A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue

A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume...

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Bibliographic Details
Main Authors: Shan Wei, Brynn Levy, Nataly Hoffman, Claudia Cujar, Reunet Rodney-Sandy, Ronald Wapner, Mary D'Alton, Zev Williams
Format: Article
Language:English
Published: Taylor & Francis Group 2020-05-01
Series:BioTechniques
Subjects:
genetic testing
rapid clinical diagnostics
rapid tissue gDNA extraction
tissue processing
Online Access:https://www.future-science.com/doi/10.2144/btn-2019-0172
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Summary:Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.
ISSN:0736-6205
1940-9818

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