Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone
This study was aimed to determine the immersion frequency of recombinant growth hormone (rGH) in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, was immersed in 30 ppt salt water for 2 minutes, and then transferred into 200 mL of 9 ppt salt...
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Asosiasi Sains Akuakultur Indonesia
2013-11-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/7194 |
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| author | Aras Syazili . Irmawati . Alimuddin Komar Sumantadinata |
| author_facet | Aras Syazili . Irmawati . Alimuddin Komar Sumantadinata |
| author_sort | Aras Syazili |
| collection | DOAJ |
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This study was aimed to determine the immersion frequency of recombinant growth hormone (rGH) in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, was immersed in 30 ppt salt water for 2 minutes, and then transferred into 200 mL of 9 ppt salt water containing 30 mg/L rGH in inclusion bodies form. Immersion was performed for 60 minutes and repeated once a week for two, three, four, and five weeks. As the control, fish was immersed with inclusion bodies without rGH (control-1), bovine serum albumin (control-2), 9ppt saline water without rGH (control-3) and in freshwater (control-4). Fish were kept in 7L aquaria during the first four weeks, transferred into 50L aquaria for two weeks, and then in hapa (2×1×1 m3 in size) for two weeks. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.23±0.19 g) was obtained at four times (four weeks) immersion treatment. Similar body weight of fish in two times (1.72±0.29 g), three times (1.68±0.34 g), and five times (1.81±0.17 g) immersion was obtained. Fish body weight of all controls was lower than the rGH-immersed fish. Average body weight of four time rGH-immersed fish (4.01 g) remained higher than other treatment and control (1.96 to 2.36 g) on 8th week of fish rearing. Survival of four times (96.67±1.15%), and five times rGH-immersion fish (95.33±4.16%) was similar, and higher than other treatments and controls (77.33 to 89.33%). Thus, higher growth rate and survival of giant gourami juvenile could be achieved by four times rGH immersion.
Keywords: recombinant protein, growth hormone, immersion frequency, giant gourami |
| format | Article |
| id | doaj-art-e7b5cb15c77b4bdc88369afc9c2f52e0 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2013-11-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-e7b5cb15c77b4bdc88369afc9c2f52e02025-01-24T18:00:44ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002013-11-0111110.19027/jai.11.23-276262Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormoneAras Syazili0. Irmawati1. Alimuddin2Komar Sumantadinata3Department of AquacultureFPIK, Universitas Hasanuddin, Makassar. Jl. Perintis Kemerdekaan Km.10 Makassar, 90245, Sulawesi Selatan, IndonesiaDepartemen Budidaya Perairan, FPIK, IPB. Kampus IPB Darmaga Bogor 16680Departemen Budidaya Perairan, FPIK, IPB. Kampus IPB Darmaga Bogor 16680 This study was aimed to determine the immersion frequency of recombinant growth hormone (rGH) in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, was immersed in 30 ppt salt water for 2 minutes, and then transferred into 200 mL of 9 ppt salt water containing 30 mg/L rGH in inclusion bodies form. Immersion was performed for 60 minutes and repeated once a week for two, three, four, and five weeks. As the control, fish was immersed with inclusion bodies without rGH (control-1), bovine serum albumin (control-2), 9ppt saline water without rGH (control-3) and in freshwater (control-4). Fish were kept in 7L aquaria during the first four weeks, transferred into 50L aquaria for two weeks, and then in hapa (2×1×1 m3 in size) for two weeks. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.23±0.19 g) was obtained at four times (four weeks) immersion treatment. Similar body weight of fish in two times (1.72±0.29 g), three times (1.68±0.34 g), and five times (1.81±0.17 g) immersion was obtained. Fish body weight of all controls was lower than the rGH-immersed fish. Average body weight of four time rGH-immersed fish (4.01 g) remained higher than other treatment and control (1.96 to 2.36 g) on 8th week of fish rearing. Survival of four times (96.67±1.15%), and five times rGH-immersion fish (95.33±4.16%) was similar, and higher than other treatments and controls (77.33 to 89.33%). Thus, higher growth rate and survival of giant gourami juvenile could be achieved by four times rGH immersion. Keywords: recombinant protein, growth hormone, immersion frequency, giant gouramihttps://journal.ipb.ac.id/index.php/jai/article/view/7194 |
| spellingShingle | Aras Syazili . Irmawati . Alimuddin Komar Sumantadinata Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone Jurnal Akuakultur Indonesia |
| title | Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| title_full | Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| title_fullStr | Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| title_full_unstemmed | Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| title_short | Growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| title_sort | growth and survival of giant gourami juvenile immerse indifferent frequencies using recombinant growth hormone |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/7194 |
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