Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels
Possible links have been investigated between activation of protein kinase C (PKC) and endothelin (ET) production by small blood vessels. Perfusion pressures were recorded from rat isolated mesenteric artery, with or without the small intestine attached, before and after addition to the perfusate of...
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S096293519500010X |
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| _version_ | 1832555819143528448 |
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| author | A. M. Northover B. J. Northover |
| author_facet | A. M. Northover B. J. Northover |
| author_sort | A. M. Northover |
| collection | DOAJ |
| description | Possible links have been investigated between activation of protein kinase C (PKC) and endothelin (ET) production by small blood vessels. Perfusion pressures were recorded from rat isolated mesenteric artery, with or without the small intestine attached, before and after addition to the perfusate of either ET-1, ET-3 or the PKC activator 12-deoxyphorbol 13-phenylacetate (DOPPA). Rises in perfusion pressure in response to ET-1 (10−8 M)or DOPPA (10−6 M) were reduced significantly by pre-treatment with either the ETA receptor antagonist PD151242 (10−6 M) or the PKC inhibitor Ro 31-8220 (10−6 M). ET-3 (10−8 M) had a significant, albeit small, effect only when the gut was still attached to the mesentery. Inthis latter preparation ET-1 and DOPPA increased the permeability of villi microvessels to colloidal carbon in the perfusate. This effect of DOPPA was reduced by pre-treatment with either PD151242 or Ro 31-8220, but the effects of ET-1 were reduced significantly only by Ro 31-8220. ET-3 (10−8 M) was without effect. The results suggest a possible bi-directional link between ETA receptors and PKC in the intestinal vasculature. |
| format | Article |
| id | doaj-art-e7c8161bef1e4ddf9dd57224b3076247 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e7c8161bef1e4ddf9dd57224b30762472025-02-03T05:47:13ZengWileyMediators of Inflammation0962-93511466-18611995-01-0141555910.1155/S096293519500010XPossible bi-directional link between ETA receptors and protein kinase C in rat blood vesselsA. M. Northover0B. J. Northover1Department of Pharmaceutical Sciences, School of Applied Sciences, De Montfort University, Leicester LE1 9BH, UKDepartment of Pharmaceutical Sciences, School of Applied Sciences, De Montfort University, Leicester LE1 9BH, UKPossible links have been investigated between activation of protein kinase C (PKC) and endothelin (ET) production by small blood vessels. Perfusion pressures were recorded from rat isolated mesenteric artery, with or without the small intestine attached, before and after addition to the perfusate of either ET-1, ET-3 or the PKC activator 12-deoxyphorbol 13-phenylacetate (DOPPA). Rises in perfusion pressure in response to ET-1 (10−8 M)or DOPPA (10−6 M) were reduced significantly by pre-treatment with either the ETA receptor antagonist PD151242 (10−6 M) or the PKC inhibitor Ro 31-8220 (10−6 M). ET-3 (10−8 M) had a significant, albeit small, effect only when the gut was still attached to the mesentery. Inthis latter preparation ET-1 and DOPPA increased the permeability of villi microvessels to colloidal carbon in the perfusate. This effect of DOPPA was reduced by pre-treatment with either PD151242 or Ro 31-8220, but the effects of ET-1 were reduced significantly only by Ro 31-8220. ET-3 (10−8 M) was without effect. The results suggest a possible bi-directional link between ETA receptors and PKC in the intestinal vasculature.http://dx.doi.org/10.1155/S096293519500010X |
| spellingShingle | A. M. Northover B. J. Northover Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels Mediators of Inflammation |
| title | Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels |
| title_full | Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels |
| title_fullStr | Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels |
| title_full_unstemmed | Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels |
| title_short | Possible bi-directional link between ETA receptors and protein kinase C in rat blood vessels |
| title_sort | possible bi directional link between eta receptors and protein kinase c in rat blood vessels |
| url | http://dx.doi.org/10.1155/S096293519500010X |
| work_keys_str_mv | AT amnorthover possiblebidirectionallinkbetweenetareceptorsandproteinkinasecinratbloodvessels AT bjnorthover possiblebidirectionallinkbetweenetareceptorsandproteinkinasecinratbloodvessels |