karena Pryce
Johanna Hadler
Linda A Bradbury
Claire Farrar
Jennifer Pointon
Australo-Anglo-American Spondyloarthritis Consortium
Michael Ward
Michael Weisman
John D Reveille
B Paul Wordsworth
Millicent A Stone
Spondyloarthritis Research Consortium of Canada
Walter P Maksymowych
Proton Rahman
Dafna Gladman
Robert D Inman
Matthew A Brown
Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
PLoS Genetics
title Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
title_full Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
title_fullStr Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
title_full_unstemmed Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
title_short Association of variants at 1q32 and STAT3 with ankylosing spondylitis suggests genetic overlap with Crohn's disease.
title_sort association of variants at 1q32 and stat3 with ankylosing spondylitis suggests genetic overlap with crohn s disease
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1001195&type=printable
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