Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation
Abstract Aberrant autophagy in vascular smooth muscle cells (VSMCs) is associated with the progression of vascular remodeling diseases caused by neointimal hyperplasia. Platelet-derived growth factor-BB (PDGF-BB)-induced vascular remodeling is accompanied by autophagy activation, however, the involv...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07580-4 |
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| author | Shi-Qing Mu Jia-Jie Lin Yu Wang Li-Yun Yang Sen Wang Zhao-Yi Wang An-Qi Zhao Wen-Jun Luo Zi-Qi Dong Yu-Guang Cao Ze-An Jiang Si-Fan Wang Shan-Hu Cao Li Meng Yang Li Shu-Yan Yang Shao-Guang Sun |
| author_facet | Shi-Qing Mu Jia-Jie Lin Yu Wang Li-Yun Yang Sen Wang Zhao-Yi Wang An-Qi Zhao Wen-Jun Luo Zi-Qi Dong Yu-Guang Cao Ze-An Jiang Si-Fan Wang Shan-Hu Cao Li Meng Yang Li Shu-Yan Yang Shao-Guang Sun |
| author_sort | Shi-Qing Mu |
| collection | DOAJ |
| description | Abstract Aberrant autophagy in vascular smooth muscle cells (VSMCs) is associated with the progression of vascular remodeling diseases caused by neointimal hyperplasia. Platelet-derived growth factor-BB (PDGF-BB)-induced vascular remodeling is accompanied by autophagy activation, however, the involvement of circular RNAs (circRNAs) remains unclear. Here, we show the role of PDGF-BB-regulated hsa_circ_0001304 (circ-1304) in neointimal hyperplasia and its potential involvement in VSMC autophagy, while also elucidating the potential mechanisms. Functionally, overexpression of circ-1304 promotes VSMC autophagy in vitro and exacerbates neointimal hyperplasia in vivo, and this exacerbation is accompanied by autophagy activation. Mechanistically, circ-1304 acts as a sponge for miR-636, resulting in increased protein levels of YTHDF2. Subsequently, the YTHDF2 protein promotes the degradation of mTOR mRNA by binding to the latter’s m6A modification sites. We demonstrate that PDGF-BB activates VSMC autophagy via circRNA regulation. Therefore, circ-1304 may serve as a potential therapeutic target for vascular remodeling diseases. |
| format | Article |
| id | doaj-art-e7d407e745fb44a38335fff68bc07db4 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-e7d407e745fb44a38335fff68bc07db42025-02-02T12:37:11ZengNature PortfolioCommunications Biology2399-36422025-01-018111110.1038/s42003-025-07580-4Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activationShi-Qing Mu0Jia-Jie Lin1Yu Wang2Li-Yun Yang3Sen Wang4Zhao-Yi Wang5An-Qi Zhao6Wen-Jun Luo7Zi-Qi Dong8Yu-Guang Cao9Ze-An Jiang10Si-Fan Wang11Shan-Hu Cao12Li Meng13Yang Li14Shu-Yan Yang15Shao-Guang Sun16Department of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityCore Facilities and Centers, Hebei Medical UniversityDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityBeijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of PediatricsDepartment of Biochemistry and Molecular Biology, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Key Laboratory of Forensic Medicine, Hebei Medical UniversityAbstract Aberrant autophagy in vascular smooth muscle cells (VSMCs) is associated with the progression of vascular remodeling diseases caused by neointimal hyperplasia. Platelet-derived growth factor-BB (PDGF-BB)-induced vascular remodeling is accompanied by autophagy activation, however, the involvement of circular RNAs (circRNAs) remains unclear. Here, we show the role of PDGF-BB-regulated hsa_circ_0001304 (circ-1304) in neointimal hyperplasia and its potential involvement in VSMC autophagy, while also elucidating the potential mechanisms. Functionally, overexpression of circ-1304 promotes VSMC autophagy in vitro and exacerbates neointimal hyperplasia in vivo, and this exacerbation is accompanied by autophagy activation. Mechanistically, circ-1304 acts as a sponge for miR-636, resulting in increased protein levels of YTHDF2. Subsequently, the YTHDF2 protein promotes the degradation of mTOR mRNA by binding to the latter’s m6A modification sites. We demonstrate that PDGF-BB activates VSMC autophagy via circRNA regulation. Therefore, circ-1304 may serve as a potential therapeutic target for vascular remodeling diseases.https://doi.org/10.1038/s42003-025-07580-4 |
| spellingShingle | Shi-Qing Mu Jia-Jie Lin Yu Wang Li-Yun Yang Sen Wang Zhao-Yi Wang An-Qi Zhao Wen-Jun Luo Zi-Qi Dong Yu-Guang Cao Ze-An Jiang Si-Fan Wang Shan-Hu Cao Li Meng Yang Li Shu-Yan Yang Shao-Guang Sun Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation Communications Biology |
| title | Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| title_full | Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| title_fullStr | Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| title_full_unstemmed | Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| title_short | Hsa_circ_0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| title_sort | hsa circ 0001304 promotes vascular neointimal hyperplasia accompanied by autophagy activation |
| url | https://doi.org/10.1038/s42003-025-07580-4 |
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