Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients
Measurements of antigen-specific T cell responses in chronic diseases are limited by low frequencies of antigen-specific cells in the peripheral blood. Therefore, attempts have been made to add costimulatory molecules such as anti-CD28 or IL-7/IL-15 to ELISPOT assays to increase sensitivity. While...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2008/590941 |
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| author | Stefanie Kuerten Tobias R. Schlingmann Tarvo Rajasalu Doychin N. Angelov Paul V. Lehmann Magdalena Tary-Lehmann |
| author_facet | Stefanie Kuerten Tobias R. Schlingmann Tarvo Rajasalu Doychin N. Angelov Paul V. Lehmann Magdalena Tary-Lehmann |
| author_sort | Stefanie Kuerten |
| collection | DOAJ |
| description | Measurements of antigen-specific T cell responses in chronic diseases are limited by low frequencies of antigen-specific cells in the peripheral blood. Therefore, attempts have been made to add costimulatory molecules such as anti-CD28 or IL-7/IL-15 to ELISPOT assays to increase sensitivity. While this approach has been successful under certain circumstances, results are often inconsistent. To date, there are no comprehensive studies directly comparing the in vitro effects of multiple costimulatory molecules in different disease settings. Therefore, in the present study we tested the effects of IL-7/IL-15, IFN-α, anti-ICOS, and anti-CD28 on antigen-specific T cell responses in patients infected with HCV or HIV versus healthy individuals. Our data show that none of the aforementioned molecules could significantly increase ELISPOT sensitivity, neither in HCV nor in HIV. Moreover, all of them caused false-positive responses to HCV and HIV antigens in healthy individuals. Our results question the broad use of in vitro costimulation. |
| format | Article |
| id | doaj-art-e7cb61565ba64c9b9bef16522915ae95 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-e7cb61565ba64c9b9bef16522915ae952025-02-03T01:09:45ZengWileyClinical and Developmental Immunology1740-25221740-25302008-01-01200810.1155/2008/590941590941Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected PatientsStefanie Kuerten0Tobias R. Schlingmann1Tarvo Rajasalu2Doychin N. Angelov3Paul V. Lehmann4Magdalena Tary-Lehmann5Institut für Anatomie I, Medizinische Fakultät der Universität zu Köln, Joseph-Stelzmann-Str. 9, 50931 Köln, GermanyDepartment of Pathology, Case Western Reserve University, Wolstein Building, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Pathology, Case Western Reserve University, Wolstein Building, 10900 Euclid Avenue, Cleveland, OH 44106, USAInstitut für Anatomie I, Medizinische Fakultät der Universität zu Köln, Joseph-Stelzmann-Str. 9, 50931 Köln, GermanyDepartment of Pathology, Case Western Reserve University, Wolstein Building, 10900 Euclid Avenue, Cleveland, OH 44106, USADepartment of Pathology, Case Western Reserve University, Wolstein Building, 10900 Euclid Avenue, Cleveland, OH 44106, USAMeasurements of antigen-specific T cell responses in chronic diseases are limited by low frequencies of antigen-specific cells in the peripheral blood. Therefore, attempts have been made to add costimulatory molecules such as anti-CD28 or IL-7/IL-15 to ELISPOT assays to increase sensitivity. While this approach has been successful under certain circumstances, results are often inconsistent. To date, there are no comprehensive studies directly comparing the in vitro effects of multiple costimulatory molecules in different disease settings. Therefore, in the present study we tested the effects of IL-7/IL-15, IFN-α, anti-ICOS, and anti-CD28 on antigen-specific T cell responses in patients infected with HCV or HIV versus healthy individuals. Our data show that none of the aforementioned molecules could significantly increase ELISPOT sensitivity, neither in HCV nor in HIV. Moreover, all of them caused false-positive responses to HCV and HIV antigens in healthy individuals. Our results question the broad use of in vitro costimulation.http://dx.doi.org/10.1155/2008/590941 |
| spellingShingle | Stefanie Kuerten Tobias R. Schlingmann Tarvo Rajasalu Doychin N. Angelov Paul V. Lehmann Magdalena Tary-Lehmann Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients Clinical and Developmental Immunology |
| title | Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients |
| title_full | Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients |
| title_fullStr | Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients |
| title_full_unstemmed | Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients |
| title_short | Lack of Disease Specificity Limits the Usefulness of In Vitro Costimulation in HIV- and HCV-Infected Patients |
| title_sort | lack of disease specificity limits the usefulness of in vitro costimulation in hiv and hcv infected patients |
| url | http://dx.doi.org/10.1155/2008/590941 |
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