3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis
Fabricating bone tissue engineering substitutes with functional activity remains a challenge for bone defect repair requiring coordinated coupling between osteogenesis and angiogenesis. In this research, we evaluated and analyzed magnesium silicate/β-Tricalcium phosphate (MS/β-TCP) scaffold on angio...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1518145/full |
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| author | Lulu Wang Mingkui Shen Zhongxin Tang Jun Tan Kuankuan Li Haijun Ma |
| author_facet | Lulu Wang Mingkui Shen Zhongxin Tang Jun Tan Kuankuan Li Haijun Ma |
| author_sort | Lulu Wang |
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| description | Fabricating bone tissue engineering substitutes with functional activity remains a challenge for bone defect repair requiring coordinated coupling between osteogenesis and angiogenesis. In this research, we evaluated and analyzed magnesium silicate/β-Tricalcium phosphate (MS/β-TCP) scaffold on angiogenesis and bone regeneration in vitro and in vivo, and the mechanism of its action were described. Achieving magnesium and silicon ions sustained release, 3D printed MS/β-TCP scaffolds possessed appropriate mechanical properties and had excellent biocompatibility that was suitable for osteoblastic MC3T3-E1 cells and human umbilical vein endothelial cells (HUVECs) with proliferation, adhesion, and migration. Combined techniques of Transwell co-culture, we studied the effect of MS/β-TCP scaffold activated cell-level specific regulatory network, which promotes the osteogenic differentiation of MC3T3-E1 and the endothelial formation of HUVEC by significantly up-regulating the expression of related genes and proteins. In addition, RNA sequencing (RNA-seq) revealed MS/β-TCP scaffold plays a dual role in osteogenesis and angiogenesis by activating PI3K/Akt signal pathway, whereas the expression of genes and proteins associated with osteogenesis and angiogenesis was significantly downregulated the PI3K/Akt signaling pathway was inhibited. Additionally, in vivo studies showed that MS/β-TCP scaffolds increased the growth of vascular and promoted the bone regeneration at the bone defect sites in rats. In summary, 3D printed MS/β-TCP scaffolds with effectively osteogenic and angiogenic induction will be an ideal bone substitute applied in bone defect repair for clinical application in the future. |
| format | Article |
| id | doaj-art-e7a9edbb2cd842f0a58b6c2e1147e2ae |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-e7a9edbb2cd842f0a58b6c2e1147e2ae2025-01-31T06:39:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.151814515181453D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesisLulu Wang0Mingkui Shen1Zhongxin Tang2Jun Tan3Kuankuan Li4Haijun Ma5Department of Plastic Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan Province, Zhengzhou, ChinaFabricating bone tissue engineering substitutes with functional activity remains a challenge for bone defect repair requiring coordinated coupling between osteogenesis and angiogenesis. In this research, we evaluated and analyzed magnesium silicate/β-Tricalcium phosphate (MS/β-TCP) scaffold on angiogenesis and bone regeneration in vitro and in vivo, and the mechanism of its action were described. Achieving magnesium and silicon ions sustained release, 3D printed MS/β-TCP scaffolds possessed appropriate mechanical properties and had excellent biocompatibility that was suitable for osteoblastic MC3T3-E1 cells and human umbilical vein endothelial cells (HUVECs) with proliferation, adhesion, and migration. Combined techniques of Transwell co-culture, we studied the effect of MS/β-TCP scaffold activated cell-level specific regulatory network, which promotes the osteogenic differentiation of MC3T3-E1 and the endothelial formation of HUVEC by significantly up-regulating the expression of related genes and proteins. In addition, RNA sequencing (RNA-seq) revealed MS/β-TCP scaffold plays a dual role in osteogenesis and angiogenesis by activating PI3K/Akt signal pathway, whereas the expression of genes and proteins associated with osteogenesis and angiogenesis was significantly downregulated the PI3K/Akt signaling pathway was inhibited. Additionally, in vivo studies showed that MS/β-TCP scaffolds increased the growth of vascular and promoted the bone regeneration at the bone defect sites in rats. In summary, 3D printed MS/β-TCP scaffolds with effectively osteogenic and angiogenic induction will be an ideal bone substitute applied in bone defect repair for clinical application in the future.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1518145/fullmagnesium silicateosteogenesis and angiogenesisbone tissue engineeringRNA sequencingbone regeneration |
| spellingShingle | Lulu Wang Mingkui Shen Zhongxin Tang Jun Tan Kuankuan Li Haijun Ma 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis Frontiers in Bioengineering and Biotechnology magnesium silicate osteogenesis and angiogenesis bone tissue engineering RNA sequencing bone regeneration |
| title | 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| title_full | 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| title_fullStr | 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| title_full_unstemmed | 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| title_short | 3D printed magnesium silicate/β-tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| title_sort | 3d printed magnesium silicate β tricalcium phosphate scaffolds promote coupled osteogenesis and angiogenesis |
| topic | magnesium silicate osteogenesis and angiogenesis bone tissue engineering RNA sequencing bone regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1518145/full |
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