A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-05-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2019-0172 |
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| _version_ | 1850151662137638912 |
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| author | Shan Wei Brynn Levy Nataly Hoffman Claudia Cujar Reunet Rodney-Sandy Ronald Wapner Mary D'Alton Zev Williams |
| author_facet | Shan Wei Brynn Levy Nataly Hoffman Claudia Cujar Reunet Rodney-Sandy Ronald Wapner Mary D'Alton Zev Williams |
| author_sort | Shan Wei |
| collection | DOAJ |
| description | Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications. |
| format | Article |
| id | doaj-art-e7c45da6d14e4935b68c8df196a57cdc |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-e7c45da6d14e4935b68c8df196a57cdc2025-08-20T02:26:09ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-05-0168524024410.2144/btn-2019-0172A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissueShan Wei0Brynn Levy1Nataly Hoffman2Claudia Cujar3Reunet Rodney-Sandy4Ronald Wapner5Mary D'Alton6Zev Williams71Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA2Department of Pathology & Cell Biology, Columbia University Medical Center, New York, NY 10032, USA3Clinical Cytogenetics Laboratory, Columbia University Medical Center & the New York Presbyterian Hospital, New York, NY 10032, USA2Department of Pathology & Cell Biology, Columbia University Medical Center, New York, NY 10032, USA1Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA1Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA1Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USA1Department of Obstetrics & Gynecology, Columbia University Irving Medical Center, New York, NY 10032, USAConventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.https://www.future-science.com/doi/10.2144/btn-2019-0172genetic testingrapid clinical diagnosticsrapid tissue gDNA extractiontissue processing |
| spellingShingle | Shan Wei Brynn Levy Nataly Hoffman Claudia Cujar Reunet Rodney-Sandy Ronald Wapner Mary D'Alton Zev Williams A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue BioTechniques genetic testing rapid clinical diagnostics rapid tissue gDNA extraction tissue processing |
| title | A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue |
| title_full | A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue |
| title_fullStr | A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue |
| title_full_unstemmed | A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue |
| title_short | A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue |
| title_sort | rapid and simple bead bashing based method for genomic dna extraction from mammalian tissue |
| topic | genetic testing rapid clinical diagnostics rapid tissue gDNA extraction tissue processing |
| url | https://www.future-science.com/doi/10.2144/btn-2019-0172 |
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