The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice
The retinal degeneration 11 (rd11) mouse is a new animal model with rapid photoreceptor degeneration. The long-term efficacy of gene therapy has a direct relationship with the onset of photoreceptor degeneration or apoptosis, whereas the degeneration or apoptosis patterns of photoreceptors are still...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/9721362 |
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| author | Hua Zhang Xia Li Xufeng Dai Juanjuan Han Yangyang Zhang Yan Qi Ying He Yan Liu Bo Chang Ji-jing Pang |
| author_facet | Hua Zhang Xia Li Xufeng Dai Juanjuan Han Yangyang Zhang Yan Qi Ying He Yan Liu Bo Chang Ji-jing Pang |
| author_sort | Hua Zhang |
| collection | DOAJ |
| description | The retinal degeneration 11 (rd11) mouse is a new animal model with rapid photoreceptor degeneration. The long-term efficacy of gene therapy has a direct relationship with the onset of photoreceptor degeneration or apoptosis, whereas the degeneration or apoptosis patterns of photoreceptors are still unclear in rd11 mice. The distribution patterns of cone function-related L- and S-opsin were examined by immunofluorescence staining, and the apoptosis was performed by TUNEL assay in rd11 mice. The expression pattern of L-opsin or S-opsin in rd11 retina at postnatal day (P) 14 was similar to the pattern observed in wildtype retina. With increasing age, the expression of L-opsin and S-opsin, especially S-opsin, decreased significantly in rd11 mice. The degeneration of L-opsin began around the optic nerve and expanded to the periphery of the retina, from the ventral/nasal to dorsal/temporal retina, whereas the expression of S-opsin gradually decreased from the dorsal/temporal to ventral/nasal retina. Apoptotic signal appeared at P14 and was strongest at P28 of rd11 mice. The key genes associated with apoptosis confirmed those changes. These indicated that the degeneration and apoptosis of cone photoreceptors began at P14 of rd11 mice, which was a key point for gene therapy. |
| format | Article |
| id | doaj-art-e764f107064d4de7b49277b67109b46b |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-e764f107064d4de7b49277b67109b46b2025-02-03T01:24:20ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/97213629721362The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 MiceHua Zhang0Xia Li1Xufeng Dai2Juanjuan Han3Yangyang Zhang4Yan Qi5Ying He6Yan Liu7Bo Chang8Ji-jing Pang9School of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, ChinaSchool of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaFenyang College, Shanxi Medical University, Fenyang, Shanxi 032200, ChinaSchool of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaQingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Science, Qingdao 266000, ChinaSchool of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaSchool of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaThe Jackson Laboratory, Bar Harbor, ME, USASchool of Ophthalmology & Optometry, The Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaThe retinal degeneration 11 (rd11) mouse is a new animal model with rapid photoreceptor degeneration. The long-term efficacy of gene therapy has a direct relationship with the onset of photoreceptor degeneration or apoptosis, whereas the degeneration or apoptosis patterns of photoreceptors are still unclear in rd11 mice. The distribution patterns of cone function-related L- and S-opsin were examined by immunofluorescence staining, and the apoptosis was performed by TUNEL assay in rd11 mice. The expression pattern of L-opsin or S-opsin in rd11 retina at postnatal day (P) 14 was similar to the pattern observed in wildtype retina. With increasing age, the expression of L-opsin and S-opsin, especially S-opsin, decreased significantly in rd11 mice. The degeneration of L-opsin began around the optic nerve and expanded to the periphery of the retina, from the ventral/nasal to dorsal/temporal retina, whereas the expression of S-opsin gradually decreased from the dorsal/temporal to ventral/nasal retina. Apoptotic signal appeared at P14 and was strongest at P28 of rd11 mice. The key genes associated with apoptosis confirmed those changes. These indicated that the degeneration and apoptosis of cone photoreceptors began at P14 of rd11 mice, which was a key point for gene therapy.http://dx.doi.org/10.1155/2017/9721362 |
| spellingShingle | Hua Zhang Xia Li Xufeng Dai Juanjuan Han Yangyang Zhang Yan Qi Ying He Yan Liu Bo Chang Ji-jing Pang The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice Journal of Ophthalmology |
| title | The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice |
| title_full | The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice |
| title_fullStr | The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice |
| title_full_unstemmed | The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice |
| title_short | The Degeneration and Apoptosis Patterns of Cone Photoreceptors in rd11 Mice |
| title_sort | degeneration and apoptosis patterns of cone photoreceptors in rd11 mice |
| url | http://dx.doi.org/10.1155/2017/9721362 |
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