Hybrid layer-by-layer assembly of AuNPs/NSF composite for electrochemical detection of miRNA-196a
Abstract Detection of microRNA-196a (miRNA-196a) is crucial in cancer research, enabling early diagnosis and providing guidance for individualized treatment. In this work, we employed a naturally occurring negatively charged nano silk fibroin (NSF) with high mechanical properties, biocompatibility,...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-11-01
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| Series: | Discover Nano |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s11671-024-04127-0 |
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| Summary: | Abstract Detection of microRNA-196a (miRNA-196a) is crucial in cancer research, enabling early diagnosis and providing guidance for individualized treatment. In this work, we employed a naturally occurring negatively charged nano silk fibroin (NSF) with high mechanical properties, biocompatibility, and conductivity to be encapsulated with a positively charged gold nanoparticles (AuNPs) were used as film-forming materials for electrostatic layer-by-layer self-assembly to modify the working electrode of the screen-printed carbon electrode (SPCE). Under the optimized experimental conditions, the uniformly distributed AuNPs on the surface of the multilayer film modified SPCE (AuNPs/NSF)5.5/SPCE combined with the sulfhydryl-modified capture probe cp-DNA through gold-sulfur bonds. Furthermore, miRNA-196a is specifically captured through complementary base pairing to achieve highly sensitive and specific detection. (AuNPs/NSF)5.5/SPCE electrode can detect miRNA-196a in a concentration range of 1.0 × 10−13 to 1.0 × 10−6 M, and the calculated detection limit is 4.63 × 10−14 M when the signal-to-noise ratio is 3. The obtained results showed that the (AuNPs/NSF)5.5/SPCE has excellent selectivity and good stability over time. |
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| ISSN: | 2731-9229 |