A Spectroscopic Methodology to Early Detection of Urinary Tract Infections
Healthcare-associated infections (HAI) are a critical public health problem, with 30 to 40% of infections related to the urinary tract system. These urinary tract infections (UTIs) are considered one of the most common microbial infections in hospital settings and everyday community contexts, where...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/1424-8220/25/2/400 |
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| author | Ana F. N. S. Mendes Nuno Matela João M. P. Coelho Joaquim T. Marquês |
| author_facet | Ana F. N. S. Mendes Nuno Matela João M. P. Coelho Joaquim T. Marquês |
| author_sort | Ana F. N. S. Mendes |
| collection | DOAJ |
| description | Healthcare-associated infections (HAI) are a critical public health problem, with 30 to 40% of infections related to the urinary tract system. These urinary tract infections (UTIs) are considered one of the most common microbial infections in hospital settings and everyday community contexts, where approximately 80% are highly correlated with urinary catheter insertion, i.e., catheter-associated urinary tract infections (CAUTIs). Considering that 15 to 25% of hospitalised patients need to be catheterised during their treatments and most CAUTIs are asymptomatic, it results in a tremendous challenge to provide an early diagnosis of CAUTI and therefore initiate its treatment. The lack of standardised methods as a first step for urine monitoring and early detection of UTIs is the driving force of this work, which aims to explore the potential of absorption and fluorescence spectroscopic methodologies to detect UTIs. Urine samples were used without any previous treatment to target the most straightforward testing protocol possible. In this work, we successfully developed a powerful methodology that combines ratiometric fluorescence spectroscopy measurements and transmittance at 600 nm to distinguish healthy urine from infected urine. The complementary use of fluorescence spectroscopy and transmittance is what makes the new methodology we propose such a powerful approach to monitor urine samples and provide early detection of UTIs since it provides a quantitative analysis of both healthy and infected urine. |
| format | Article |
| id | doaj-art-e754b346bff8467e975e65f9fcd4f76c |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-e754b346bff8467e975e65f9fcd4f76c2025-01-24T13:48:47ZengMDPI AGSensors1424-82202025-01-0125240010.3390/s25020400A Spectroscopic Methodology to Early Detection of Urinary Tract InfectionsAna F. N. S. Mendes0Nuno Matela1João M. P. Coelho2Joaquim T. Marquês3Instituto de Biofísica e Engenharia Biomédica (IBEB), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalInstituto de Biofísica e Engenharia Biomédica (IBEB), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalInstituto de Biofísica e Engenharia Biomédica (IBEB), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalCentro de Química Estrutural, Institute of Molecular Sciences, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalHealthcare-associated infections (HAI) are a critical public health problem, with 30 to 40% of infections related to the urinary tract system. These urinary tract infections (UTIs) are considered one of the most common microbial infections in hospital settings and everyday community contexts, where approximately 80% are highly correlated with urinary catheter insertion, i.e., catheter-associated urinary tract infections (CAUTIs). Considering that 15 to 25% of hospitalised patients need to be catheterised during their treatments and most CAUTIs are asymptomatic, it results in a tremendous challenge to provide an early diagnosis of CAUTI and therefore initiate its treatment. The lack of standardised methods as a first step for urine monitoring and early detection of UTIs is the driving force of this work, which aims to explore the potential of absorption and fluorescence spectroscopic methodologies to detect UTIs. Urine samples were used without any previous treatment to target the most straightforward testing protocol possible. In this work, we successfully developed a powerful methodology that combines ratiometric fluorescence spectroscopy measurements and transmittance at 600 nm to distinguish healthy urine from infected urine. The complementary use of fluorescence spectroscopy and transmittance is what makes the new methodology we propose such a powerful approach to monitor urine samples and provide early detection of UTIs since it provides a quantitative analysis of both healthy and infected urine.https://www.mdpi.com/1424-8220/25/2/400urinary tract infectionsurine monitoringfluorescence spectroscopyratiometric measurementstransmittance |
| spellingShingle | Ana F. N. S. Mendes Nuno Matela João M. P. Coelho Joaquim T. Marquês A Spectroscopic Methodology to Early Detection of Urinary Tract Infections Sensors urinary tract infections urine monitoring fluorescence spectroscopy ratiometric measurements transmittance |
| title | A Spectroscopic Methodology to Early Detection of Urinary Tract Infections |
| title_full | A Spectroscopic Methodology to Early Detection of Urinary Tract Infections |
| title_fullStr | A Spectroscopic Methodology to Early Detection of Urinary Tract Infections |
| title_full_unstemmed | A Spectroscopic Methodology to Early Detection of Urinary Tract Infections |
| title_short | A Spectroscopic Methodology to Early Detection of Urinary Tract Infections |
| title_sort | spectroscopic methodology to early detection of urinary tract infections |
| topic | urinary tract infections urine monitoring fluorescence spectroscopy ratiometric measurements transmittance |
| url | https://www.mdpi.com/1424-8220/25/2/400 |
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